{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,27]],"date-time":"2025-09-27T21:51:51Z","timestamp":1759009911871},"reference-count":40,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2014,4,1]],"date-time":"2014-04-01T00:00:00Z","timestamp":1396310400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2014,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Vibrio cholerae<\/jats:named-content>\n            serogroup O139 was first identified in 1992 in India and Bangladesh, in association with major epidemics of cholera in both countries; cases were noted shortly thereafter in China. We characterized 211\n            <jats:named-content content-type=\"genus-species\">V. cholerae<\/jats:named-content>\n            O139 isolates that were isolated at multiple sites in China between 1993 and 2012 from patients (\n            <jats:italic>n<\/jats:italic>\n            = 92) and the environment (\n            <jats:italic>n<\/jats:italic>\n            = 119). Among clinical isolates, 88 (95.7%) of 92 were toxigenic, compared with 47 (39.5%) of 119 environmental isolates. Toxigenic isolates carried the El Tor CTX prophage and toxin-coregulated pilus A gene (\n            <jats:italic>tcpA<\/jats:italic>\n            ), as well as the\n            <jats:named-content content-type=\"genus-species\">Vibrio<\/jats:named-content>\n            seventh pandemic island I (VSP-I) and VSP-II. Among a subset of 42 toxigenic isolates screened by multilocus sequence typing (MLST), all were in the same sequence type as a clinical isolate (MO45) from the original Indian outbreak. Nontoxigenic isolates, in contrast, generally lacked VSP-I and -II, and fell within13 additional sequence types in two clonal complexes distinct from the toxigenic isolates. In further pulsed-field gel electrophoresis (PFGE) (with NotI digestion) studies, toxigenic isolates formed 60 pulsotypes clustered in one group, while the nontoxigenic isolates formed 43 pulsotypes which clustered into 3 different groups. Our data suggest that toxigenic O139 isolates from widely divergent geographic locations, while showing some diversity, have maintained a relatively tight clonal structure across a 20-year time span. Nontoxigenic isolates, in contrast, exhibited greater diversity, with multiple clonal lineages, than did their toxigenic counterparts.\n          <\/jats:p>","DOI":"10.1128\/jcm.03354-13","type":"journal-article","created":{"date-parts":[[2014,1,23]],"date-time":"2014-01-23T04:38:01Z","timestamp":1390451881000},"page":"1146-1152","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":20,"title":["A Molecular Surveillance Reveals the Prevalence of Vibrio cholerae O139 Isolates in China from 1993 to 2012"],"prefix":"10.1128","volume":"52","author":[{"given":"Ping","family":"Zhang","sequence":"first","affiliation":[{"name":"National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention\/State Key Laboratory for Infectious Disease Prevention and Control, Beijing, China, and Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haijian","family":"Zhou","sequence":"additional","affiliation":[{"name":"National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention\/State Key Laboratory for Infectious Disease Prevention and Control, Beijing, China, and Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Baowei","family":"Diao","sequence":"additional","affiliation":[{"name":"National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention\/State Key Laboratory for Infectious Disease Prevention and Control, Beijing, China, and Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fengjuan","family":"Li","sequence":"additional","affiliation":[{"name":"National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention\/State Key Laboratory for Infectious Disease Prevention and Control, Beijing, China, and Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pengcheng","family":"Du","sequence":"additional","affiliation":[{"name":"National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention\/State Key Laboratory for Infectious Disease Prevention and Control, Beijing, China, and Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Li","sequence":"additional","affiliation":[{"name":"National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention\/State Key Laboratory for Infectious Disease Prevention and Control, Beijing, China, and Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Biao","family":"Kan","sequence":"additional","affiliation":[{"name":"National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention\/State Key Laboratory for Infectious Disease Prevention and Control, Beijing, China, and Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr.","given":"J. 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