{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T10:48:54Z","timestamp":1789555734611,"version":"build-2803163510"},"reference-count":35,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2016,7,1]],"date-time":"2016-07-01T00:00:00Z","timestamp":1467331200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Stanford ITI Seed Grant"},{"name":"Beijing Top Time Science"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2016,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Toxoplasmosis is an infection caused by the protozoan parasite\n            <jats:named-content content-type=\"genus-species\">Toxoplasma gondii<\/jats:named-content>\n            that can lead to severe sequelae in the fetus during pregnancy. Definitive serologic diagnosis of the infection during gestation is made mostly by detecting\n            <jats:named-content content-type=\"genus-species\">T. gondii<\/jats:named-content>\n            -specific antibodies, including IgG and IgM, individually in a single serum sample by using commercially available kits. The IgA test is used by some laboratories as an additional marker of acute infection. Most of the commercial tests have failed to reach 100% correlation with the reference method, the Sabin-Feldman dye test for the detection of\n            <jats:named-content content-type=\"genus-species\">Toxoplasma<\/jats:named-content>\n            IgG antibodies. For\n            <jats:named-content content-type=\"genus-species\">Toxoplasma<\/jats:named-content>\n            IgM and IgA antibodies, there is no reference method and their evaluation is done by comparing the results of one assay to those of another. There is a need for multiplexed assay platforms, as the serological diagnosis of\n            <jats:named-content content-type=\"genus-species\">T. gondii<\/jats:named-content>\n            infection does not rely on the detection of a single Ig subtype. Here we describe the development of a plasmonic gold chip with vast fluorescence enhancement in the near-infrared region for simultaneous detection of IgG, IgM, and IgA antibodies against\n            <jats:named-content content-type=\"genus-species\">T. gondii<\/jats:named-content>\n            in an \u223c1-\u03bcl serum or whole-blood sample. When 168 samples were tested on this platform, IgG antibody detection sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) were all 100%. IgM antibody detection achieved 97.6% sensitivity and 96.9% specificity with a 90.9% PPV and a 99.2% NPV. Thus, the nanoscience-based plasmonic gold platform enables a high-performance, low-cost, multiplexed assay requiring ultrasmall blood volumes, paving the way for the implementation of universal screening for toxoplasmosis infection during gestation.\n          <\/jats:p>","DOI":"10.1128\/jcm.03371-15","type":"journal-article","created":{"date-parts":[[2016,3,24]],"date-time":"2016-03-24T04:26:28Z","timestamp":1458793588000},"page":"1726-1733","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":36,"title":["Multiplexed Anti-Toxoplasma IgG, IgM, and IgA Assay on Plasmonic Gold Chips: towards Making Mass Screening Possible with Dye Test Precision"],"prefix":"10.1128","volume":"54","author":[{"given":"Xiaoyang","family":"Li","sequence":"first","affiliation":[{"name":"Department of Chemistry, Stanford University, Stanford, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christelle","family":"Pomares","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Stanford University, Stanford, California, USA"},{"name":"Palo Alto Medical Foundation Toxoplasma Serology Laboratory, Palo Alto, California, USA"},{"name":"INSERM, U1065, Centre M\u00e9diterran\u00e9en de M\u00e9decine Mol\u00e9culaire, C3M, Toxines Microbiennes dans la Relation H\u00f4te Pathog\u00e8nes\u2014Universit\u00e9 de Nice Sophia Antipolis, Facult\u00e9 de M\u00e9decine, Nice, France"},{"name":"Parasitologie-Mycologie, Centre Hospitalier Universitaire l'Archet, Nice, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G\u00e9raldine","family":"Gonfrier","sequence":"additional","affiliation":[{"name":"INSERM, U1065, Centre M\u00e9diterran\u00e9en de M\u00e9decine Mol\u00e9culaire, C3M, Toxines Microbiennes dans la Relation H\u00f4te Pathog\u00e8nes\u2014Universit\u00e9 de Nice Sophia Antipolis, Facult\u00e9 de M\u00e9decine, Nice, France"},{"name":"Virologie, Centre Hospitalier Universitaire l'Archet, Nice, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Byumseok","family":"Koh","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Stanford University, Stanford, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shoujun","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Stanford University, Stanford, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Gong","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Stanford University, Stanford, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose G.","family":"Montoya","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Stanford University, Stanford, California, USA"},{"name":"Palo Alto Medical Foundation Toxoplasma Serology Laboratory, Palo Alto, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongjie","family":"Dai","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Stanford University, Stanford, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)16412-X"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1086\/590149"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.05013-11"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2014.09.018"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1086\/605433"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1701-2163(15)31053-7"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.108.2815.660"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1586\/14787210.2013.825441"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2010.07.011"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2008.07.005"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.4.1570-1574.2005"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2009.07.013"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2006.01564.x"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00089-13"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/142.5.757"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/pd.2052"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01663-15"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1258\/096914107780154440"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.4.878-884.1995"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.7.3481-3483.2005"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1097\/INF.0b013e3182343096"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3619"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms1477"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1039\/C4SC01206B"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1002\/smll.201502182"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.11.6.562-568.1980"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/162.1.270"},{"key":"e_1_3_3_29_2","first-page":"360","article-title":"Understanding interobserver agreement: the kappa statistic","volume":"37","author":"Viera AJ","year":"2005","unstructured":"Viera AJ, Garrett JM. 2005. Understanding interobserver agreement: the kappa statistic. Fam Med 37:360\u2013363.","journal-title":"Fam Med"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.2471\/BLT.12.111732"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001333"},{"key":"e_1_3_3_32_2","first-page":"929","article-title":"The past and present role of the Sabin-Feldman dye test in the serodiagnosis of toxoplasmosis","volume":"77","author":"Reiter-Owona I","year":"1999","unstructured":"Reiter-Owona I, Petersen E, Joynson D, Asp\u00f6ck H, Dard\u00e9 ML, Disko R, Dreazen O, Dumon H, Grillo R, Gross U, Hayde M, Holliman R, Ho-Yen DO, Janitschke K, Jenum PA, Naser K, Olszewski M, Thulliez P, Seitz HM. 1999. The past and present role of the Sabin-Feldman dye test in the serodiagnosis of toxoplasmosis. Bull World Health Organ 77:929\u2013935.","journal-title":"Bull World Health Organ"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1080\/10647440500148024"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-38-4-286"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.6.1450-1453.1997"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00624-13"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.03371-15","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.03371-15","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T09:46:43Z","timestamp":1646473603000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.03371-15"}},"subtitle":[],"editor":[{"given":"Y.-W.","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2016,7]]},"references-count":35,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2016,7]]}},"alternative-id":["10.1128\/JCM.03371-15"],"URL":"https:\/\/doi.org\/10.1128\/jcm.03371-15","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,7]]},"assertion":[{"value":"2016-01-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-03-16","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-06-24","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}