{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T01:17:20Z","timestamp":1784855840805,"version":"3.55.0"},"reference-count":12,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2015,6,1]],"date-time":"2015-06-01T00:00:00Z","timestamp":1433116800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2015,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The protozoan parasite\n            <jats:named-content content-type=\"genus-species\">Toxoplasma gondii<\/jats:named-content>\n            causes severe opportunistic infections. Here, we report an unexpected diagnosis of cerebral toxoplasmosis.\n            <jats:named-content content-type=\"genus-species\">T. gondii<\/jats:named-content>\n            was diagnosed by 16S and D2 large-subunit (LSU) ribosomal DNA (rDNA) sequencing of a cerebral biopsy specimen and confirmed by\n            <jats:named-content content-type=\"genus-species\">T. gondii<\/jats:named-content>\n            -specific PCR and immunohistochemistry. The patient was later diagnosed with HIV\/AIDS.\n          <\/jats:p>","DOI":"10.1128\/jcm.03404-14","type":"journal-article","created":{"date-parts":[[2015,4,9]],"date-time":"2015-04-09T02:41:28Z","timestamp":1428547288000},"page":"1983-1985","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":5,"title":["Unexpected Diagnosis of Cerebral Toxoplasmosis by 16S and D2 Large-Subunit Ribosomal DNA PCR and Sequencing"],"prefix":"10.1128","volume":"53","author":[{"given":"Alexandra Y. C.","family":"Kruse","sequence":"first","affiliation":[{"name":"Department of Clinical Microbiology, Copenhagen University Hospital-Rigshospitalet, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lasse","family":"Kvich","sequence":"additional","affiliation":[{"name":"Department of International Health, Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steffen","family":"Eickhardt","sequence":"additional","affiliation":[{"name":"Department of International Health, Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lars H.","family":"Omland","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Copenhagen University Hospital-Rigshospitalet, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Bjarnsholt","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Copenhagen University Hospital-Rigshospitalet, Copenhagen, Denmark"},{"name":"Department of International Health, Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claus","family":"Moser","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Copenhagen University Hospital-Rigshospitalet, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.03313-12"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-3-7"},{"key":"e_1_3_2_4_2","first-page":"89","volume-title":"Infectious diseases of the fetus and newborn infants","author":"Remington JS","year":"1990","unstructured":"Remington JS, Desmonts G. 1990. Toxoplasmosis, p 89\u2013195. In Remington JS, Klein JO (ed), Infectious diseases of the fetus and newborn infants. WB Saunders, Philadelphia, PA."},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pedhc.2010.04.008"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2009.04.003"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1469-0691.2002.00485.x"},{"key":"e_1_3_2_8_2","first-page":"211","article-title":"Toxoplasmosis in the adult","volume":"50","author":"Remington JS","year":"1974","unstructured":"Remington JS. 1974. Toxoplasmosis in the adult. Bull N Y Acad Med 50:211\u2013227.","journal-title":"Bull N Y Acad Med"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/15.2.211"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199212033272306"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/157.1.1"},{"key":"e_1_3_2_12_2","volume-title":"Guidelines for the prevention and treatment of opportunistic infections in HIV-infected adults and adolescents: Toxoplasma gondii encephalitis","author":"Panel on Opportunistic Infections in HIV-Infected Adults and Adolescents","year":"2015","unstructured":"Panel on Opportunistic Infections in HIV-Infected Adults and Adolescents. 16 April 2015, revision date. Guidelines for the prevention and treatment of opportunistic infections in HIV-infected adults and adolescents: Toxoplasma gondii encephalitis. National Institutes for Health, Bethesda, MD. http:\/\/aidsinfo.nih.gov\/guidelines\/html\/4\/adult-and-adolescent-oi-prevention-and-treatment-guidelines\/322\/toxo."},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(98)00096-4"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.03404-14","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.03404-14","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T12:13:09Z","timestamp":1646482389000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.03404-14"}},"subtitle":[],"editor":[{"given":"G. A.","family":"Land","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2015,6]]},"references-count":12,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2015,6]]}},"alternative-id":["10.1128\/JCM.03404-14"],"URL":"https:\/\/doi.org\/10.1128\/jcm.03404-14","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,6]]},"assertion":[{"value":"2014-12-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-04-03","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-05-14","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}