{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T19:22:04Z","timestamp":1791487324216,"version":"4.3.4"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2015,6,1]],"date-time":"2015-06-01T00:00:00Z","timestamp":1433116800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2015,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            While PCR is the most common method used for detecting\n            <jats:named-content content-type=\"genus-species\">Bordetella pertussis<\/jats:named-content>\n            in the United States, most laboratories use insertion sequence\n            <jats:italic>481<\/jats:italic>\n            (IS\n            <jats:italic>481<\/jats:italic>\n            ), which is not specific for\n            <jats:named-content content-type=\"genus-species\">B. pertussis<\/jats:named-content>\n            ; therefore, the relative contribution of other\n            <jats:named-content content-type=\"genus-species\">Bordetella<\/jats:named-content>\n            species is not understood. The objectives of this study were to evaluate the proportion of other\n            <jats:named-content content-type=\"genus-species\">Bordetella<\/jats:named-content>\n            species misidentified as\n            <jats:named-content content-type=\"genus-species\">B. pertussis<\/jats:named-content>\n            during a period of increased pertussis incidence, determine the level of agreement in\n            <jats:named-content content-type=\"genus-species\">Bordetella<\/jats:named-content>\n            species detection between U.S. commercial laboratories and the CDC, and assess the relative diagnostic sensitivity of CDC's PCR assay when using a different PCR master mix. Specimens collected between May 2012 and May 2013 were tested at two U.S. commercial laboratories for\n            <jats:named-content content-type=\"genus-species\">B. pertussis<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">B. parapertussis<\/jats:named-content>\n            detection. Every fifth specimen positive for IS\n            <jats:italic>481<\/jats:italic>\n            and\/or IS\n            <jats:italic>1001<\/jats:italic>\n            with cycle threshold (\n            <jats:italic>\n              C\n              <jats:sub>T<\/jats:sub>\n            <\/jats:italic>\n            ) values of \u226435 was sent to CDC for PCR testing that identifies\n            <jats:named-content content-type=\"genus-species\">Bordetella<\/jats:named-content>\n            species. Specimens with indeterminate or negative results in the CDC PCR were tested using an alternate PCR master mix. Of 755 specimens, there was agreement in species identification for 83.4% (\n            <jats:italic>n<\/jats:italic>\n            = 630). Of the specimens with different identifications (\n            <jats:italic>n<\/jats:italic>\n            = 125), 79.2% (\n            <jats:italic>n<\/jats:italic>\n            = 99) were identified as indeterminate\n            <jats:named-content content-type=\"genus-species\">B. pertussis<\/jats:named-content>\n            at CDC. Overall, 0.66% (\n            <jats:italic>n<\/jats:italic>\n            = 5) of the specimens were identified as\n            <jats:named-content content-type=\"genus-species\">B. holmesii<\/jats:named-content>\n            or\n            <jats:named-content content-type=\"genus-species\">B. bronchiseptica<\/jats:named-content>\n            at CDC. Of 115 specimens with indeterminate or negative results, 46.1% (\n            <jats:italic>n<\/jats:italic>\n            = 53) were\n            <jats:named-content content-type=\"genus-species\">B. pertussis<\/jats:named-content>\n            positive when tested by an alternate master mix, suggesting a possible increase in assay sensitivity. This study demonstrates good agreement between the two U.S. commercial laboratories and CDC and little misidentification of\n            <jats:named-content content-type=\"genus-species\">Bordetella<\/jats:named-content>\n            species during the 2012 U.S. epidemic.\n          <\/jats:p>","DOI":"10.1128\/jcm.03567-14","type":"journal-article","created":{"date-parts":[[2015,3,26]],"date-time":"2015-03-26T09:31:26Z","timestamp":1427362286000},"page":"1842-1847","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":18,"title":["Evaluation of Level of Agreement in Bordetella Species Identification in Three U.S. Laboratories during a Period of Increased Pertussis"],"prefix":"10.1128","volume":"53","author":[{"given":"Brunil\u00eds","family":"Burgos-Rivera","sequence":"first","affiliation":[{"name":"Centers for Disease Control and Prevention, U.S. Department of Health and Human Services, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adria D.","family":"Lee","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention, U.S. Department of Health and Human Services, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katherine E.","family":"Bowden","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention, U.S. Department of Health and Human Services, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda E.","family":"Faulkner","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention, U.S. Department of Health and Human Services, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brent L.","family":"Seaton","sequence":"additional","affiliation":[{"name":"Focus Diagnostics, Cypress, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bryndon D.","family":"Lembke","sequence":"additional","affiliation":[{"name":"Laboratory Corporation of America Holdings, Minnetonka, Minnesota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles P.","family":"Cartwright","sequence":"additional","affiliation":[{"name":"Laboratory Corporation of America Holdings, Minnetonka, Minnesota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stacey W.","family":"Martin","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention, U.S. Department of Health and Human Services, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. 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