{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T01:19:55Z","timestamp":1773710395211,"version":"3.50.1"},"reference-count":17,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2012,2,1]],"date-time":"2012-02-01T00:00:00Z","timestamp":1328054400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2012,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            We present an algorithm based on three PCR assays for\n            <jats:named-content content-type=\"genus-species\">Leishmania<\/jats:named-content>\n            (\n            <jats:named-content content-type=\"genus-species\">Viannia<\/jats:named-content>\n            ) species identification and assessed its performance using 70 specimens from Peruvian patients. The succession of the assayed targets can be ordered according to species prevalence. Sequential progression through the algorithm reduced the number of samples here studied by approximately 30% after each step.\n          <\/jats:p>","DOI":"10.1128\/jcm.05061-11","type":"journal-article","created":{"date-parts":[[2011,11,25]],"date-time":"2011-11-25T01:14:27Z","timestamp":1322183667000},"page":"495-498","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":24,"title":["Leishmania (Viannia) Species Identification on Clinical Samples from Cutaneous Leishmaniasis Patients in Peru: Assessment of a Molecular Stepwise Approach"],"prefix":"10.1128","volume":"50","author":[{"given":"Nicolas","family":"Veland","sequence":"first","affiliation":[{"name":"Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea K.","family":"Boggild","sequence":"additional","affiliation":[{"name":"Tropical Disease Unit, Division of Infectious Diseases, Toronto General Hospital, Toronto, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristian","family":"Valencia","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Braulio M.","family":"Valencia","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alejandro","family":"Llanos-Cuentas","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Peru"},{"name":"Hospital Nacional Cayetano Heredia, Lima, Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gert","family":"Van der Auwera","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Institute of Tropical Medicine Antwerp, Antwerp, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean-Claude","family":"Dujardin","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Institute of Tropical Medicine Antwerp, Antwerp, Belgium"},{"name":"Department of Biomedical Sciences, Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, Antwerp, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jorge","family":"Arevalo","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Peru"},{"name":"Departamento de Bioqu\u00edmica, Biolog\u00eda Molecular y Farmacolog\u00eda, Laboratorios de Investigaci\u00f3n y Desarrollo, Facultad de Ciencias y Filosof\u00eda, Universidad Peruana Cayetano Heredia, Lima, Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1086\/518041"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02457-10"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1086\/648730"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0102-311X2000000400013"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro981"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2009.11.007"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2004.07.003"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.trstmh.2006.06.009"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.5.2294-2297.2004"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1086\/524042"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1998.59.312"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2007.76.573"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00645-09"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2010.06.024"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.44.2.459-467.2006"},{"key":"e_1_3_3_17_2","volume-title":"World Health Organization technical report series no. 949.","author":"World Health Organization","year":"2010","unstructured":"World Health Organization. 2010. 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