{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T07:21:58Z","timestamp":1780384918708,"version":"3.54.1"},"reference-count":29,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2012,5,1]],"date-time":"2012-05-01T00:00:00Z","timestamp":1335830400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2012,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Effective skin antisepsis is of central importance in the prevention of wound infections, colonization of medical devices, and nosocomial transmission of microorganisms. Current antiseptics have a suboptimal efficacy resulting in substantial infectious morbidity, mortality, and increased health care costs. Here, we introduce an\n            <jats:italic>in vitro<\/jats:italic>\n            method for antiseptic testing and a novel alcohol-based antiseptic containing 4 to 5% of the polar aprotic solvent dimethyl sulfoxide (DMSO). The DMSO-containing antiseptic resulted in a 1- to 2-log enhanced killing of\n            <jats:named-content content-type=\"genus-species\">Staphylococcus epidermidis<\/jats:named-content>\n            and other microbes\n            <jats:italic>in vitro<\/jats:italic>\n            compared to the same antiseptic without DMSO. In a prospective clinical validation, blood culture contamination rates were reduced from 3.04% for 70% isopropanol\u20131% iodine (control antiseptic) to 1.04% for 70% isopropanol\u20131% iodine\u20135% DMSO (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.01). Our results predict that improved skin antisepsis is possible using new formulations of antiseptics containing strongly polarized but nonionizing (polar aprotic) solvents.\n          <\/jats:p>","DOI":"10.1128\/jcm.05106-11","type":"journal-article","created":{"date-parts":[[2012,3,1]],"date-time":"2012-03-01T05:57:08Z","timestamp":1330581428000},"page":"1552-1557","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":21,"title":["Dimethyl Sulfoxide Enhances Effectiveness of Skin Antiseptics and Reduces Contamination Rates of Blood Cultures"],"prefix":"10.1128","volume":"50","author":[{"given":"Jeffrey J.","family":"Tarrand","sequence":"first","affiliation":[{"name":"Departments of Laboratory Medicine, Infection Control, and Employee Health, The University of Texas M. D. Anderson Cancer Center, Houston, Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul R.","family":"LaSala","sequence":"additional","affiliation":[{"name":"Departments of Laboratory Medicine, Infection Control, and Employee Health, The University of Texas M. D. Anderson Cancer Center, Houston, Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang-Yang","family":"Han","sequence":"additional","affiliation":[{"name":"Departments of Laboratory Medicine, Infection Control, and Employee Health, The University of Texas M. D. Anderson Cancer Center, Houston, Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenneth V.","family":"Rolston","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Infection Control, and Employee Health, The University of Texas M. D. Anderson Cancer Center, Houston, Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dimitrios P.","family":"Kontoyiannis","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Infection Control, and Employee Health, The University of Texas M. D. Anderson Cancer Center, Houston, Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1991.03460030071031"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.5858\/2005-129-1222-TIBCCA"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1086\/502374"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0702.010216"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1067\/mic.2002.130391"},{"key":"e_1_3_2_7_2","unstructured":"Centers for Medicare and Medicaid Services. 14 March 2012 accession date. Quality of care measure specifications: surgical infection prevention. Centers for Medicare and Medicaid Services Baltimore MD. http:\/\/www.cms.hhs.gov\/hospitalqualityinits\/01_overview.asp."},{"key":"e_1_3_2_8_2","volume-title":"Statistics in medicine.","author":"Coltron T","year":"1974","unstructured":"ColtronT. 1974. Statistics in medicine. Little Brown & Co., Boston, MA."},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0039-6109(16)42031-1"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9343(91)90361-Z"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa0810988"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhin.2009.06.002"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/ajhp\/55.8.777"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1089\/10962960260496325"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02162-08"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.mi.44.100190.003051"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1097\/00000658-197703000-00002"},{"key":"e_1_3_2_18_2","first-page":"1","article-title":"2000 National hospital discharge survey","volume":"19","author":"Hall MJ","year":"2002","unstructured":"HallMJ OwingsMF. 2002. 2000 National hospital discharge survey. Adv. Data 19:1\u201318.","journal-title":"Adv. Data"},{"key":"e_1_3_2_19_2","first-page":"188","volume-title":"Zinsser microbiology","author":"Joklik WJ","year":"1992","unstructured":"JoklikWJ WillettHP. 1992. Sterilization and disinfection, p 188\u2013199. In JoklikWJ WillettHP AmosDB WilfertCM (ed), Zinsser microbiology, 20th ed. Appleton & Lange, Norwalk, CT.","edition":"20"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja063363t"},{"key":"e_1_3_2_21_2","unstructured":"StrandC\nShulmanJA. 1988. Bloodstream infections: laboratory detection and clinical considerations. ASCP Press Chicago IL."},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.29.10.2245-2249.1991"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.16.4.772-774.1982"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1086\/502073"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1089\/sur.2006.7.s1-19"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2011.10458.x"},{"key":"e_1_3_2_27_2","first-page":"194","volume-title":"Manual of clinical microbiology","author":"Weinstein M","year":"2007","unstructured":"WeinsteinM CarrollKC. 2007. Manual and automated systems for detection and identification of microorganisms, p 194. In MurrayPR (ed), Manual of clinical microbiology, 9th ed. ASM Press, Washington, DC.","edition":"9"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.6.2275-2278.2003"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/0041-008X(65)90081-5"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.2307\/30142416"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.05106-11","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.05106-11","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T11:20:00Z","timestamp":1646479200000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.05106-11"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,5]]},"references-count":29,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2012,5]]}},"alternative-id":["10.1128\/JCM.05106-11"],"URL":"https:\/\/doi.org\/10.1128\/jcm.05106-11","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,5]]},"assertion":[{"value":"2011-07-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-02-23","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-04-13","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}