{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T17:49:36Z","timestamp":1791308976433,"version":"4.3.1"},"reference-count":33,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2013,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Coagulase-negative staphylococci have been identified as major causes of late-onset neonatal bacteremia in neonatal intensive care units. Sixty isolates of\n            <jats:named-content content-type=\"genus-species\">Staphylococcus capitis<\/jats:named-content>\n            obtained from blood cultures of neonates between 2000 and 2005 were examined in this study. Biochemical analysis confirmed that 52 of these isolates belonged to the subsp.\n            <jats:named-content content-type=\"genus-species\">urealyticus<\/jats:named-content>\n            , and the remaining 8 belonged to the subsp.\n            <jats:named-content content-type=\"genus-species\">capitis.<\/jats:named-content>\n            Isolates of the predominant subsp.\n            <jats:named-content content-type=\"genus-species\">urealyticus<\/jats:named-content>\n            clones were characterized by their resistance to penicillin, erythromycin, and oxacillin and their biofilm formation ability, whereas subsp.\n            <jats:named-content content-type=\"genus-species\">capitis<\/jats:named-content>\n            isolates were generally antibiotic susceptible and biofilm negative. Pulsed-field gel electrophoresis (PFGE) after SacII digestion separated the 60 isolates into five major clusters. Sequence analysis showed that, in\n            <jats:named-content content-type=\"genus-species\">S. capitis<\/jats:named-content>\n            , the\n            <jats:italic>ica<\/jats:italic>\n            operon plus the negative regulator\n            <jats:italic>icaR<\/jats:italic>\n            was 4,160 bp in length. PCRs demonstrated the presence of the\n            <jats:italic>ica<\/jats:italic>\n            operon in all isolates. Further analysis of five isolates (two biofilm-positive subsp.\n            <jats:named-content content-type=\"genus-species\">urealyticus<\/jats:named-content>\n            , one biofilm-negative subsp.\n            <jats:named-content content-type=\"genus-species\">urealyticus<\/jats:named-content>\n            , and two biofilm-negative subsp.\n            <jats:named-content content-type=\"genus-species\">capitis<\/jats:named-content>\n            ) revealed that the\n            <jats:italic>ica<\/jats:italic>\n            operons were identical in all of the biofilm-positive subsp.\n            <jats:named-content content-type=\"genus-species\">urealyticus<\/jats:named-content>\n            strains; however, the biofilm-negative isolates showed variations. The distinctive phenotypic and genotypic characteristics revealed by this study may affect the epidemiology of the two subspecies of\n            <jats:named-content content-type=\"genus-species\">S. capitis<\/jats:named-content>\n            in the clinical setting. These results may provide a better understanding of the contribution of these two species to bloodstream infections in neonates.\n          <\/jats:p>","DOI":"10.1128\/jcm.05124-11","type":"journal-article","created":{"date-parts":[[2012,10,11]],"date-time":"2012-10-11T13:02:16Z","timestamp":1349960536000},"page":"9-14","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":62,"title":["Differences between Two Clinical Staphylococcus capitis Subspecies as Revealed by Biofilm, Antibiotic Resistance, and Pulsed-Field Gel Electrophoresis Profiling"],"prefix":"10.1128","volume":"51","author":[{"given":"Bintao","family":"Cui","sequence":"first","affiliation":[{"name":"School of Applied Sciences, RMIT University, Bundoora, Victoria, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter M.","family":"Smooker","sequence":"additional","affiliation":[{"name":"School of Applied Sciences, RMIT University, Bundoora, Victoria, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duncan A.","family":"Rouch","sequence":"additional","affiliation":[{"name":"School of Applied Sciences, RMIT University, Bundoora, Victoria, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew J.","family":"Daley","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Royal Children's Hospital, Parkville, Victoria, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margaret A.","family":"Deighton","sequence":"additional","affiliation":[{"name":"School of Applied Sciences, RMIT University, Bundoora, Victoria, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.9.4877-4879.2005"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1015665810739"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.9.5648-5655.2003"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.0030057"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.04515.x"},{"key":"e_1_3_2_7_2","first-page":"849","article-title":"Biofilm-associated proteins","volume":"329","author":"Latasa C","year":"2006","unstructured":"LatasaC SolanoC Penad\u00e9sJR LasaI. 2006. 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