{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T02:17:04Z","timestamp":1790043424159,"version":"4.0.1"},"reference-count":21,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2011,12,1]],"date-time":"2011-12-01T00:00:00Z","timestamp":1322697600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2011,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A multiplex PCR method was developed for the detection of\n            <jats:named-content content-type=\"genus-species\">Clostridium difficile<\/jats:named-content>\n            toxin genes\n            <jats:italic>tcdA<\/jats:italic>\n            ,\n            <jats:italic>tcdB<\/jats:italic>\n            ,\n            <jats:italic>ctdA<\/jats:italic>\n            , and\n            <jats:italic>cdtB<\/jats:italic>\n            and the major in-frame deletion types (18, 39, and 54 bp) of\n            <jats:italic>tcdC<\/jats:italic>\n            . The method has high specificity for PCR ribotype 027 and may identify other\n            <jats:italic>C<\/jats:italic>\n            .\n            <jats:italic>difficile<\/jats:italic>\n            strains of clinical and epidemiological importance.\n          <\/jats:p>","DOI":"10.1128\/jcm.05161-11","type":"journal-article","created":{"date-parts":[[2011,10,6]],"date-time":"2011-10-06T00:53:59Z","timestamp":1317862439000},"page":"4299-4300","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":72,"title":["Multiplex PCR Method for Detection of Clostridium difficile tcdA,\n            <i>tcdB<\/i>\n            ,\n            <i>cdtA<\/i>\n            , and\n            <i>cdtB<\/i>\n            and Internal In-Frame Deletion of\n            <i>tcdC<\/i>"],"prefix":"10.1128","volume":"49","author":[{"given":"S\u00f8ren","family":"Persson","sequence":"first","affiliation":[{"name":"Department of Microbiological Diagnostics, Unit of Gastrointestinal Infection, Statens Serum Institut, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joan N.","family":"Jensen","sequence":"additional","affiliation":[{"name":"Department of Microbiological Diagnostics, Unit of Gastrointestinal Infection, Statens Serum Institut, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katharina E. P.","family":"Olsen","sequence":"additional","affiliation":[{"name":"Department of Microbiological Diagnostics, Unit of Gastrointestinal Infection, Statens Serum Institut, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","first-page":"976","article-title":"Binary toxin and death after Clostridium difficile infection. Emerg","volume":"17","author":"Bacci S.","year":"2011","unstructured":"BacciS. MolbakK. KjeldsenM. K. OlsenK. E. 2011. Binary toxin and death after Clostridium difficile infection. Emerg. Infect. Dis. 17:976\u2013982.","journal-title":"Infect. 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M.","year":"2011","unstructured":"SoesL. M. 10 July 2011, posting date. Clinical features of Clostridium difficile infection and molecular characterization of the isolated strains in a cohort of Danish hospitalized patients. Eur. J. Clin. Microbiol. Infect. Dis. [Epub ahead of print.]","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"e_1_3_1_21_2","first-page":"24","article-title":"High prevalence of tcdC deletion-carrying Clostridium difficile and lack of association with disease severity. Diagn. Microbiol","volume":"66","author":"Verdoorn B. P.","year":"2010","unstructured":"VerdoornB. P. 2010. High prevalence of tcdC deletion-carrying Clostridium difficile and lack of association with disease severity. Diagn. Microbiol. Infect. Dis. 66:24\u201328.","journal-title":"Infect. 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