{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T16:02:16Z","timestamp":1790352136405,"version":"4.1.0"},"reference-count":53,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2012,3,1]],"date-time":"2012-03-01T00:00:00Z","timestamp":1330560000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2012,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Sequence-characterized amplified region (SCAR) markers, generated by randomly amplified polymorphic DNA (RAPD)-PCR, were developed to detect\n            <jats:named-content content-type=\"genus-species\">Histoplasma capsulatum<\/jats:named-content>\n            selectively in clinical and environmental samples. A 1,200-bp RAPD-PCR-specific band produced with the 1281-1283 primers was cloned, sequenced, and used to design two SCAR markers, 1281-1283\n            <jats:sub>220<\/jats:sub>\n            and 1281-1283\n            <jats:sub>230<\/jats:sub>\n            . The specificity of these markers was confirmed by Southern hybridization. To evaluate the relevance of the SCAR markers for the diagnosis of histoplasmosis, another molecular marker (M antigen probe) was used for comparison. To validate 1281-1283\n            <jats:sub>220<\/jats:sub>\n            and 1281-1283\n            <jats:sub>230<\/jats:sub>\n            as new tools for the identification of\n            <jats:named-content content-type=\"genus-species\">H. capsulatum<\/jats:named-content>\n            , the specificity and sensitivity of these markers were assessed for the detection of the pathogen in 36 clinical (17 humans, as well as 9 experimentally and 10 naturally infected nonhuman mammals) and 20 environmental (10 contaminated soil and 10 guano) samples. Although the two SCAR markers and the M antigen probe identified\n            <jats:named-content content-type=\"genus-species\">H. capsulatum<\/jats:named-content>\n            isolates from different geographic origins in America, the 1281-1283\n            <jats:sub>220<\/jats:sub>\n            SCAR marker was the most specific and detected the pathogen in all samples tested. In contrast, the 1281-1283\n            <jats:sub>230<\/jats:sub>\n            SCAR marker and the M antigen probe also amplified DNA from\n            <jats:named-content content-type=\"genus-species\">Aspergillus niger<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">Cryptococcus neoformans<\/jats:named-content>\n            , respectively. Both SCAR markers detected as little as 0.001 ng of\n            <jats:named-content content-type=\"genus-species\">H. capsulatum<\/jats:named-content>\n            DNA, while the M antigen probe detected 0.5 ng of fungal DNA. The SCAR markers revealed the fungal presence better than the M antigen probe in contaminated soil and guano samples. Based on our results, the 1281-1283\n            <jats:sub>220<\/jats:sub>\n            marker can be used to detect and identify\n            <jats:named-content content-type=\"genus-species\">H. capsulatum<\/jats:named-content>\n            in samples from different sources.\n          <\/jats:p>","DOI":"10.1128\/jcm.05271-11","type":"journal-article","created":{"date-parts":[[2011,12,22]],"date-time":"2011-12-22T02:24:53Z","timestamp":1324520693000},"page":"673-679","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":22,"title":["Development of Specific Sequence-Characterized Amplified Region Markers for Detecting Histoplasma capsulatum in Clinical and Environmental Samples"],"prefix":"10.1128","volume":"50","author":[{"given":"Mar\u00eda Guadalupe","family":"Fr\u00edas De Le\u00f3n","sequence":"first","affiliation":[{"name":"Departamentos de Microbiolog\u00eda-Parasitolog\u00eda, Facultad de Medicina, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Mexico City, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabina","family":"Arenas L\u00f3pez","sequence":"additional","affiliation":[{"name":"Fisiolog\u00eda, Facultad de Medicina, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Mexico City, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Lucia","family":"Taylor","sequence":"additional","affiliation":[{"name":"Departamentos de Microbiolog\u00eda-Parasitolog\u00eda, Facultad de Medicina, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Mexico City, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustavo","family":"Acosta Altamirano","sequence":"additional","affiliation":[{"name":"Hospital Ju\u00e1rez de M\u00e9xico, Mexico City, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda del Roc\u00edo","family":"Reyes-Montes","sequence":"additional","affiliation":[{"name":"Departamentos de Microbiolog\u00eda-Parasitolog\u00eda, Facultad de Medicina, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Mexico City, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.65.12.5421-5426.1999"},{"key":"e_1_3_2_3_2","first-page":"103","volume-title":"Histoplasmosis: proceedings of the second national conference.","author":"Ajello L","year":"1971","unstructured":"AjelloL. 1971. Distribution of Histoplasma capsulatum in the United States, p 103\u2013122. In AjelloL ChickEW FurcolowML (ed), Histoplasmosis: proceedings of the second national conference. Charles C Thomas Publishers, Springfield, IL."},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.5.1644-1647.2002"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.4.1753-1755.2003"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.3.1456-1458.2005"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-006-0207-y"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2011(02)00190-8"},{"key":"e_1_3_2_10_2","first-page":"187","article-title":"I. El murci\u00e9lago como reservorio y responsable de la dispersi\u00f3n de Histoplasma capsulatum en la naturaleza. II. Papel de los marcadores moleculares del hongo aislado de murci\u00e9lagos infectados","volume":"11","author":"Ch\u00e1vez-Tapia CB","year":"1998","unstructured":"Ch\u00e1vez-TapiaCB. 1998. I. El murci\u00e9lago como reservorio y responsable de la dispersi\u00f3n de Histoplasma capsulatum en la naturaleza. II. Papel de los marcadores moleculares del hongo aislado de murci\u00e9lagos infectados. Rev. Inst. Nal. Enf. Resp. Mex. 11:187\u2013191.","journal-title":"Rev. Inst. Nal. Enf. Resp. Mex."},{"key":"e_1_3_2_11_2","first-page":"2718","volume-title":"Principles and practice of infectious diseases","author":"Deepe GS","year":"2000","unstructured":"DeepeGSJr. 2000. Histoplasma capsulatum, p 2718\u20132732. In MandellGL BennettJE DolinR (ed), Principles and practice of infectious diseases, 5th ed, vol 2. Churchill Livingstone, Philadelphia, PA.","edition":"5"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1638\/2006-0062.1"},{"key":"e_1_3_2_13_2","first-page":"83","article-title":"Utilidad de las t\u00e9cnicas moleculares en el diagn\u00f3stico de la histoplasmosis","volume":"25","author":"Fr\u00edas De Le\u00f3n MG","year":"2007","unstructured":"Fr\u00edas De Le\u00f3nMG TaylorML Hern\u00e1ndez-Ram\u00edrezA Reyes-MontesMR. 2007. Utilidad de las t\u00e9cnicas moleculares en el diagn\u00f3stico de la histoplasmosis. Rev. Mex. Micol. 25:83\u201390.","journal-title":"Rev. Mex. Micol."},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.10.2618-2622.1997"},{"key":"e_1_3_2_15_2","first-page":"74","article-title":"Distribuci\u00f3n geogr\u00e1fica de la reactividad cut\u00e1nea a la histoplasmina en M\u00e9xico","volume":"31","author":"Gonz\u00e1lez-Ochoa A","year":"1971","unstructured":"Gonz\u00e1lez-OchoaA F\u00e9lixD. 1971. Distribuci\u00f3n geogr\u00e1fica de la reactividad cut\u00e1nea a la histoplasmina en M\u00e9xico. Rev. Invest. Salud Publ. Mex. 31:74\u201377.","journal-title":"Rev. Invest. Salud Publ. Mex."},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/17.10.4000"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1080\/02681219580000641"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1009637928471"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.4.6.346"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.31.2.419-421.1993"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.3.653-663.1999"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-294X.2003.01995.x"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.27.10.2369-2372.1989"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.10.4475-4480.2000"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0953756299008357"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.10.3505-3511.2001"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.3.1295-1298.2003"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.2.535-539.2003"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2007.03.008"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00332-09"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.32.4.867-870.1994"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1080\/02681219480000131"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00687-06"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1006\/mcpr.1999.0247"},{"key":"e_1_3_2_35_2","first-page":"195","article-title":"Tipificaci\u00f3n de aislados cl\u00ednicos de Histoplasma capsulatum por m\u00e9todos fenot\u00edpicos y genot\u00edpicos","volume":"11","author":"Reyes-Montes MR","year":"1998","unstructured":"Reyes-MontesMR. 1998. Tipificaci\u00f3n de aislados cl\u00ednicos de Histoplasma capsulatum por m\u00e9todos fenot\u00edpicos y genot\u00edpicos. Rev. Inst. Nal. Enf. Resp. M\u00e9x. 11:195\u2013201.","journal-title":"Rev. Inst. Nal. Enf. Resp. M\u00e9x."},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.5.1404-1408.1999"},{"key":"e_1_3_2_37_2","first-page":"102","article-title":"Identification of histoplasmosis infection source from two captive maras (Dolichotis patagonum) of the same colony by using molecular and immunologic assays","volume":"41","author":"Reyes-Montes MR","year":"2009","unstructured":"Reyes-MontesMR. 2009. Identification of histoplasmosis infection source from two captive maras (Dolichotis patagonum) of the same colony by using molecular and immunologic assays. Rev. Argent. Microbiol. 41:102\u2013104.","journal-title":"Rev. Argent. Microbiol."},{"key":"e_1_3_2_38_2","first-page":"1.25","volume-title":"Molecular cloning: a laboratory manual","author":"Sambrook J","year":"1989","unstructured":"SambrookJ FritschEF ManiatisT. 1989. Small-scale preparation of plasmid DNA: lysis by alkali, p 1.25\u20131.28. In FordN NolanC FergusonM (ed), Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, New York, NY.","edition":"2"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.11.2913-2919.1995"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1094\/Phyto-86-515"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.57.5.1409-1412.1989"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/49.suppl_1.11"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.31.4.845-850.1993"},{"key":"e_1_3_2_44_2","first-page":"46","article-title":"Urine polymerase chain reaction is not as sensitive as urine antigen for the diagnosis of disseminated histoplasmosis","volume":"53","author":"Tang YW","year":"2006","unstructured":"TangYW. 2006. Urine polymerase chain reaction is not as sensitive as urine antigen for the diagnosis of disseminated histoplasmosis. Diagn. Microbiol. Infect. Dis. 53:46\u201351.","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1006\/fgbi.2000.1219"},{"key":"e_1_3_2_46_2","unstructured":"TaylorML\nReyes-MontesMR. 2002. Nuevas aportaciones sobre la epidemiolog\u00eda de la histoplasmosis en M\u00e9xico: avances en el conocimiento de aspectos inmunol\u00f3gicos y moleculares y de la distribuci\u00f3n geogr\u00e1fica del agente etiol\u00f3gico. VITAE Academia Biomed. Digital CAIBO 10:1\u20133. http:\/\/caibco.ucv.ve\/vitae\/VitaeDiez\/Articulos\/Micologia\/Histoplasmosis\/ArchivosHTML\/Antecedentes.htm."},{"key":"e_1_3_2_47_2","first-page":"29","volume-title":"Research advances in microbiology.","author":"Taylor ML","year":"2000","unstructured":"TaylorML. 2000. Ecology and molecular epidemiology findings of Histoplasma capsulatum, in M\u00e9xico, p 29\u201335. In BenedikM (ed), Research advances in microbiology. Global Research Network, Kerala, India."},{"key":"e_1_3_2_48_2","first-page":"58","article-title":"Histoplasmosis en M\u00e9xico: aportaciones inmunol\u00f3gicas y moleculares sobre su epidemiolog\u00eda","volume":"23","author":"Taylor ML","year":"1997","unstructured":"TaylorML. 1997. Histoplasmosis en M\u00e9xico: aportaciones inmunol\u00f3gicas y moleculares sobre su epidemiolog\u00eda. Ciencia Desarrollo 23:58\u201363.","journal-title":"Ciencia Desarrollo"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsim.2005.05.017"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(03)00104-4"},{"key":"e_1_3_2_51_2","first-page":"208","article-title":"Histoplasmosis en M\u00e9xico, aspectos hist\u00f3ricos y epidemiol\u00f3gicos","volume":"11","author":"Vaca-Mar\u00edn MA","year":"1998","unstructured":"Vaca-Mar\u00ednMA Mart\u00ednez-RiveraMA Flores-EstradaJJ. 1998. Histoplasmosis en M\u00e9xico, aspectos hist\u00f3ricos y epidemiol\u00f3gicos. Rev. Inst. Nal. Enf. Resp. Mex. 11:208\u2013215.","journal-title":"Rev. Inst. Nal. Enf. Resp. Mex."},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0732-8893(02)00367-X"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198601093140205"},{"key":"e_1_3_2_54_2","first-page":"1205","article-title":"Diagnosis of histoplasmosis by antigen detection during an outbreak in Indianapolis","volume":"118","author":"Williams B","year":"1994","unstructured":"WilliamsB FojtasekM Connolly-StringfieldP WheatLJ. 1994. Diagnosis of histoplasmosis by antigen detection during an outbreak in Indianapolis. Arch. Pathol. Lab. Med. 118:1205\u20131208.","journal-title":"Arch. Pathol. Lab. Med."}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.05271-11","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.05271-11","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T11:22:01Z","timestamp":1646479321000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.05271-11"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,3]]},"references-count":53,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2012,3]]}},"alternative-id":["10.1128\/JCM.05271-11"],"URL":"https:\/\/doi.org\/10.1128\/jcm.05271-11","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,3]]},"assertion":[{"value":"2011-08-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-12-13","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-02-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}