{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T13:05:11Z","timestamp":1791119111063,"version":"4.1.0"},"reference-count":39,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2011,12,1]],"date-time":"2011-12-01T00:00:00Z","timestamp":1322697600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2011,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Using high-resolution melting (HRM) analysis, we developed a cost-effective method to genotype a set of 13 phylogenetically informative single-nucleotide polymorphisms (SNPs) within the genome of\n            <jats:named-content content-type=\"genus-species\">Bacillus anthracis<\/jats:named-content>\n            . SNP discrimination assays were performed in monoplex or duplex and applied to 100\n            <jats:named-content content-type=\"genus-species\">B. anthracis<\/jats:named-content>\n            isolates collected in France from 1953 to 2009 and a few reference strains. HRM provided a reliable and cheap alternative to subtype\n            <jats:named-content content-type=\"genus-species\">B. anthracis<\/jats:named-content>\n            into one of the 12 major sublineages or subgroups. All strains could be correctly positioned on the canonical SNP (canSNP) phylogenetic tree, except the divergent Pasteur vaccine strain ATCC 4229. We detected the cooccurrence of three canSNP subgroups in France. The dominant B.Br.CNEVA sublineage was found to be prevalent in the Alps, the Pyrenees, the Auvergne region, and the Sa\u00f4ne-et-Loire department. Strains affiliated with the A.Br.008\/009 subgroup were observed throughout most of the country. The minor A.Br.001\/002 subgroup was restricted to northeastern France. Multiple-locus variable-number tandem-repeat analysis using 24 markers further resolved French strains into 60 unique profiles and identified some regional patterns. Diversity found within the A.Br.008\/009 and B.Br.CNEVA subgroups suggests that these represent old, ecologically established clades in France. Phylogenetic relationships with strains from other parts of the world are discussed.\n          <\/jats:p>","DOI":"10.1128\/jcm.05439-11","type":"journal-article","created":{"date-parts":[[2011,10,13]],"date-time":"2011-10-13T05:30:44Z","timestamp":1318483844000},"page":"4286-4292","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":22,"title":["Characterization of Genetic Diversity of Bacillus anthracis in France by Using High-Resolution Melting Assays and Multilocus Variable-Number Tandem-Repeat Analysis"],"prefix":"10.1128","volume":"49","author":[{"given":"S.","family":"Derzelle","sequence":"first","affiliation":[{"name":"Anses, Animal Health Laboratory, Bacterial Zoonosis Unit, 94706 Maisons-Alfort, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Laroche","sequence":"additional","affiliation":[{"name":"Anses, Animal Health Laboratory, Bacterial Zoonosis Unit, 94706 Maisons-Alfort, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Le Fl\u00e8che","sequence":"additional","affiliation":[{"name":"University Paris-Sud, Institut de G\u00e9n\u00e9tique et Microbiologie, UMR 8621, Orsay F-91405, France"},{"name":"CNRS, Orsay F-91405, France"},{"name":"Division of Analytical Microbiology, DGA CBRN Defence, BP3, 91710 Vert le Petit, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Hauck","sequence":"additional","affiliation":[{"name":"University Paris-Sud, Institut de G\u00e9n\u00e9tique et Microbiologie, UMR 8621, Orsay F-91405, France"},{"name":"CNRS, Orsay F-91405, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Thierry","sequence":"additional","affiliation":[{"name":"Anses, Animal Health Laboratory, Bacterial Zoonosis Unit, 94706 Maisons-Alfort, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Vergnaud","sequence":"additional","affiliation":[{"name":"University Paris-Sud, Institut de G\u00e9n\u00e9tique et Microbiologie, UMR 8621, Orsay F-91405, France"},{"name":"CNRS, Orsay F-91405, France"},{"name":"DGA\/MRIS\u2014Mission pour la Recherche et l'Innovation Scientifique, 92221 Bagneux, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Madani","sequence":"additional","affiliation":[{"name":"Anses, Animal Health Laboratory, Bacterial Zoonosis Unit, 94706 Maisons-Alfort, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","first-page":"789","article-title":"Historical distribution and molecular diversity of Bacillus anthracis, Kazakhstan. 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