{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T16:04:00Z","timestamp":1791648240950,"version":"4.3.4"},"reference-count":35,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2012,2,1]],"date-time":"2012-02-01T00:00:00Z","timestamp":1328054400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2012,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            \u201cClassical\u201d Whipple's disease (cWD) is caused by\n            <jats:named-content content-type=\"genus-species\">Tropheryma whipplei<\/jats:named-content>\n            and is characterized by arthropathy, weight loss, and diarrhea.\n            <jats:named-content content-type=\"genus-species\">T. whipplei<\/jats:named-content>\n            infectious endocarditis (TWIE) is rarely reported, either in the context of cWD or as isolated TWIE without signs of systemic infection. The frequency of TWIE is unknown, and systematic studies are lacking. Here, we performed an observational cohort study on the incidence of\n            <jats:named-content content-type=\"genus-species\">T. whipplei<\/jats:named-content>\n            infection in explanted heart valves in two German university centers. Cardiac valves from 1,135 patients were analyzed for bacterial infection using conventional culture techniques, PCR amplification of the bacterial 16S rRNA gene, and subsequent sequencing.\n            <jats:named-content content-type=\"genus-species\">T. whipplei<\/jats:named-content>\n            -positive heart valves were confirmed by specific PCR, fluorescence\n            <jats:italic>in situ<\/jats:italic>\n            hybridization, immunohistochemistry, histological examination, and culture for\n            <jats:named-content content-type=\"genus-species\">T. whipplei<\/jats:named-content>\n            . Bacterial endocarditis was diagnosed in 255 patients, with streptococci, staphylococci, and enterococci being the main pathogens.\n            <jats:named-content content-type=\"genus-species\">T. whipplei<\/jats:named-content>\n            was the fourth most frequent pathogen, found in 16 (6.3%) cases, and clearly outnumbered\n            <jats:named-content content-type=\"genus-species\">Bartonella quintana<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">Coxiella burnetii<\/jats:named-content>\n            , and members of the HACEK group (\n            <jats:named-content content-type=\"genus-species\">Haemophilus<\/jats:named-content>\n            species,\n            <jats:named-content content-type=\"genus-species\">Actinobacillus actinomycetemcomitans<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">Cardiobacterium hominis<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">Eikenella corrodens<\/jats:named-content>\n            , and\n            <jats:named-content content-type=\"genus-species\">Kingella kingae<\/jats:named-content>\n            ). In this cohort,\n            <jats:named-content content-type=\"genus-species\">T. whipplei<\/jats:named-content>\n            was the most commonly found pathogen associated with culture-negative infective endocarditis.\n          <\/jats:p>","DOI":"10.1128\/jcm.05531-11","type":"journal-article","created":{"date-parts":[[2011,12,2]],"date-time":"2011-12-02T08:55:54Z","timestamp":1322816154000},"page":"216-222","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":130,"title":["High Frequency of Tropheryma whipplei in Culture-Negative Endocarditis"],"prefix":"10.1128","volume":"50","author":[{"given":"Walter","family":"Gei\u00dfd\u00f6rfer","sequence":"first","affiliation":[{"name":"Mikrobiologisches Institut-Klinische Mikrobiologie, Immunologie und Hygiene, Friedrich Alexander Universit\u00e4t Erlangen-N\u00fcrnberg und Universit\u00e4tsklinikum Erlangen, Erlangen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Verena","family":"Moos","sequence":"additional","affiliation":[{"name":"Medizinische Klinik I, Charit\u00e9-Universit\u00e4tsmedizin Berlin, CBF, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annette","family":"Moter","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Medizinische Mikrobiologie und Hygiene, Charit\u00e9-Universit\u00e4tsmedizin Berlin, CCM, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christoph","family":"Loddenkemper","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Pathologie, Charit\u00e9-Universit\u00e4tsmedizin Berlin, CBF, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Jansen","sequence":"additional","affiliation":[{"name":"Robert Koch Institut, Abteilung f\u00fcr Infektionsepidemiologie, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ren\u00e9","family":"Tandler","sequence":"additional","affiliation":[{"name":"Zentrum f\u00fcr Herzchirurgie, Universit\u00e4tsklinikum Erlangen, Erlangen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas J.","family":"Morguet","sequence":"additional","affiliation":[{"name":"Medizinische Klinik II, Kardiologie und Pulmologie, Charit\u00e9-Universit\u00e4tsmedizin Berlin, CBF, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florence","family":"Fenollar","sequence":"additional","affiliation":[{"name":"Unit\u00e9 des Rickettsies, URMITE CNRS-IRD UMR 6236, Facult\u00e9 de M\u00e9decine, Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Didier","family":"Raoult","sequence":"additional","affiliation":[{"name":"Unit\u00e9 des Rickettsies, URMITE CNRS-IRD UMR 6236, Facult\u00e9 de M\u00e9decine, Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Bogdan","sequence":"additional","affiliation":[{"name":"Mikrobiologisches Institut-Klinische Mikrobiologie, Immunologie und Hygiene, Friedrich Alexander Universit\u00e4t Erlangen-N\u00fcrnberg und Universit\u00e4tsklinikum Erlangen, Erlangen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Schneider","sequence":"additional","affiliation":[{"name":"Medizinische Klinik I, Charit\u00e9-Universit\u00e4tsmedizin Berlin, CBF, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"784","article-title":"Whipple's disease endocarditis: report of 5 cases and review of the literature","volume":"26","author":"Aiouaz H","year":"2005","unstructured":"AiouazH. 2005. Whipple's disease endocarditis: report of 5 cases and review of the literature. Rev. Med. Interne26:784\u2013790. (In French.)","journal-title":"Rev. Med. Interne"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1086\/375592"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ancard.2010.12.005"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.2006.00054.x"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2011.05.020"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.10.4487-4493.2004"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2009.03038.x"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1086\/322666"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1086\/528693"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2009.10.041"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1086\/653675"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/s100960100588"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/s15010-001-0135-9"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1136\/heart.89.3.263"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehp285"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.md.0000165658.82869.17"},{"key":"e_1_3_2_18_2","first-page":"213","article-title":"Aortic and mitral valve infective endocarditis caused by Tropheryma whipplei and with no gastrointestinal manifestations of Whipple's disease","volume":"13","author":"Kolek M","year":"2007","unstructured":"KolekMZaloudikovaBFreibergerTBratR. 2007. Aortic and mitral valve infective endocarditis caused by Tropheryma whipplei and with no gastrointestinal manifestations of Whipple's disease. Klin. Mikrobiol. Infekc. Lek. 13:213\u2013216. (In Czech.)","journal-title":"Klin. Mikrobiol. Infekc. 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A case of Tropheryma whipplei infective endocarditis of the aortic and mitral valves in association with psoriatic arthritis and lumbar discitis. J. Heart Valve Dis. 20:353\u2013356.","journal-title":"J. Heart Valve Dis."}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.05531-11","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.05531-11","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T06:02:05Z","timestamp":1646460125000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.05531-11"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,2]]},"references-count":35,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2012,2]]}},"alternative-id":["10.1128\/JCM.05531-11"],"URL":"https:\/\/doi.org\/10.1128\/jcm.05531-11","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,2]]},"assertion":[{"value":"2011-08-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-11-19","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-01-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}