{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T06:22:06Z","timestamp":1791354126569,"version":"4.3.1"},"reference-count":17,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2012,3,1]],"date-time":"2012-03-01T00:00:00Z","timestamp":1330560000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2012,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Babesiosis is an emerging zoonosis with important public health implications, as the incidence of the disease has risen dramatically over the past decade. Because the current gold standard for detection of\n            <jats:named-content content-type=\"genus-species\">Babesia<\/jats:named-content>\n            is microscopic examination of blood smears, accurate identification requires trained personnel. Species in the genus cannot be distinguished microscopically, and\n            <jats:named-content content-type=\"genus-species\">Babesia<\/jats:named-content>\n            can also be confused with the early trophozoite stage (ring forms) of\n            <jats:named-content content-type=\"genus-species\">Plasmodium<\/jats:named-content>\n            parasites. To allow more accurate diagnosis in a format that is accessible to a wider variety of laboratories, we developed a real-time PCR assay targeting the 18S rRNA gene of\n            <jats:named-content content-type=\"genus-species\">Babesia microti<\/jats:named-content>\n            , the dominant babesiosis pathogen in the United States. The real-time PCR is performed on DNA extracted from whole-blood specimens and detects\n            <jats:named-content content-type=\"genus-species\">Babesia microti<\/jats:named-content>\n            with a limit of detection of \u223c100 gene copies in 5 \u03bcl of blood. The real-time PCR assay was shown to be 100% specific when tested against a panel of 24 organisms consisting of\n            <jats:named-content content-type=\"genus-species\">Babesia microti<\/jats:named-content>\n            , other\n            <jats:named-content content-type=\"genus-species\">Babesia<\/jats:named-content>\n            species,\n            <jats:named-content content-type=\"genus-species\">Plasmodium<\/jats:named-content>\n            species, tick-borne and other pathogenic bacteria, and other blood-borne parasites. The results using clinical specimens show that the assay can detect infections of lower parasitemia than can be detected by microscopic examination. This method is therefore a rapid, sensitive, and accurate method for detection of\n            <jats:named-content content-type=\"genus-species\">Babesia microti<\/jats:named-content>\n            in patient specimens.\n          <\/jats:p>","DOI":"10.1128\/jcm.05848-11","type":"journal-article","created":{"date-parts":[[2011,12,15]],"date-time":"2011-12-15T04:57:46Z","timestamp":1323925066000},"page":"903-908","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":105,"title":["A New Real-Time PCR Assay for Improved Detection of the Parasite Babesia microti"],"prefix":"10.1128","volume":"50","author":[{"given":"Allen E.","family":"Teal","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases, Wadsworth Center, New York State Department of Health, Albany New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Habura","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Wadsworth Center, New York State Department of Health, Albany New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jill","family":"Ennis","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Wadsworth Center, New York State Department of Health, Albany New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janet S.","family":"Keithly","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Wadsworth Center, New York State Department of Health, Albany New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan","family":"Madison-Antenucci","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Wadsworth Center, New York State Department of Health, Albany New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"29","article-title":"Lyme disease\u2013United States, 2000","volume":"51","author":"Centers for Disease Control","year":"2002","unstructured":"Centers for Disease Control. 2002. 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