{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T11:46:13Z","timestamp":1777549573888,"version":"3.51.4"},"reference-count":45,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2012,3,1]],"date-time":"2012-03-01T00:00:00Z","timestamp":1330560000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2012,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The frequency of\n            <jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>\n            O157 genotypes among bovine, food, and human clinical isolates from The Netherlands was studied. Genotyping included the lineage-specific polymorphism assay (LSPA6), the Shiga-toxin-encoding bacteriophage insertion site assay (SBI), and PCR detection and\/or subtyping of virulence factors and markers [\n            <jats:italic>stx1<\/jats:italic>\n            ,\n            <jats:italic>stx<\/jats:italic>\n            <jats:sub>2a<\/jats:sub>\n            \/\n            <jats:italic>stx<\/jats:italic>\n            <jats:sub>2c<\/jats:sub>\n            ,\n            <jats:italic>q21<\/jats:italic>\n            \/\n            <jats:italic>Q933<\/jats:italic>\n            ,\n            <jats:italic>tir<\/jats:italic>\n            (\n            <jats:italic>A255T<\/jats:italic>\n            ), and\n            <jats:italic>rhsA<\/jats:italic>\n            (\n            <jats:italic>C3468G<\/jats:italic>\n            )]. LSPA6 lineage II dominated among bovine isolates (63%), followed by lineage I\/II (35.6%) and lineage I (1.4%). In contrast, the majority of the human isolates were typed as lineage I\/II (77.6%), followed by lineage I (14.1%) and lineage II (8.2%). Multivariate analysis revealed that the\n            <jats:italic>tir<\/jats:italic>\n            (\n            <jats:italic>A255T<\/jats:italic>\n            ) SNP and the\n            <jats:italic>\n              stx\n              <jats:sub>2a<\/jats:sub>\n              \/stx\n            <\/jats:italic>\n            <jats:sub>2c<\/jats:sub>\n            gene variants were the genetic features most differentiating human from bovine isolates. Bovine and food isolates were dominated by\n            <jats:italic>stx<\/jats:italic>\n            <jats:sub>2c<\/jats:sub>\n            (86.4% and 65.5%, respectively). Among human isolates, the frequency of\n            <jats:italic>stx<\/jats:italic>\n            <jats:sub>2c<\/jats:sub>\n            was 36.5%, while the frequencies of\n            <jats:italic>stx<\/jats:italic>\n            <jats:sub>2a<\/jats:sub>\n            and\n            <jats:italic>stx<\/jats:italic>\n            <jats:sub>2a<\/jats:sub>\n            plus\n            <jats:italic>stx<\/jats:italic>\n            <jats:sub>2c<\/jats:sub>\n            were 41.2% and 22.4%, respectively. Bovine isolates showed equal distribution of\n            <jats:italic>tir<\/jats:italic>\n            (255A) (54.8%) and\n            <jats:italic>tir<\/jats:italic>\n            (255T) (45.2%), while human isolates were dominated by the\n            <jats:italic>tir<\/jats:italic>\n            (255T) genotype (92.9%). LSPA6 lineage I isolates were all genotype\n            <jats:italic>stx<\/jats:italic>\n            <jats:sub>2c<\/jats:sub>\n            and\n            <jats:italic>tir<\/jats:italic>\n            (255T), while LSPA6 lineage II was dominated by\n            <jats:italic>tir<\/jats:italic>\n            (255A) (86.4%) and\n            <jats:italic>stx<\/jats:italic>\n            <jats:sub>2c<\/jats:sub>\n            (90.9%). LSPA6 lineage I\/II isolates were all genotype\n            <jats:italic>tir<\/jats:italic>\n            (255T) but showed more variation in\n            <jats:italic>stx<\/jats:italic>\n            <jats:sub>2<\/jats:sub>\n            types. The results support the hypothesis that in The Netherlands, the genotypes primarily associated with human disease form a minor subpopulation in the bovine reservoir. Comparison with published data revealed that the distribution of LSPA6 lineages among bovine and human clinical isolates differs considerably between The Netherlands and North America.\n          <\/jats:p>","DOI":"10.1128\/jcm.05964-11","type":"journal-article","created":{"date-parts":[[2011,12,22]],"date-time":"2011-12-22T02:24:53Z","timestamp":1324520693000},"page":"772-780","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":50,"title":["Genetic Features Differentiating Bovine, Food, and Human Isolates of Shiga Toxin-Producing Escherichia coli O157 in The Netherlands"],"prefix":"10.1128","volume":"50","author":[{"given":"Eelco","family":"Franz","sequence":"first","affiliation":[{"name":"National Institute for Public Health and the Environment (RIVM), Centre for Infectious Disease Control, Laboratory for Zoonoses and Environmental Microbiology, Bilthoven, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angela H. 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M.","family":"van Hoek","sequence":"additional","affiliation":[{"name":"National Institute for Public Health and the Environment (RIVM), Centre for Infectious Disease Control, Laboratory for Zoonoses and Environmental Microbiology, Bilthoven, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fimme J.","family":"van der Wal","sequence":"additional","affiliation":[{"name":"Central Veterinary Institute of Wageningen University &amp; Research Centre, Lelystad, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Albert","family":"de Boer","sequence":"additional","affiliation":[{"name":"Central Veterinary Institute of Wageningen University &amp; Research Centre, Lelystad, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ans","family":"Zwartkruis-Nahuis","sequence":"additional","affiliation":[{"name":"Food and Consumer Product Safety Authority, Zutphen, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kim","family":"van der Zwaluw","sequence":"additional","affiliation":[{"name":"National Institute for Public Health and the Environment, Centre for Infectious Disease Control, Laboratory for Infectious Diseases and Perinatal Screening, Bilthoven, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henk J. 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