{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,29]],"date-time":"2026-03-29T09:53:32Z","timestamp":1774778012067,"version":"3.50.1"},"reference-count":39,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2012,5,1]],"date-time":"2012-05-01T00:00:00Z","timestamp":1335830400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2012,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The worldwide dissemination of extended-spectrum-\u03b2-lactamase (ESBL)- and carbapenemase-producing\n            <jats:named-content content-type=\"genus-species\">Enterobacteriaceae<\/jats:named-content>\n            is a major concern in both hospital and community settings. Rapid identification of these resistant pathogens and the genetic determinants they possess is needed to assist in clinical practice and epidemiological studies. A collection of\n            <jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">Klebsiella pneumoniae<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">Klebsiella oxytoca<\/jats:named-content>\n            , and\n            <jats:named-content content-type=\"genus-species\">Proteus mirabilis<\/jats:named-content>\n            isolates, including phenotypically ESBL-positive (\n            <jats:italic>n<\/jats:italic>\n            = 1,093) and ESBL-negative isolates (\n            <jats:italic>n<\/jats:italic>\n            = 59), obtained in 2008\u20132009 from a longitudinal surveillance study (SMART) was examined using an\n            <jats:italic>in vitro<\/jats:italic>\n            nucleic acid-based microarray. This approach was used to detect and identify\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>ESBL<\/jats:sub>\n            (\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>SHV<\/jats:sub>\n            ,\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>TEM<\/jats:sub>\n            , and\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M<\/jats:sub>\n            genes of groups 1, 2, 9, and 8\/25) and\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>KPC<\/jats:sub>\n            genes and was combined with selective PCR amplification and DNA sequencing for complete characterization of the\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>ESBL<\/jats:sub>\n            and\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>KPC<\/jats:sub>\n            genes. Of the 1,093 phenotypically ESBL-positive isolates, 1,041 were identified as possessing at least one\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>ESBL<\/jats:sub>\n            gene (95.2% concordance), and 59 phenotypically ESBL-negative isolates, used as negative controls, were negative. Several ESBL variants of\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>TEM<\/jats:sub>\n            (\n            <jats:italic>n<\/jats:italic>\n            = 5),\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>SHV<\/jats:sub>\n            (\n            <jats:italic>n<\/jats:italic>\n            = 11),\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M<\/jats:sub>\n            (\n            <jats:italic>n<\/jats:italic>\n            = 19), and\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>KPC<\/jats:sub>\n            (\n            <jats:italic>n<\/jats:italic>\n            = 3) were detected. A new\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>SHV<\/jats:sub>\n            variant,\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>SHV-129<\/jats:sub>\n            , and a new\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>KPC<\/jats:sub>\n            variant,\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>KPC-11<\/jats:sub>\n            , were also identified. The most common\n            <jats:italic>bla<\/jats:italic>\n            genes found in this study were\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M-15<\/jats:sub>\n            ,\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M-14<\/jats:sub>\n            , and\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>SHV-12<\/jats:sub>\n            . Using nucleic acid microarrays, we obtained a \u201cmolecular snapshot\u201d of\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>ESBL<\/jats:sub>\n            genes in a current global population; we report that CTX-M-15 is still the dominant ESBL and provide the first report of the new \u03b2-lactamase variants\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>SHV-129<\/jats:sub>\n            and\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>KPC-11<\/jats:sub>\n            .\n          <\/jats:p>","DOI":"10.1128\/jcm.06115-11","type":"journal-article","created":{"date-parts":[[2012,2,9]],"date-time":"2012-02-09T02:43:51Z","timestamp":1328755431000},"page":"1632-1639","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":56,"title":["Using Nucleic Acid Microarrays To Perform Molecular Epidemiology and Detect Novel \u03b2-Lactamases: a Snapshot of Extended-Spectrum \u03b2-Lactamases throughout the World"],"prefix":"10.1128","volume":"50","author":[{"given":"Christine","family":"Lascols","sequence":"first","affiliation":[{"name":"International Health Management Associates, Schaumburg, Illinois, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meredith","family":"Hackel","sequence":"additional","affiliation":[{"name":"International Health Management Associates, Schaumburg, Illinois, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea M.","family":"Hujer","sequence":"additional","affiliation":[{"name":"Departments of Medicine, Case Western Reserve University School of Medicine, Cleveland, Ohio, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven H.","family":"Marshall","sequence":"additional","affiliation":[{"name":"Research Service, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, Ohio, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sam K.","family":"Bouchillon","sequence":"additional","affiliation":[{"name":"International Health Management Associates, Schaumburg, Illinois, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daryl J.","family":"Hoban","sequence":"additional","affiliation":[{"name":"International Health Management Associates, Schaumburg, Illinois, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen P.","family":"Hawser","sequence":"additional","affiliation":[{"name":"IHMA Europe S\u00e0rl, Epalinges, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert E.","family":"Badal","sequence":"additional","affiliation":[{"name":"International Health Management Associates, Schaumburg, Illinois, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert A.","family":"Bonomo","sequence":"additional","affiliation":[{"name":"Research Service, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, Ohio, USA"},{"name":"Departments of Medicine, Case Western Reserve University School of Medicine, Cleveland, Ohio, USA"},{"name":"Pharmacology, Case Western Reserve University School of Medicine, Cleveland, Ohio, USA"},{"name":"Molecular Biology and Microbiology, Case Western Reserve University School of Medicine, Cleveland, Ohio, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","first-page":"203","article-title":"Molecular characterisation by PCR-restriction fragment length polymorphism of TEM \u03b2-lactamases","volume":"134","author":"Arlet G","year":"1995","unstructured":"ArletG. 1995. 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