{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T15:03:21Z","timestamp":1791299001865,"version":"4.1.0"},"reference-count":33,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2012,4,1]],"date-time":"2012-04-01T00:00:00Z","timestamp":1333238400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2012,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Dissemination of carbapenem resistance among\n            <jats:named-content content-type=\"genus-species\">Enterobacteriaceae<\/jats:named-content>\n            poses a considerable threat to public health. Carbapenemase gene detection by molecular methods is the gold standard but is available in only a few laboratories. The aim of this study was to test phenotypic methods for the detection of metallo-\u03b2-lactamase (MBL)- or\n            <jats:named-content content-type=\"genus-species\">Klebsiella pneumoniae<\/jats:named-content>\n            carbapenemase (KPC)-producing\n            <jats:named-content content-type=\"genus-species\">Enterobacteriaceae<\/jats:named-content>\n            and associated mechanisms of \u03b2-lactam resistance against a panel of 30 genotypically characterized carbapenem-resistant\n            <jats:named-content content-type=\"genus-species\">Enterobacteriaceae<\/jats:named-content>\n            : 9 MBL, 7 KPC, 6 OXA-48, and 8 extended-spectrum \u03b2-lactamase (ESBL) or AmpC \u03b2-lactamases associated with decreased permeability. We used carbapenemase inhibitor-impregnated agar to test for carbapenem-resistant strains. Differences in the inhibition zone sizes of the meropenem, imipenem, ertapenem, and doripenem disks were measured between control and inhibitor (EDTA or phenylboronic acid [PBA] with or without cloxacillin)-impregnated Mueller-Hinton agar with a cutoff of 10 mm. All 9 MBL- and 7 KPC-producing\n            <jats:named-content content-type=\"genus-species\">Enterobacteriaceae<\/jats:named-content>\n            were identified from the differences in zone size in the presence and absence of specific inhibitors, regardless of the carbapenem MICs and including isolates with low-level resistance to carbapenems. We also detected their associated \u03b2-lactam resistance mechanisms (11 ESBL-type and 5 class A \u03b2-lactamase 2b). No differences in zone size were observed for OXA-48-producing strains or other carbapenem resistance mechanisms such as ESBL and decreased permeability. We propose a new strategy to detect carbapenemases (MBL- and KPC-type) and associated mechanisms of \u03b2-lactam resistance (ESBL or class A \u03b2-lactamase 2b) by the use of inhibitor-impregnated agar. A rapid phenotypic detection of resistance mechanisms is important for epidemiological purposes and for limiting the spread of resistant strains by implementing specific infection control measures.\n          <\/jats:p>","DOI":"10.1128\/jcm.06131-11","type":"journal-article","created":{"date-parts":[[2012,1,19]],"date-time":"2012-01-19T05:50:08Z","timestamp":1326952208000},"page":"1295-1302","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":89,"title":["Phenotypic Screening of Carbapenemases and Associated \u03b2-Lactamases in Carbapenem-Resistant Enterobacteriaceae"],"prefix":"10.1128","volume":"50","author":[{"given":"Andr\u00e9","family":"Birgy","sequence":"first","affiliation":[{"name":"Laboratoire de Microbiologie, H\u00f4pital Robert Debr\u00e9, Assistance Publique des H\u00f4pitaux de Paris, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Bidet","sequence":"additional","affiliation":[{"name":"Laboratoire de Microbiologie, H\u00f4pital Robert Debr\u00e9, Assistance Publique des H\u00f4pitaux de Paris, Paris, France"},{"name":"Universit\u00e9 Paris Diderot, Sorbonnes Paris Cit\u00e9, EA3105, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalie","family":"Genel","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Pierre et Marie Curie-Paris 6, Facult\u00e9 de M\u00e9decine, Site Saint-Antoine, Laboratoire de Bact\u00e9riologie, ER8, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Doit","sequence":"additional","affiliation":[{"name":"Laboratoire de Microbiologie, H\u00f4pital Robert Debr\u00e9, Assistance Publique des H\u00f4pitaux de Paris, Paris, France"},{"name":"Universit\u00e9 Paris Diderot, Sorbonnes Paris Cit\u00e9, EA3105, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominique","family":"Decr\u00e9","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Pierre et Marie Curie-Paris 6, Facult\u00e9 de M\u00e9decine, Site Saint-Antoine, Laboratoire de Bact\u00e9riologie, ER8, Paris, France"},{"name":"Laboratoire de Bact\u00e9riologie, H\u00f4pital Saint-Antoine, Assistance Publique des H\u00f4pitaux de Paris, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillaume","family":"Arlet","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Pierre et Marie Curie-Paris 6, Facult\u00e9 de M\u00e9decine, Site Saint-Antoine, Laboratoire de Bact\u00e9riologie, ER8, Paris, France"},{"name":"H\u00f4pital Tenon, Service de Bact\u00e9riologie-Hygi\u00e8ne, Assistance Publique des H\u00f4pitaux de Paris, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edouard","family":"Bingen","sequence":"additional","affiliation":[{"name":"Laboratoire de Microbiologie, H\u00f4pital Robert Debr\u00e9, Assistance Publique des H\u00f4pitaux de Paris, Paris, France"},{"name":"Universit\u00e9 Paris Diderot, Sorbonnes Paris Cit\u00e9, EA3105, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.1.40-43.2000"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2090229"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00540-11"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1001\/archinte.165.12.1430"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/46.6.895"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2009.03115.x"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkp431"},{"key":"e_1_3_2_9_2","first-page":"256","article-title":"Guidance for control of infections with carbapenem-resistant or carbapenemase-producing Enterobacteriaceae in acute care facilities","volume":"58","author":"Centers for Disease Control and Prevention","year":"2009","unstructured":"Centers for Disease Control and Prevention. 2009. 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