{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T02:51:43Z","timestamp":1791255103196,"version":"4.1.0"},"reference-count":49,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2012,3,1]],"date-time":"2012-03-01T00:00:00Z","timestamp":1330560000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2012,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The impact of Panton-Valentine leukocidin (PVL) on the outcome in\n            <jats:named-content content-type=\"genus-species\">Staphylococcus aureus<\/jats:named-content>\n            pneumonia is controversial. We genotyped\n            <jats:named-content content-type=\"genus-species\">S. aureus<\/jats:named-content>\n            isolates from patients with hospital-acquired pneumonia (HAP) enrolled in two registrational multinational clinical trials for the genetic elements carrying\n            <jats:italic>pvl<\/jats:italic>\n            and 30 other virulence genes. A total of 287 isolates (173 methicillin-resistant\n            <jats:named-content content-type=\"genus-species\">S. aureus<\/jats:named-content>\n            [MRSA] and 114 methicillin-susceptible\n            <jats:named-content content-type=\"genus-species\">S. aureus<\/jats:named-content>\n            [MSSA] isolates) from patients from 127 centers in 34 countries for whom clinical outcomes of cure or failure were available underwent genotyping. Of these,\n            <jats:italic>pvl<\/jats:italic>\n            was detected by PCR and its product confirmed in 23 isolates (8.0%) (MRSA, 18\/173 isolates [10.4%]; MSSA, 5\/114 isolates [4.4%]). The presence of\n            <jats:italic>pvl<\/jats:italic>\n            was not associated with a higher risk for clinical failure (4\/23 [17.4%] versus 48\/264 [18.2%];\n            <jats:italic>P<\/jats:italic>\n            = 1.00) or mortality. These findings persisted after adjustment for multiple potential confounding variables. No significant associations between clinical outcome and (i) presence of any of the 30 other virulence genes tested, (ii) presence of specific bacterial clone, (iii) levels of alpha-hemolysin, or (iv) delta-hemolysin production were identified. This study suggests that neither\n            <jats:italic>pvl<\/jats:italic>\n            presence nor\n            <jats:italic>in vitro<\/jats:italic>\n            level of alpha-hemolysin production is the primary determinant of outcome among patients with HAP caused by\n            <jats:named-content content-type=\"genus-species\">S. aureus<\/jats:named-content>\n            .\n          <\/jats:p>","DOI":"10.1128\/jcm.06219-11","type":"journal-article","created":{"date-parts":[[2011,12,29]],"date-time":"2011-12-29T02:57:26Z","timestamp":1325127446000},"page":"848-856","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":37,"title":["Presence of Genes Encoding Panton-Valentine Leukocidin Is Not the Primary Determinant of Outcome in Patients with Hospital-Acquired Pneumonia Due to Staphylococcus aureus"],"prefix":"10.1128","volume":"50","author":[{"given":"Batu K.","family":"Sharma-Kuinkel","sequence":"first","affiliation":[{"name":"Department of Medicine, Duke University Medical Center, Durham, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sun H.","family":"Ahn","sequence":"additional","affiliation":[{"name":"Department of Medicine, Duke University Medical Center, Durham, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas H.","family":"Rude","sequence":"additional","affiliation":[{"name":"Department of Medicine, Duke University Medical Center, Durham, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yurong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Medicine, Duke University Medical Center, Durham, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven Y. C.","family":"Tong","sequence":"additional","affiliation":[{"name":"Department of Medicine, Duke University Medical Center, Durham, North Carolina, USA"},{"name":"Menzies School of Health Research, Darwin, Northern Territory, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felicia","family":"Ruffin","sequence":"additional","affiliation":[{"name":"Department of Medicine, Duke University Medical Center, Durham, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fredric C.","family":"Genter","sequence":"additional","affiliation":[{"name":"Theravance, Inc., South San Francisco, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin R.","family":"Braughton","sequence":"additional","affiliation":[{"name":"Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank R.","family":"DeLeo","sequence":"additional","affiliation":[{"name":"Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven L.","family":"Barriere","sequence":"additional","affiliation":[{"name":"Theravance, Inc., South San Francisco, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr.","given":"Vance G.","family":"Fowler","sequence":"additional","affiliation":[{"name":"Department of Medicine, Duke University Medical Center, Durham, North Carolina, USA"},{"name":"Duke Clinical Research Institute, Durham, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01643-09"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm1207-1405"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1086\/592053"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01313-06"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01822-07"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1097\/INF.0b013e31821618be"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2010-2053"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-011-1203-4"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.3810\/hp.2010.02.274"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0912403107"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.3.1008-1015.2000"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9343(98)00151-X"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1086\/427148"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.180.14.3724-3726.1998"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(02)07877-7"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1086\/519263"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2010.02.005"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1086\/523581"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/172.2.410"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jir441"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1086\/499632"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1137165"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2011.04.010"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1142\/S0192415X11009123"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02235-10"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-011-1316-9"},{"key":"e_1_3_2_28_2","first-page":"869","article-title":"Identification and characterization of Panton-Valentine leukocidin-positive Staphylococcus aureus isolated in Okinawa, Japan","volume":"58","author":"Miyagi A","year":"2010","unstructured":"MiyagiA. 2010. 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