{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T17:53:59Z","timestamp":1790790839449,"version":"4.1.0"},"reference-count":29,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2012,4,1]],"date-time":"2012-04-01T00:00:00Z","timestamp":1333238400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2012,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Mitochondrial DNA sequences offer major advantages over the more usual nuclear targets for loop-mediated isothermal amplification approaches (mito-LAMP) because multiple copies occur in every cell. Four LAMP primers [F3, FIP(F1c+F2), BIP(B1c+B2), and B3] were designed based on the mitochondrial\n                    <jats:italic>nad1<\/jats:italic>\n                    sequence of\n                    <jats:named-content content-type=\"genus-species\">Opisthorchis viverrini<\/jats:named-content>\n                    and used for a highly specific assay (mito-OvLAMP) to distinguish DNA of\n                    <jats:named-content content-type=\"genus-species\">O. viverrini<\/jats:named-content>\n                    from that of another opisthorchiid (\n                    <jats:named-content content-type=\"genus-species\">Clonorchis sinensis<\/jats:named-content>\n                    ) and other trematodes (\n                    <jats:named-content content-type=\"genus-species\">Haplorchis pumilio<\/jats:named-content>\n                    ,\n                    <jats:named-content content-type=\"genus-species\">Haplorchis taichui<\/jats:named-content>\n                    ,\n                    <jats:named-content content-type=\"genus-species\">Fasciola hepatica<\/jats:named-content>\n                    , and\n                    <jats:named-content content-type=\"genus-species\">Fasciola gigantica<\/jats:named-content>\n                    ). Conventional PCR was applied using F3\/B3 primer pairs to verify the specificity of the primers for\n                    <jats:named-content content-type=\"genus-species\">O. viverrini<\/jats:named-content>\n                    DNA templates. All LAMP-positive samples could be detected with the naked eye in sunlight, by gel electrophoresis (stained with ethidium bromide), and by addition of SYBR green I to the product in sunlight or under UV light. Only DNA from\n                    <jats:named-content content-type=\"genus-species\">O. viverrini<\/jats:named-content>\n                    yielded amplification products by LAMP (and by PCR verification), and the LAMP limit of detection was as little as 100 fg (10\n                    <jats:sup>\u22124<\/jats:sup>\n                    ng DNA), indicating that this assay is 10 to 100 times more sensitive than PCR. Field testing was done using representative egg and metacercarial samples collected from localities where the fluke is endemic. With the advantages of simplicity, rapidity, sensitivity, and cost effectiveness, mito-OvLAMP is a good tool for molecular detection and epidemiology studies in regions or countries where\n                    <jats:named-content content-type=\"genus-species\">O. viverrini<\/jats:named-content>\n                    is endemic, which can lead to more effective control of opisthorchiasis and trematodiasis.\n                  <\/jats:p>","DOI":"10.1128\/jcm.06277-11","type":"journal-article","created":{"date-parts":[[2012,2,8]],"date-time":"2012-02-08T21:43:51Z","timestamp":1328737431000},"page":"1178-1184","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":66,"title":["Development of Mitochondrial Loop-Mediated Isothermal Amplification for Detection of the Small Liver Fluke Opisthorchis viverrini (Opisthorchiidae; Trematoda; Platyhelminthes)"],"prefix":"10.1128","volume":"50","author":[{"given":"Thanh Hoa","family":"Le","sequence":"first","affiliation":[{"name":"Department of Immunology, Institute of Biotechnology, Vietnam Academy of Science and Technology (VAST), Cau Giay District, Hanoi, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nga Thi Bich","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Department of Immunology, Institute of Biotechnology, Vietnam Academy of Science and Technology (VAST), Cau Giay District, Hanoi, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nam Hai","family":"Truong","sequence":"additional","affiliation":[{"name":"Department of Genetic Engineering, Institute of Biotechnology, VAST, Cau Giay District, Hanoi, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nguyen Van","family":"De","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Hanoi Medical University, Dong Da District, Hanoi, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2010.09.005"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2011.08.009"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.idc.2010.07.006"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-010-1812-3"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.3347\/kjp.2009.47.S.S69"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-010-2162-x"},{"key":"e_1_3_2_8_2","first-page":"12","article-title":"The food-borne trematode zoonoses of Vietnam. 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