{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T00:30:51Z","timestamp":1791592251563,"version":"4.3.4"},"reference-count":48,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2012,3,1]],"date-time":"2012-03-01T00:00:00Z","timestamp":1330560000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2012,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Sequence type 22 (ST22) methicillin-resistant<jats:named-content content-type=\"genus-species\">Staphylococcus aureus<\/jats:named-content>(MRSA) harboring staphylococcal cassette chromosome<jats:italic>mec<\/jats:italic>(SCC<jats:italic>mec<\/jats:italic>) IV (ST22-MRSA-IV) has predominated in Irish hospitals since the late 1990s. Six distinct clones of community-associated MRSA (CA-MRSA) have also been identified in Ireland. A new strain of CA-MRSA, ST772-MRSA-V, has recently emerged and become widespread in India and has spread into hospitals. In the present study, highly similar MRSA isolates were recovered from seven colonized neonates in a neonatal intensive care unit (NICU) in a maternity hospital in Ireland during 2010 and 2011, two colonized NICU staff, one of their colonized children, and a NICU environmental site. The isolates exhibited multiantibiotic resistance,<jats:italic>spa<\/jats:italic>type t657, and were assigned to ST772-MRSA-V by DNA microarray profiling. All isolates encoded resistance to macrolides [<jats:italic>msr<\/jats:italic>(A) and<jats:italic>mpb<\/jats:italic>(BM)] and aminoglycosides (<jats:italic>aacA-aphD<\/jats:italic>and<jats:italic>aphA3<\/jats:italic>) and harbored the Panton-Valentine leukocidin toxin genes (<jats:italic>lukF-PV<\/jats:italic>and<jats:italic>lukS-PV<\/jats:italic>), enterotoxin genes (<jats:italic>sea<\/jats:italic>,<jats:italic>sec<\/jats:italic>,<jats:italic>sel<\/jats:italic>, and<jats:italic>egc<\/jats:italic>), and one of the immune evasion complex genes (<jats:italic>scn<\/jats:italic>). One of the NICU staff colonized by ST772-MRSA-V was identified as the probable index case, based on recent travel to India. Seven additional hospital and CA-ST772-MRSA-V isolates recovered from skin and soft tissue infections in Ireland between 2009 and 2011 exhibiting highly similar phenotypic and genotypic characteristics to the NICU isolates were also identified. The clinical details of four of these patients revealed connections with India through ethnic background or travel. Our study indicates that hospital-acquired and CA-ST772-MRSA-V is currently emerging in Ireland and may have been imported from India on several occasions.<\/jats:p>","DOI":"10.1128\/jcm.06354-11","type":"journal-article","created":{"date-parts":[[2011,12,22]],"date-time":"2011-12-22T02:24:53Z","timestamp":1324520693000},"page":"841-847","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":63,"title":["Emergence of Hospital- and Community-Associated Panton-Valentine Leukocidin-Positive Methicillin-Resistant Staphylococcus aureus Genotype ST772-MRSA-V in Ireland and Detailed Investigation of an ST772-MRSA-V Cluster in a Neonatal Intensive Care Unit"],"prefix":"10.1128","volume":"50","author":[{"given":"Gr\u00e1inne I.","family":"Brennan","sequence":"first","affiliation":[{"name":"National MRSA Reference Laboratory, St. James's Hospital, Dublin, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna C.","family":"Shore","sequence":"additional","affiliation":[{"name":"Microbiology Research Unit, Division of Oral Biosciences, Dublin Dental University Hospital, University of Dublin, Trinity College, Dublin, Ireland"},{"name":"Department of Clinical Microbiology, School of Medicine, University of Dublin, Trinity College, St. James's Hospital, Dublin, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suzanne","family":"Corcoran","sequence":"additional","affiliation":[{"name":"Microbiology Department, Rotunda Hospital, Dublin, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Tecklenborg","sequence":"additional","affiliation":[{"name":"Microbiology Research Unit, Division of Oral Biosciences, Dublin Dental University Hospital, University of Dublin, Trinity College, Dublin, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David C.","family":"Coleman","sequence":"additional","affiliation":[{"name":"Microbiology Research Unit, Division of Oral Biosciences, Dublin Dental University Hospital, University of Dublin, Trinity College, Dublin, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian","family":"O'Connell","sequence":"additional","affiliation":[{"name":"National MRSA Reference Laboratory, St. James's Hospital, Dublin, Ireland"},{"name":"Department of Clinical Microbiology, School of Medicine, University of Dublin, Trinity College, St. James's Hospital, Dublin, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"393","DOI":"10.7883\/yoken.JJID.2008.393","article-title":"Genetic characterization of Staphylococcus aureus isolates carrying Panton-Valentine leukocidin genes in Bangladesh","volume":"61","author":"Afroz S","year":"2008","unstructured":"AfrozS. 2008. 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