{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T13:39:55Z","timestamp":1762004395857},"reference-count":20,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2012,6,1]],"date-time":"2012-06-01T00:00:00Z","timestamp":1338508800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2012,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The emerging fungal pathogens\n            <jats:named-content content-type=\"genus-species\">Candida haemulonii<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">Candida pseudohaemulonii<\/jats:named-content>\n            often show high-level resistance to amphotericin B (AMB). We compared the utilities of five antifungal susceptibility testing methods, i.e., the Etest using Mueller-Hinton agar supplemented with glucose and methylene blue (Etest-MH), the Etest using RPMI agar supplemented with glucose (Etest-RPG), the Vitek-2 yeast susceptibility system, and the Clinical and Laboratory Standards Institute (CLSI) and European Committee on Antimicrobial Susceptibility Testing (EUCAST) broth microdilution methods, for the detection of AMB-resistant isolates of\n            <jats:named-content content-type=\"genus-species\">C. haemulonii<\/jats:named-content>\n            and closely related species. Thirty-eight clinical isolates (8\n            <jats:named-content content-type=\"genus-species\">C. haemulonii<\/jats:named-content>\n            , 10\n            <jats:named-content content-type=\"genus-species\">C. pseudohaemulonii<\/jats:named-content>\n            , and 20\n            <jats:named-content content-type=\"genus-species\">Candida auris<\/jats:named-content>\n            isolates) were analyzed. Of the 18\n            <jats:named-content content-type=\"genus-species\">C. haemulonii<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">C. pseudohaemulonii<\/jats:named-content>\n            isolates, 18, 15, 18, 10, and 9 exhibited AMB MICs of &gt;1 \u03bcg\/ml by the Etest-MH, Etest-RPG, Vitek-2, CLSI, and EUCAST methods, respectively. All 20\n            <jats:named-content content-type=\"genus-species\">C. auris<\/jats:named-content>\n            isolates showed AMB MICs of \u22641 \u03bcg\/ml by all five methods. Of the methods, the Etest-MH generated the broadest distribution of AMB MICs for all 38 isolates and showed the best discrimination between the\n            <jats:named-content content-type=\"genus-species\">C. haemulonii<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">C. pseudohaemulonii<\/jats:named-content>\n            isolates (4 to 32 \u03bcg\/ml) and those of\n            <jats:named-content content-type=\"genus-species\">C. auris<\/jats:named-content>\n            (0.125 to 0.5 \u03bcg\/ml). Taking the Etest-MH as the reference method, the essential agreements (within two dilutions) for the Etest-RPG, Vitek-2, CLSI, and EUCAST methods were 84, 92, 55, and 55%, respectively; the categorical agreements were 92, 92, 79, and 76%, respectively. This study provides the first data on the efficacy of the Etest-MH and its excellent agreement with Vitek-2 for discriminating AMB-resistant from AMB-susceptible isolates of these\n            <jats:named-content content-type=\"genus-species\">Candida<\/jats:named-content>\n            species.\n          <\/jats:p>","DOI":"10.1128\/jcm.06440-11","type":"journal-article","created":{"date-parts":[[2012,3,23]],"date-time":"2012-03-23T03:29:56Z","timestamp":1332473396000},"page":"1852-1855","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":55,"title":["Detection of Amphotericin B Resistance in Candida haemulonii and Closely Related Species by Use of the Etest, Vitek-2 Yeast Susceptibility System, and CLSI and EUCAST Broth Microdilution Methods"],"prefix":"10.1128","volume":"50","author":[{"given":"Jong Hee","family":"Shin","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, Chonnam National University Medical School, Gwangju, South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mi-Na","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, University of Ulsan College of Medicine and Asan Medical Center, Seoul, South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sook Jin","family":"Jang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Chosun University Medical School, Gwangju, South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min Young","family":"Ju","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Chonnam National University Medical School, Gwangju, South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soo Hyun","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Chonnam National University Medical School, Gwangju, South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Myung Geun","family":"Shin","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Chonnam National University Medical School, Gwangju, South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soon Pal","family":"Suh","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Chonnam National University Medical School, Gwangju, South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dong Wook","family":"Ryang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Chonnam National University Medical School, Gwangju, South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01840-06"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1080\/13693780701436794"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01096-09"},{"key":"e_1_3_2_5_2","unstructured":"Clinical and Laboratory Standards Institute. 2008. 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