{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T12:20:50Z","timestamp":1772626850718,"version":"3.50.1"},"reference-count":28,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2012,4,1]],"date-time":"2012-04-01T00:00:00Z","timestamp":1333238400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2012,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            We present a statistical summary of results from the Model Performance Evaluation Program (MPEP) for\n            <jats:named-content content-type=\"genus-species\">Mycobacterium tuberculosis<\/jats:named-content>\n            Drug Susceptibility Testing, 1994 to 2008, implemented by the U.S. Centers for Disease Control and Prevention (CDC). During that period, a total of 57,733 test results for culture isolates were reported by 216 participating laboratories for the first-line antituberculosis drugs used in the United States\u2014isoniazid (INH), rifampin (RMP), ethambutol (EMB), and pyrazinamide (PZA). Using Clinical Laboratory and Standards Institute (CLSI)-recommended concentrations for one or more of three methods, agar proportion (AP), BACTEC460 (Bactec), and MGIT-960 (MGIT), yielded overall agreement of 97.0% for first-line drugs. For susceptible strains, agreement was 98.4%; for resistant strains, agreement was 91.0%, with significantly lower accuracy (chi-square test,\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.0001). For resistant strains, overall agreement by methods was 91.3% for AP, 93.0% for Bactec, and 82.6% for MGIT and by drugs was 92.2% for INH, 91.5% for RMP, 79.0% for EMB, and 97.5% for PZA. For some strains, performance by method varied significantly. Use of duplicate strains in the same shipment and repeat strains over time revealed consistent performance even for strains with higher levels of interlaboratory discordance. No overall differences in performance between laboratories were observed based on volume of testing or type of facility (e.g., health department, hospital, independent). By all methods, decreased performance was observed for strains with low-level INH resistance, RMP resistance, and EMB-resistant strains. These results demonstrate a high level of performance in detection of drug-resistant\n            <jats:named-content content-type=\"genus-species\">M. tuberculosis<\/jats:named-content>\n            in U.S. laboratories.\n          <\/jats:p>","DOI":"10.1128\/jcm.06479-11","type":"journal-article","created":{"date-parts":[[2012,2,2]],"date-time":"2012-02-02T19:55:26Z","timestamp":1328212526000},"page":"1233-1239","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":26,"title":["Performance of Tuberculosis Drug Susceptibility Testing in U.S. Laboratories from 1994 to 2008"],"prefix":"10.1128","volume":"50","author":[{"given":"Pawan K.","family":"Angra","sequence":"first","affiliation":[{"name":"Laboratory Systems Development Branch, Division of Preparedness and Emerging Infections, National Center for Emerging and Zoonotic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas H.","family":"Taylor","sequence":"additional","affiliation":[{"name":"Division of Bacterial Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael F.","family":"Iademarco","sequence":"additional","affiliation":[{"name":"Laboratory Branch, Division of Tuberculosis Elimination, National Center for HIV\/AIDS, Viral Hepatitis, STD, and Tuberculosis Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beverly","family":"Metchock","sequence":"additional","affiliation":[{"name":"Laboratory Branch, Division of Tuberculosis Elimination, National Center for HIV\/AIDS, Viral Hepatitis, STD, and Tuberculosis Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. Rex","family":"Astles","sequence":"additional","affiliation":[{"name":"Laboratory Science, Policy and Practice Program Office, Office of Surveillance, Epidemiology &amp; Laboratory Services, Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John C.","family":"Ridderhof","sequence":"additional","affiliation":[{"name":"Laboratory Science, Policy and Practice Program Office, Office of Surveillance, Epidemiology &amp; Laboratory Services, Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2011.03551.x"},{"key":"e_1_3_2_3_2","first-page":"565","article-title":"Mycobacteria: laboratory methods for testing drug sensitivity and resistance","volume":"29","author":"Canetti G","year":"1963","unstructured":"CanettiG . 1963. 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