{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,5]],"date-time":"2022-04-05T06:34:27Z","timestamp":1649140467138},"reference-count":9,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2012,4,1]],"date-time":"2012-04-01T00:00:00Z","timestamp":1333238400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2012,4]]},"abstract":"ABSTRACT<\/jats:title>\n \n Chlamydia trachomatis<\/jats:named-content>\n and\n Neisseria gonorrhoeae<\/jats:named-content>\n are common causes of sexually transmitted infections, and there is interest in screening SurePath liquid-based Pap (L-Pap) samples with Aptima Combo 2 (AC2), Amplicor (AMP), and ProbeTec ET (PT) assays. SurePath L-Pap samples and a cervical swab (CS) were collected from 394 women attending health clinics in Hamilton and Toronto, ON, Canada. L-Pap samples were tested with the three assays prior to being processed for cytology, and the CS sample was tested with AC2. The prevalence of\n C. trachomatis<\/jats:named-content>\n was 8.9%, and that of\n N. gonorrhoeae<\/jats:named-content>\n was 1.5%. By using the positives from CS testing, as well as CS negatives corresponding to L-Pap samples that tested positive in 2 of 3 assays, the sensitivities of AC2, AMP, and PT for\n C. trachomatis<\/jats:named-content>\n in precytology samples were calculated to be 97.1% (34 of 35 positive samples were detected), 91.4% (32 of 35 were detected), and 77.1% (27 of 35 were detected), respectively. Six women were infected with\n N. gonorrhoeae<\/jats:named-content>\n . After cytology processing, the results of testing the remaining liquid in the L-Pap vial and the cell-enriched fraction for\n C. trachomatis<\/jats:named-content>\n by AC2 showed positive agreements of 98.9% (kappa [\n k<\/jats:italic>\n ], 0.93) and 98.7% (\n k<\/jats:italic>\n , 0.92), respectively, with the results of testing precytology L-Pap samples. Although all testing showed high specificity, testing for\n C. trachomatis<\/jats:named-content>\n by AC2 was significantly more sensitive than testing by PT for SurePath samples (\n P<\/jats:italic>\n = 0.02). Newer versions of AMP (Cobas 4800) and PT (Q\n \n x<\/jats:italic>\n <\/jats:sup>\n with XTR technology) need published evaluations for detecting\n C. trachomatis<\/jats:named-content>\n and\n N. gonorrhoeae<\/jats:named-content>\n in L-Pap samples.\n C. trachomatis<\/jats:named-content>\n testing can be performed with similar results on pre- and postcytology SurePath samples.\n <\/jats:p>","DOI":"10.1128\/jcm.06595-11","type":"journal-article","created":{"date-parts":[[2012,1,19]],"date-time":"2012-01-19T05:50:08Z","timestamp":1326952208000},"page":"1281-1284","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":4,"title":["Comparison of Three Assays for Detection of Chlamydia trachomatis and Neisseria gonorrhoeae in SurePath Pap Samples and the Role of Pre- and Postcytology Testing"],"prefix":"10.1128","volume":"50","author":[{"given":"M.","family":"Chernesky","sequence":"first","affiliation":[{"name":"St. Joseph's Healthcare\/McMaster University, Hamilton, ON, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Jang","sequence":"additional","affiliation":[{"name":"St. Joseph's Healthcare\/McMaster University, Hamilton, ON, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Portillo","sequence":"additional","affiliation":[{"name":"St. Joseph's Healthcare\/McMaster University, Hamilton, ON, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Smieja","sequence":"additional","affiliation":[{"name":"St. Joseph's Healthcare\/McMaster University, Hamilton, ON, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Kapala","sequence":"additional","affiliation":[{"name":"Gamma-Dynacare Medical Laboratories, Brampton, ON, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Doucette","sequence":"additional","affiliation":[{"name":"Gamma-Dynacare Medical Laboratories, Brampton, ON, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Sumner","sequence":"additional","affiliation":[{"name":"Gamma-Dynacare Medical Laboratories, Brampton, ON, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Ewert","sequence":"additional","affiliation":[{"name":"Evergreen Health Centre, Toronto, ON, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"MacRitchie","sequence":"additional","affiliation":[{"name":"Hamilton Community Health Centre, Hamilton, ON, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Gilchrist","sequence":"additional","affiliation":[{"name":"St. Joseph's Healthcare\/McMaster University, Hamilton, ON, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","volume-title":"Sexually transmitted disease surveillance, 2008","author":"Centers for Disease Control and Prevention","year":"2010","unstructured":"Centers for Disease Control and Prevention. 2010. Sexually transmitted disease surveillance, 2008. Centers for Disease Control and Prevention, Atlanta, GA."},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00405-07"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.44.2.400-405.2006"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0b013e3181a4c3fc"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00013-07"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.44.3.1084-1086.2006"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.4103\/1742-6413.65057"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.3.1372-1373.2005"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199605233342103"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.06595-11","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.06595-11","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T11:13:12Z","timestamp":1646478792000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.06595-11"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,4]]},"references-count":9,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2012,4]]}},"alternative-id":["10.1128\/JCM.06595-11"],"URL":"https:\/\/doi.org\/10.1128\/jcm.06595-11","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,4]]},"assertion":[{"value":"2011-12-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-01-10","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-03-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}