{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,8]],"date-time":"2026-02-08T19:31:53Z","timestamp":1770579113719,"version":"3.49.0"},"reference-count":37,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2012,4,1]],"date-time":"2012-04-01T00:00:00Z","timestamp":1333238400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2012,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The identification and detection of mitis group streptococci, which contain\n            <jats:named-content content-type=\"genus-species\">Streptococcus pneumoniae<\/jats:named-content>\n            , have been hampered by the lack of sensitive and specific assays. In this study, we evaluated several biochemical and molecular assays for the identification of\n            <jats:named-content content-type=\"genus-species\">S. pneumoniae<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">Streptococcus pseudopneumoniae<\/jats:named-content>\n            and their distinction from other mitis group streptococci using a collection of 54 isolates obtained by the routine culturing of 53 respiratory specimens from patients with community-acquired pneumonia. The combined results of the biochemical and molecular assays indicated the presence of 23\n            <jats:named-content content-type=\"genus-species\">S. pneumoniae<\/jats:named-content>\n            , 2\n            <jats:named-content content-type=\"genus-species\">S. pseudopneumoniae<\/jats:named-content>\n            , and 29 other mitis group streptococcal isolates. The tube bile solubility test that is considered gold standard for the identification of\n            <jats:named-content content-type=\"genus-species\">S. pneumoniae<\/jats:named-content>\n            showed concordant results with optochin susceptibility testing (CO\n            <jats:sub>2<\/jats:sub>\n            atmosphere) and a real-time multiplex PCR assay targeting the Spn9802 fragment and the autolysin gene. Optochin susceptibility testing upon incubation in an O\n            <jats:sub>2<\/jats:sub>\n            atmosphere, bile solubility testing by oxgall disk, matrix-assisted laser desorption ionization\u2013time of flight mass spectrometry, and sequence analysis of the\n            <jats:italic>tuf<\/jats:italic>\n            and\n            <jats:italic>rpoB<\/jats:italic>\n            genes resulted in several false-positive, false-negative, or inconclusive results. The\n            <jats:named-content content-type=\"genus-species\">S. pseudopneumoniae<\/jats:named-content>\n            isolates could be identified only by molecular assays, and the multiplex real-time PCR assay was concluded to be most convenient for the identification of\n            <jats:named-content content-type=\"genus-species\">S. pneumoniae<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">S. pseudopneumoniae<\/jats:named-content>\n            isolates. Using this method,\n            <jats:named-content content-type=\"genus-species\">S. pneumoniae<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">S. pseudopneumoniae<\/jats:named-content>\n            DNA could be detected in the respiratory samples from which they were isolated and in an additional 11 samples from which only other streptococci were isolated.\n          <\/jats:p>","DOI":"10.1128\/jcm.06609-11","type":"journal-article","created":{"date-parts":[[2012,1,26]],"date-time":"2012-01-26T06:16:02Z","timestamp":1327558562000},"page":"1171-1177","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":59,"title":["Evaluation of Several Biochemical and Molecular Techniques for Identification of Streptococcus pneumoniae and Streptococcus pseudopneumoniae and Their Detection in Respiratory Samples"],"prefix":"10.1128","volume":"50","author":[{"given":"Els","family":"Wessels","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology, Leiden University Medical Center, Leiden, Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacqueline J. G.","family":"Schelfaut","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Leiden University Medical Center, Leiden, Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandra T.","family":"Bernards","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Leiden University Medical Center, Leiden, Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric C. J.","family":"Claas","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Leiden University Medical Center, Leiden, Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2009.02714.x"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2009.03069.x"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-10-310"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2007.08.010"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.10.4686-4696.2004"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1186\/1741-7007-7-3"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02498-06"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.6.2651-2657.2004"},{"key":"e_1_3_3_10_2","volume-title":"CLSI document MM18-P","author":"CLSI","year":"2009","unstructured":"CLSI. 2009. 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