{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T03:22:59Z","timestamp":1792984979977,"version":"4.5.0"},"reference-count":26,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2012,5,1]],"date-time":"2012-05-01T00:00:00Z","timestamp":1335830400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2012,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The genus\n                    <jats:named-content content-type=\"genus-species\">Bartonella<\/jats:named-content>\n                    includes numerous species with varied host associations, including several that infect humans. Development of a molecular diagnostic method capable of detecting the diverse repertoire of\n                    <jats:named-content content-type=\"genus-species\">Bartonella<\/jats:named-content>\n                    species while maintaining genus specificity has been a challenge. We developed a novel real-time PCR assay targeting a 301-bp region of the\n                    <jats:italic>ssrA<\/jats:italic>\n                    gene of\n                    <jats:named-content content-type=\"genus-species\">Bartonella<\/jats:named-content>\n                    and demonstrated specific amplification in over 30\n                    <jats:named-content content-type=\"genus-species\">Bartonella<\/jats:named-content>\n                    species, subspecies, and strains. Subsequent analysis of\n                    <jats:italic>ssrA<\/jats:italic>\n                    sequences was sufficient to discriminate\n                    <jats:named-content content-type=\"genus-species\">Bartonella<\/jats:named-content>\n                    species and provided phylogenetic data consistent with that of\n                    <jats:italic>gltA<\/jats:italic>\n                    , a commonly used gene for differentiating\n                    <jats:named-content content-type=\"genus-species\">Bartonella<\/jats:named-content>\n                    genotypes. Using this assay, we identified\n                    <jats:named-content content-type=\"genus-species\">Bartonella<\/jats:named-content>\n                    DNA in 29% and 47% of blood specimens from elk in Wyoming and cattle in the Republic of Georgia, respectively. Sequence analysis of a subset of genotypes from elk specimens revealed a cluster most closely related to\n                    <jats:named-content content-type=\"genus-species\">Bartonella capreoli<\/jats:named-content>\n                    , and genotypes from cattle were identified as\n                    <jats:named-content content-type=\"genus-species\">Bartonella bovis<\/jats:named-content>\n                    , both\n                    <jats:named-content content-type=\"genus-species\">Bartonella<\/jats:named-content>\n                    species commonly found in wild and domestic ruminants. Considering the widespread geographic distribution and infectivity potential to a variety of hosts, this assay may be an effective diagnostic method for identification of\n                    <jats:named-content content-type=\"genus-species\">Bartonella<\/jats:named-content>\n                    infections in humans and have utility in\n                    <jats:named-content content-type=\"genus-species\">Bartonella<\/jats:named-content>\n                    surveillance studies.\n                  <\/jats:p>","DOI":"10.1128\/jcm.06621-11","type":"journal-article","created":{"date-parts":[[2012,3,1]],"date-time":"2012-03-01T00:57:08Z","timestamp":1330563428000},"page":"1645-1649","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":149,"title":["Development of a Novel Genus-Specific Real-Time PCR Assay for Detection and Differentiation of Bartonella Species and Genotypes"],"prefix":"10.1128","volume":"50","author":[{"given":"Maureen H.","family":"Diaz","sequence":"first","affiliation":[{"name":"Respiratory Diseases Branch, Division of Bacterial Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Bai","sequence":"additional","affiliation":[{"name":"Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lile","family":"Malania","sequence":"additional","affiliation":[{"name":"Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado, USA"},{"name":"National Center for Disease Control and Public Health, Tbilisi, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonas M.","family":"Winchell","sequence":"additional","affiliation":[{"name":"Respiratory Diseases Branch, Division of Bacterial Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael Y.","family":"Kosoy","sequence":"additional","affiliation":[{"name":"Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2008.02177.x"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.8.3462-3468.2004"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2010.09.022"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10493-009-9255-1"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.13.3.428"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0603.000313"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.2460\/javma.2004.224.1270"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.34.8.1952-1956.1996"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1374.080"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcp.2004.04.004"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00470-10"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1407.071110"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-51-4-1557"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.6.1327-1331.1997"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/jmedent\/33.3.490"},{"key":"e_1_3_2_17_2","first-page":"8","article-title":"Natural history of Bartonella infections (an exception to Koch's postulate)","volume":"9","author":"Jacomo V","year":"2002","unstructured":"JacomoV KellyPJ RaoultD. 2002. Natural history of Bartonella infections (an exception to Koch's postulate). Clin. Diagn. Lab. Immunol. 9:8\u201318.","journal-title":"Clin. Diagn. Lab. Immunol."},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/75843"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.4142\/jvs.2005.6.4.327"},{"key":"e_1_3_2_20_2","first-page":"1","article-title":"Bartonella infections","volume":"11","author":"Koehler JE","year":"1996","unstructured":"KoehlerJE. 1996. Bartonella infections. Adv. Pediatr. Infect. Dis. 11:1\u201327.","journal-title":"Adv. Pediatr. Infect. 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