{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T15:30:44Z","timestamp":1787671844144,"version":"build-2736575974"},"reference-count":9,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2012,5,1]],"date-time":"2012-05-01T00:00:00Z","timestamp":1335830400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2012,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Deep sequencing detected a potential bioterrorism agent,\n            <jats:named-content content-type=\"genus-species\">Francisella tularensis<\/jats:named-content>\n            , in a human abscess sample (Iwaki-08) of unknown etiology. Identified single-nucleotide variations suggest that the Iwaki-08 case was associated with\n            <jats:named-content content-type=\"genus-species\">Francisella tularensis<\/jats:named-content>\n            subsp.\n            <jats:named-content content-type=\"genus-species\">holarctica<\/jats:named-content>\n            (biovar\n            <jats:named-content content-type=\"genus-species\">japonica<\/jats:named-content>\n            ) but not the highly virulent type A (\n            <jats:named-content content-type=\"genus-species\">Francisella tularensis<\/jats:named-content>\n            subsp.\n            <jats:named-content content-type=\"genus-species\">tularensis<\/jats:named-content>\n            ).\n          <\/jats:p>","DOI":"10.1128\/jcm.06715-11","type":"journal-article","created":{"date-parts":[[2012,2,16]],"date-time":"2012-02-16T04:05:49Z","timestamp":1329365149000},"page":"1810-1812","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":36,"title":["Detection of a Possible Bioterrorism Agent, Francisella sp., in a Clinical Specimen by Use of Next-Generation Direct DNA Sequencing"],"prefix":"10.1128","volume":"50","author":[{"given":"Makoto","family":"Kuroda","sequence":"first","affiliation":[{"name":"Laboratory of Bacterial Genomics, Pathogen Genomics Center, National Institute of Infectious Diseases, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tsuyoshi","family":"Sekizuka","sequence":"additional","affiliation":[{"name":"Laboratory of Bacterial Genomics, Pathogen Genomics Center, National Institute of Infectious Diseases, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fumiaki","family":"Shinya","sequence":"additional","affiliation":[{"name":"Surgery, Iwaki Kyoritsu General Hospital, Iwaki, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fumihiko","family":"Takeuchi","sequence":"additional","affiliation":[{"name":"Laboratory of Bacterial Genomics, Pathogen Genomics Center, National Institute of Infectious Diseases, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takayuki","family":"Kanno","sequence":"additional","affiliation":[{"name":"Department of Pathology, National Institute of Infectious Diseases, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tetsutaro","family":"Sata","sequence":"additional","affiliation":[{"name":"Department of Pathology, National Institute of Infectious Diseases, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigeyuki","family":"Asano","sequence":"additional","affiliation":[{"name":"Departments of Pathology, Iwaki Kyoritsu General Hospital, Iwaki, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"13293","article-title":"Possession, use, and transfer of select agents and toxins","volume":"70","author":"CDC\/HHS","year":"2005","unstructured":"CDC\/HHS. 2005. Possession, use, and transfer of select agents and toxins. Final rule. Fed. Regist. 70:13293\u201313325.","journal-title":"Final rule. Fed. Regist."},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000459"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2009.07.017"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1868"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp698"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0019838"},{"key":"e_1_3_2_8_2","first-page":"291","article-title":"Infraspecific taxonomy of tularemia agent Francisella tularensis McCoy et Chapin","volume":"26","author":"Olsufjev NG","year":"1982","unstructured":"OlsufjevNG MeshcheryakovaIS. 1982. Infraspecific taxonomy of tularemia agent Francisella tularensis McCoy et Chapin. J. Hyg. Epidemiol. Microbiol. Immunol. 26:291\u2013299.","journal-title":"J. Hyg. Epidemiol. Microbiol. Immunol."},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bti191"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01786-08"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.06715-11","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.06715-11","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T11:20:44Z","timestamp":1646479244000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.06715-11"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,5]]},"references-count":9,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2012,5]]}},"alternative-id":["10.1128\/JCM.06715-11"],"URL":"https:\/\/doi.org\/10.1128\/jcm.06715-11","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,5]]},"assertion":[{"value":"2011-12-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-01-31","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-04-13","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}