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Assays could detect as few as 10 copies of negative-sense RNA and could be used in detecting low levels of HEV replication in cells. Virus particles in stool samples of hepatitis E patients showed encapsidation of negative-sense RNA along with HEV genomic RNA.<\/jats:p>","DOI":"10.1128\/jcm.06717-11","type":"journal-article","created":{"date-parts":[[2011,12,29]],"date-time":"2011-12-29T02:57:26Z","timestamp":1325127446000},"page":"1467-1470","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":23,"title":["Detection of Negative-Sense RNA in Packaged Hepatitis E Virions by Use of an Improved Strand-Specific Reverse Transcription-PCR Method"],"prefix":"10.1128","volume":"50","author":[{"given":"Subhashis N.","family":"Chatterjee","sequence":"first","affiliation":[{"name":"Hepatitis Division, National Institute of Virology, Pashan, Pune, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pradip B.","family":"Devhare","sequence":"additional","affiliation":[{"name":"Hepatitis Division, National Institute of Virology, Pashan, Pune, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kavita S.","family":"Lole","sequence":"additional","affiliation":[{"name":"Hepatitis Division, National Institute of Virology, Pashan, Pune, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2008.11.097"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2893.2006.00801.x"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00536-08"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01475-10"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00892-06"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.251555098"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01219-07"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00046-06"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01310568"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02259-06"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2893.2010.01351.x"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/s007050050384"},{"key":"e_1_3_1_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.1890420306"},{"key":"e_1_3_1_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.5.2430-2437.2000"},{"key":"e_1_3_1_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-0934(03)00218-0"},{"key":"e_1_3_1_17_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1996.0001"},{"key":"e_1_3_1_18_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.80705-0"},{"key":"e_1_3_1_19_2","doi-asserted-by":"publisher","DOI":"10.2144\/01301bm02"},{"key":"e_1_3_1_20_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.027714-0"},{"key":"e_1_3_1_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2011.03.028"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.06717-11","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.06717-11","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T11:12:07Z","timestamp":1646478727000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.06717-11"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,4]]},"references-count":20,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2012,4]]}},"alternative-id":["10.1128\/JCM.06717-11"],"URL":"https:\/\/doi.org\/10.1128\/jcm.06717-11","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,4]]},"assertion":[{"value":"2011-12-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-12-23","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-03-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}