{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T14:17:36Z","timestamp":1773670656232,"version":"3.50.1"},"reference-count":45,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2012,6,1]],"date-time":"2012-06-01T00:00:00Z","timestamp":1338508800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2012,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A set of 300 Dutch\n            <jats:named-content content-type=\"genus-species\">Cryptococcus neoformans<\/jats:named-content>\n            isolates, obtained from 237 patients during 1977 to 2007, was investigated by determining the mating type, serotype, and AFLP and microsatellite genotype and susceptibility to seven antifungal compounds. Almost half of the studied cases were from HIV-infected patients, followed by a patient group of individuals with other underlying diseases and immunocompetent individuals. The majority of the isolates were mating type \u03b1 and serotype A, followed by \u03b1D isolates and other minor categories. The most frequently observed genotype was AFLP1, distantly followed by AFLP2 and AFLP3. Microsatellite typing revealed a high genetic diversity among serotype A isolates but a lower diversity within the serotype D set of isolates. One patient was infected by multiple AFLP genotypes. Fluconazole and flucytosine had the highest geometric mean MICs of 2.9 and 3.5 \u03bcg\/ml, respectively, while amphotericin B (0.24 \u03bcg\/ml), itraconazole (0.08 \u03bcg\/ml), voriconazole (0.07 \u03bcg\/ml), posaconazole (0.06 \u03bcg\/ml), and isavuconazole (0.03 \u03bcg\/ml) had much lower geometric mean MICs. One isolate had a high flucytosine MIC (&gt;64 \u03bcg\/ml), while decreased susceptibility (\u226516 \u03bcg\/ml) for flucytosine and fluconazole was found in 9 and 10\n            <jats:named-content content-type=\"genus-species\">C. neoformans<\/jats:named-content>\n            isolates, respectively.\n          <\/jats:p>","DOI":"10.1128\/jcm.06750-11","type":"journal-article","created":{"date-parts":[[2012,3,23]],"date-time":"2012-03-23T03:29:56Z","timestamp":1332473396000},"page":"1918-1926","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":60,"title":["Extensive Genetic Diversity within the Dutch Clinical Cryptococcus neoformans Population"],"prefix":"10.1128","volume":"50","author":[{"given":"Ferry","family":"Hagen","sequence":"first","affiliation":[{"name":"Department of Yeast and Basidiomycete Research, CBS-KNAW Fungal Biodiversity Centre, Utrecht, The Netherlands"},{"name":"Department of Internal Medicine and Infectious Diseases, University Medical Centre Utrecht, Utrecht, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda-Teresa","family":"Illnait-Zaragoz\u00ed","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Infectious Diseases, Canisius Wilhelmina Hospital, Nijmegen, The Netherlands"},{"name":"Tropical Medicine Institute Pedro Kouri, Havana, Cuba"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacques F.","family":"Meis","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Infectious Diseases, Canisius Wilhelmina Hospital, Nijmegen, The Netherlands"},{"name":"Department of Medical Microbiology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William H. M.","family":"Chew","sequence":"additional","affiliation":[{"name":"Department of Yeast and Basidiomycete Research, CBS-KNAW Fungal Biodiversity Centre, Utrecht, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ilse","family":"Curfs-Breuker","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Infectious Diseases, Canisius Wilhelmina Hospital, Nijmegen, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Johan W.","family":"Mouton","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Infectious Diseases, Canisius Wilhelmina Hospital, Nijmegen, The Netherlands"},{"name":"Department of Medical Microbiology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andy I. M.","family":"Hoepelman","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine and Infectious Diseases, University Medical Centre Utrecht, Utrecht, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lodewijk","family":"Spanjaard","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Netherlands Reference Laboratory for Bacterial Meningitis, Academic Medical Center Amsterdam, Amsterdam, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul E.","family":"Verweij","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Greetje A.","family":"Kampinga","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, University Medical Center Groningen, Groningen, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ed J.","family":"Kuijper","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Center of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Teun","family":"Boekhout","sequence":"additional","affiliation":[{"name":"Department of Yeast and Basidiomycete Research, CBS-KNAW Fungal Biodiversity Centre, Utrecht, The Netherlands"},{"name":"Department of Internal Medicine and Infectious Diseases, University Medical Centre Utrecht, Utrecht, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Corn\u00e9 H. 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