{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T18:25:51Z","timestamp":1783189551065,"version":"3.54.6"},"reference-count":33,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2012,8,1]],"date-time":"2012-08-01T00:00:00Z","timestamp":1343779200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2012,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Helicobacter cinaedi<\/jats:named-content>\n            colonizes the colons of human and animals and can cause colitis, cellulitis, and sepsis in humans, with infections in immunocompromised patients being increasingly recognized. However, methods for analyzing the molecular epidemiology of\n            <jats:named-content content-type=\"genus-species\">H. cinaedi<\/jats:named-content>\n            are not yet established. A genotyping method involving multilocus sequence typing (MLST) was developed and used to analyze 50\n            <jats:named-content content-type=\"genus-species\">H. cinaedi<\/jats:named-content>\n            isolates from Japanese hospitals in addition to 6 reference strains. Pulsed-field gel electrophoresis (PFGE) results were also compared with the MLST results. Based on the genomic information from strain CCUG18818, 21 housekeeping genes were selected as candidates for MLST and were observed to have high homology (96.5 to 100%) between isolates. Following a comparison of the 21 housekeeping genes from 8\n            <jats:named-content content-type=\"genus-species\">H. cinaedi<\/jats:named-content>\n            isolates, 7 genes were chosen for MLST, revealing 14 sequence types (STs). The isolates from 3 hospitals belonged to the same STs, but the isolates from the other 4 hospitals belonged to different STs. Isolates belonging to ST6 were analyzed by PFGE and showed similar, but not identical, patterns between isolates. Isolates belonging to ST9, ST10, and ST11, which belonged to the same clonal complex, had the same pattern. All isolates were found to contain mutations in GyrA and the 23S rRNA gene that confer ciprofloxacin and clarithromycin resistance, respectively, in\n            <jats:named-content content-type=\"genus-species\">H. cinaedi<\/jats:named-content>\n            . These results raise concerns about the increase in\n            <jats:named-content content-type=\"genus-species\">H. cinaedi<\/jats:named-content>\n            isolates resistant to clarithromycin and ciprofloxacin in Japan.\n          <\/jats:p>","DOI":"10.1128\/jcm.06810-11","type":"journal-article","created":{"date-parts":[[2012,5,17]],"date-time":"2012-05-17T17:07:32Z","timestamp":1337274452000},"page":"2553-2560","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":39,"title":["Molecular Epidemiologic Analysis and Antimicrobial Resistance of Helicobacter cinaedi Isolated from Seven Hospitals in Japan"],"prefix":"10.1128","volume":"50","author":[{"given":"Emiko","family":"Rimbara","sequence":"first","affiliation":[{"name":"Department of Bacteriology II, National Institute of Infectious Diseases, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigetarou","family":"Mori","sequence":"additional","affiliation":[{"name":"Department of Bacteriology II, National Institute of Infectious Diseases, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mari","family":"Matsui","sequence":"additional","affiliation":[{"name":"Department of Bacteriology II, National Institute of Infectious Diseases, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satowa","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Department of Bacteriology II, National Institute of Infectious Diseases, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun-ichi","family":"Wachino","sequence":"additional","affiliation":[{"name":"Department of Bacteriology II, National Institute of Infectious Diseases, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshiaki","family":"Kawamura","sequence":"additional","affiliation":[{"name":"Department of Microbiology, School of Pharmacy, Aichi Gakuin University, Aichi, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zeli","family":"Shen","sequence":"additional","affiliation":[{"name":"Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James G.","family":"Fox","sequence":"additional","affiliation":[{"name":"Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keigo","family":"Shibayama","sequence":"additional","affiliation":[{"name":"Department of Bacteriology II, National Institute of Infectious Diseases, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/20.3.564"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.12.4071-4080.1999"},{"key":"e_1_3_3_4_2","volume-title":"Approved standard","author":"Clinical and Laboratory Standards Institute","year":"2012","unstructured":"Clinical and Laboratory Standards Institute. 2012. 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