{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T06:36:06Z","timestamp":1759991766743},"reference-count":0,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[1982,7,1]],"date-time":"1982-07-01T00:00:00Z","timestamp":394329600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[1982,7]]},"abstract":"<jats:p>\n            A direct, rapid, and simple method for the detection of streptococcal antigens of Lancefield groups A, B, C, D, and G from blood cultures was developed by using a coagglutination test. Fifty-five clinical specimens and 117 simulated blood cultures containing gram-positive cocci were tested. Out of 6,261 clinical blood cultures screened, 55 cultures from 53 patients were positive, with organisms resembling streptococci, by Gram stain. Of these cultures, 78% (43 of 55) were pure cultures of streptococci, and 22% (12 of 55) were mixed with at least one other organism. Of the 43 pure cultures only, correct reactions were obtained (grouping correctly or giving no cross-reactions, or both) with 86% (37 of 43) of the isolates, 12% (5 of 43) exhibited cross-reactions, and 2% (1 of 43) gave false-negative reactions. All of the cross-reacting isolates were\n            <jats:italic>Streptococcus pneumoniae<\/jats:italic>\n            , which reacted with the group C reagent, and the false-negative reaction occurred with a\n            <jats:italic>Streptococcus bovis<\/jats:italic>\n            isolate. However, by using a direct modified bile solubility test, the correct identification of the\n            <jats:italic>S. pneumoniae<\/jats:italic>\n            isolates was obtained. Therefore, by using the modified bile solubility test in conjunction with the direct grouping method, 98% (42 of 43) of the isolates in pure culture could be identified accurately and rapidly after the detection of a positive Gram stain. Correct grouping reactions were obtained with 83% (10 of 12) of the mixed blood cultures, and false-negative results occurred with 17% (2 of 12) of them. Both cultures contained an enterococcus and a gram-negative rod. Of the 117 simulated blood cultures, there was only one incorrect grouping reaction; this occurred with an\n            <jats:italic>S. bovis<\/jats:italic>\n            isolate that cross-reacted with the group C reagent. The direct grouping reaction was positive when blood cultures contained a minimum of 1 \u00d7 10\n            <jats:sup>8<\/jats:sup>\n            to 8 \u00d7 10\n            <jats:sup>8<\/jats:sup>\n            colony-forming units per ml. In general, this procedure provided information on the identification of the organism 24 h earlier than by conventional identification methods.\n          <\/jats:p>","DOI":"10.1128\/jcm.16.1.86-91.1982","type":"journal-article","created":{"date-parts":[[2020,1,5]],"date-time":"2020-01-05T02:34:28Z","timestamp":1578191668000},"page":"86-91","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":14,"title":["Direct Testing of Blood Cultures for Detection of Streptococcal Antigens"],"prefix":"10.1128","volume":"16","author":[{"given":"Maryellen A.","family":"Wetkowski","sequence":"first","affiliation":[{"name":"Division of Medical Microbiology, Department of Pathology, University of California Irvine Medical Center, Orange, California 92668"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ellena M.","family":"Peterson","sequence":"additional","affiliation":[{"name":"Division of Medical Microbiology, Department of Pathology, University of California Irvine Medical Center, Orange, California 92668"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis M.","family":"De La Maza","sequence":"additional","affiliation":[{"name":"Division of Medical Microbiology, Department of Pathology, University of California Irvine Medical Center, Orange, California 92668"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jcm.16.1.86-91.1982","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jcm.16.1.86-91.1982","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,4]],"date-time":"2022-03-04T21:57:39Z","timestamp":1646431059000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/jcm.16.1.86-91.1982"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1982,7]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1982,7]]}},"alternative-id":["10.1128\/jcm.16.1.86-91.1982"],"URL":"https:\/\/doi.org\/10.1128\/jcm.16.1.86-91.1982","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1982,7]]},"assertion":[{"value":"1982-07-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}