{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T05:45:50Z","timestamp":1774676750284,"version":"3.50.1"},"reference-count":0,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[1988,4,1]],"date-time":"1988-04-01T00:00:00Z","timestamp":575856000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[1988,4]]},"abstract":"<jats:p>Normal blood donors were examined for human immunodeficiency virus (HIV)-reactive antibodies with both virus- and Escherichia coli-expressed env- and gag-coded antigens. The frequency of samples from normal (low-risk) donors that were repeatedly reactive with an HIV enzyme-linked immunosorbent assay blood screening test (Du Pont Co.) was 0.6%. Two classes of HIV serological reactivity were identified: a minor env-reactive class (0.03 to 0.06% of donors) and the predominant env-nonreactive gag-reactive class (gag reactive only [GRO]) (0.4 to 0.5% of donors). Assignment of env reactivity was made by a synthetic (recombinant) env enzyme-linked immunosorbent assay and virus immunoblot. Most GRO sera reacted with p15\/p17 bands on HIV immunoblot. Antibody specificity in GRO sera was confirmed by competition-binding studies with viral gag and E. coli-expressed p55gag. This study provides independent verification that gag-specific antibodies are present in many env-nonreactive sera. More serological and virological studies of individuals with this antibody pattern should be pursued to determine the origin of these gag-reactive antibodies.<\/jats:p>","DOI":"10.1128\/jcm.26.4.641-647.1988","type":"journal-article","created":{"date-parts":[[2020,1,5]],"date-time":"2020-01-05T03:13:13Z","timestamp":1578193993000},"page":"641-647","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":17,"title":["Antibodies reactive with human immunodeficiency virus gag-coded antigens (gag reactive only) are a major cause of enzyme-linked immunosorbent assay reactivity in a blood donor population"],"prefix":"10.1128","volume":"26","author":[{"given":"D E","family":"Tribe","sequence":"first","affiliation":[{"name":"Medical Products Department, E. I. du Pont de Nemours and Company, Wilmington, Delaware 19898."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D L","family":"Reed","sequence":"additional","affiliation":[{"name":"Medical Products Department, E. I. du Pont de Nemours and Company, Wilmington, Delaware 19898."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P","family":"Lindell","sequence":"additional","affiliation":[{"name":"Medical Products Department, E. I. du Pont de Nemours and Company, Wilmington, Delaware 19898."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W R","family":"Kenealy","sequence":"additional","affiliation":[{"name":"Medical Products Department, E. I. du Pont de Nemours and Company, Wilmington, Delaware 19898."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B Q","family":"Ferguson","sequence":"additional","affiliation":[{"name":"Medical Products Department, E. I. du Pont de Nemours and Company, Wilmington, Delaware 19898."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R","family":"Cybulski","sequence":"additional","affiliation":[{"name":"Medical Products Department, E. I. du Pont de Nemours and Company, Wilmington, Delaware 19898."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D","family":"Winslow","sequence":"additional","affiliation":[{"name":"Medical Products Department, E. I. du Pont de Nemours and Company, Wilmington, Delaware 19898."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D M","family":"Waselefsky","sequence":"additional","affiliation":[{"name":"Medical Products Department, E. I. du Pont de Nemours and Company, Wilmington, Delaware 19898."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr","given":"S R","family":"Petteway","sequence":"additional","affiliation":[{"name":"Medical Products Department, E. I. du Pont de Nemours and Company, Wilmington, Delaware 19898."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jcm.26.4.641-647.1988","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jcm.26.4.641-647.1988","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,4]],"date-time":"2022-03-04T22:39:20Z","timestamp":1646433560000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/jcm.26.4.641-647.1988"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1988,4]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1988,4]]}},"alternative-id":["10.1128\/jcm.26.4.641-647.1988"],"URL":"https:\/\/doi.org\/10.1128\/jcm.26.4.641-647.1988","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1988,4]]},"assertion":[{"value":"1988-04-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}