{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T10:41:53Z","timestamp":1761129713992},"reference-count":0,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[1989,6,1]],"date-time":"1989-06-01T00:00:00Z","timestamp":612662400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[1989,6]]},"abstract":"<jats:p>A total of 114 serum specimens from 76 blood donors, 21 patients with acquired immune deficiency syndrome or acquired immune deficiency syndrome-related complex, 7 multiply transfused patients, 3 hemophiliacs, and 7 others were tested for anti-human immunodeficiency virus type 1 (HIV-1) antibody by enzyme immunoassay (EIA) and Western blot (WB) and then blindly tested by immunofluorescence (IF), independently, in two separate laboratories. The IF technique used acetone-fixed HIV-1-infected E cells and uninfected HUT-78 cells mixed at a 1:3 ratio in one spot on a glass slide and uninfected HUT-78 cells (to assess nonspecific fluorescence) alone in a second spot. Of 114 serum specimens, 85 were repeat EIA positive, and 21 of these were WB positive. A total of 129 of 134 of the IF results (included were 20 duplicates) were identical between laboratories, for a Kappa agreement statistic of 0.93. All five IF results discordant between laboratories were EIA repeat positive and WB negative. Included in the study were eight WB-indeterminate sera, of which five blood donor serum specimens and one hemophiliac serum specimen were IF negative and two acquired immune deficiency syndrome serum specimens were IF positive. As a confirmatory test for HIV-1 antibodies, IF provided a faster alternative or supplementary test for confirming EIA results.<\/jats:p>","DOI":"10.1128\/jcm.27.6.1234-1237.1989","type":"journal-article","created":{"date-parts":[[2020,1,5]],"date-time":"2020-01-05T03:21:28Z","timestamp":1578194488000},"page":"1234-1237","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":8,"title":["Agreement study between two laboratories of immunofluorescence as a confirmatory test for human immunodeficiency virus type 1 antibody screening"],"prefix":"10.1128","volume":"27","author":[{"given":"J","family":"Mahony","sequence":"first","affiliation":[{"name":"McMaster University Regional Virology Laboratory at St. Joseph's Hospital, Hamilton, Ontario, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K","family":"Rosenthal","sequence":"additional","affiliation":[{"name":"McMaster University Regional Virology Laboratory at St. Joseph's Hospital, Hamilton, Ontario, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Chernesky","sequence":"additional","affiliation":[{"name":"McMaster University Regional Virology Laboratory at St. Joseph's Hospital, Hamilton, Ontario, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Castriciano","sequence":"additional","affiliation":[{"name":"McMaster University Regional Virology Laboratory at St. Joseph's Hospital, Hamilton, Ontario, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E","family":"Scheid","sequence":"additional","affiliation":[{"name":"McMaster University Regional Virology Laboratory at St. Joseph's Hospital, Hamilton, Ontario, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Blajchman","sequence":"additional","affiliation":[{"name":"McMaster University Regional Virology Laboratory at St. Joseph's Hospital, Hamilton, Ontario, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D","family":"Harnish","sequence":"additional","affiliation":[{"name":"McMaster University Regional Virology Laboratory at St. Joseph's Hospital, Hamilton, Ontario, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jcm.27.6.1234-1237.1989","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jcm.27.6.1234-1237.1989","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,4]],"date-time":"2022-03-04T22:46:25Z","timestamp":1646433985000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/jcm.27.6.1234-1237.1989"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,6]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1989,6]]}},"alternative-id":["10.1128\/jcm.27.6.1234-1237.1989"],"URL":"https:\/\/doi.org\/10.1128\/jcm.27.6.1234-1237.1989","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"type":"print","value":"0095-1137"},{"type":"electronic","value":"1098-660X"}],"subject":[],"published":{"date-parts":[[1989,6]]},"assertion":[{"value":"1989-06-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}