{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,2]],"date-time":"2022-04-02T08:56:31Z","timestamp":1648889791731},"reference-count":0,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[1990,9,1]],"date-time":"1990-09-01T00:00:00Z","timestamp":652147200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[1990,9]]},"abstract":"<jats:p>We compared an alkaline phosphatase-conjugated oligonucleotide DNA probe with the Sereny test to determine the sensitivity and specificity of the probe in detecting virulent Shigella strains. The probe hybridized with all 52 Sereny-test-positive strains (sensitivity, 100%) and 4 of 21 Sereny-test-negative strains (specificity, 81%). The probe did not hybridize with any of the Sereny-test-negative S. dysenteriae type 1 strains. This nonradioactive, synthetic probe provides a simple, rapid way to test a large number of strains simultaneously in a field setting, which will contribute to an improved understanding of the epidemiologic patterns of shigellosis in developing countries.<\/jats:p>","DOI":"10.1128\/jcm.28.9.2122-2124.1990","type":"journal-article","created":{"date-parts":[[2020,1,5]],"date-time":"2020-01-05T03:30:58Z","timestamp":1578195058000},"page":"2122-2124","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":4,"title":["Comparison of alkaline phosphatase-conjugated oligonucleotide DNA probe with the Sereny test for identification of Shigella strains"],"prefix":"10.1128","volume":"28","author":[{"given":"C S","family":"Panda","sequence":"first","affiliation":[{"name":"Department of Microbiology, Lady Hardinge Medical College, New Delhi, India."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L W","family":"Riley","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Lady Hardinge Medical College, New Delhi, India."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S N","family":"Kumari","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Lady Hardinge Medical College, New Delhi, India."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K K","family":"Khanna","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Lady Hardinge Medical College, New Delhi, India."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K","family":"Prakash","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Lady Hardinge Medical College, New Delhi, India."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jcm.28.9.2122-2124.1990","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jcm.28.9.2122-2124.1990","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,4]],"date-time":"2022-03-04T22:56:18Z","timestamp":1646434578000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/jcm.28.9.2122-2124.1990"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,9]]},"references-count":0,"journal-issue":{"issue":"9","published-print":{"date-parts":[[1990,9]]}},"alternative-id":["10.1128\/jcm.28.9.2122-2124.1990"],"URL":"https:\/\/doi.org\/10.1128\/jcm.28.9.2122-2124.1990","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,9]]},"assertion":[{"value":"1990-09-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}