{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:28:59Z","timestamp":1791332939744,"version":"4.3.1"},"reference-count":0,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[1992,4,1]],"date-time":"1992-04-01T00:00:00Z","timestamp":702086400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[1992,4]]},"abstract":"<jats:p>Previous studies of in vitro infection by human T-cell lymphoma\/leukemia virus type I (HTLV-I) have required cocultivation of target cells with HTLV-I cell lines or vesicular stomatitis virus pseudotypes containing HTLV-I envelope proteins. We report here the development of a cell-free infection assay for HTLV-I. Target cells were incubated with purified, DNase-treated HTLV-I virions for 4 h at 37 degrees C. Target cell DNA was then analyzed for the presence of newly synthesized HTLV-I proviral DNA by the highly sensitive polymerase chain reaction. Using this assay system, we have been able to consistently detect in vitro infection of a variety of cellular targets by different HTLV-I isolates. Optimal infection required the presence of 10 micrograms of DEAE-dextran per ml. The assay was dose dependent with respect to virus input. In general, the amount of proviral DNA detected correlated with the level of HTLV-I receptors present on the surface of the target cells, as measured by fluorochrome-labelled HTLV-I binding. Finally, the specificity of the assay was confirmed by demonstrating that the cell line, L1q, a somatic cell hybrid containing human chromosome 17q, to which the gene for the HTLV-I receptor has been mapped, was susceptible to infection by HTLV-I, while the parental mouse cell line from which it was derived, LMTK-, which lacks human chromosome 17q, was not.<\/jats:p>","DOI":"10.1128\/jcm.30.4.905-910.1992","type":"journal-article","created":{"date-parts":[[2020,1,5]],"date-time":"2020-01-05T23:48:33Z","timestamp":1578268113000},"page":"905-910","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":111,"title":["Infection of peripheral blood mononuclear cells and cell lines by cell-free human T-cell lymphoma\/leukemia virus type I"],"prefix":"10.1128","volume":"30","author":[{"given":"N","family":"Fan","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, SUNY Health Science Center, Syracuse."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J","family":"Gavalchin","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, SUNY Health Science Center, Syracuse."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B","family":"Paul","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, SUNY Health Science Center, Syracuse."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K H","family":"Wells","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, SUNY Health Science Center, Syracuse."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M J","family":"Lane","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, SUNY Health Science Center, Syracuse."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B J","family":"Poiesz","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, SUNY Health Science Center, Syracuse."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jcm.30.4.905-910.1992","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jcm.30.4.905-910.1992","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,4]],"date-time":"2022-03-04T23:06:38Z","timestamp":1646435198000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/jcm.30.4.905-910.1992"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,4]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1992,4]]}},"alternative-id":["10.1128\/jcm.30.4.905-910.1992"],"URL":"https:\/\/doi.org\/10.1128\/jcm.30.4.905-910.1992","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,4]]},"assertion":[{"value":"1992-04-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}