{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T09:13:53Z","timestamp":1760346833123},"reference-count":0,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[1995,8,1]],"date-time":"1995-08-01T00:00:00Z","timestamp":807235200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[1995,8]]},"abstract":"<jats:p>Shigella serogrouping antisera from six companies (Becton Dickinson, Denka, Difco, Murex, Roach, and Sanofi-Pasteur) intended for the slide agglutination test and those of the Wellcolex Colour Shigella latex agglutination test were evaluated to identify quality products for Shigella identification. Forty-six reference Shigella strains (one for each serotype and species), 50 clinical strains (21 S. flexneri, 21 S. sonnei, 4 S. dysenteriae, 4 S. boydii) representing the most prevalent species and serotypes encountered in Quebec, and 9 non-Shigella strains were tested according to the manufacturers' instructions. A 3+ reaction (&gt; or = 75% agglutination) was considered positive for the slide agglutination tests. Sensitivity varied from 47% (Roach) to 94% (Difco). For the 105 strains tested, accuracy ranged from 53% (Roach) to 91% (Wellcolex). Specificity varied from 97 to 100% for group A antisera, from 96 to 100% for group B antisera, from 88 to 100% for group C antisera, and from 95 to 99% for group D antisera. The costs of reagents required to test one strain varied from $3.50 to $13.20 (in Canadian dollars). In conclusion, Roach reagents proved to be unsatisfactory for Shigella serogrouping. Among those from the remaining companies, the Denka, Difco, and Wellcolex reagents met a performance standard of 90% accuracy.<\/jats:p>","DOI":"10.1128\/jcm.33.8.1997-2001.1995","type":"journal-article","created":{"date-parts":[[2020,1,6]],"date-time":"2020-01-06T20:58:45Z","timestamp":1578344325000},"page":"1997-2001","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":21,"title":["Evaluation of commercial antisera for Shigella serogrouping"],"prefix":"10.1128","volume":"33","author":[{"given":"J","family":"Lefebvre","sequence":"first","affiliation":[{"name":"Bacterial Identification Section, Laboratoire de Sant\u00e9 Publique du Qu\u00e9bec, Ste-Anne-de-Bellevue, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F","family":"Gosselin","sequence":"additional","affiliation":[{"name":"Bacterial Identification Section, Laboratoire de Sant\u00e9 Publique du Qu\u00e9bec, Ste-Anne-de-Bellevue, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"Isma\u00efl","sequence":"additional","affiliation":[{"name":"Bacterial Identification Section, Laboratoire de Sant\u00e9 Publique du Qu\u00e9bec, Ste-Anne-de-Bellevue, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Lorange","sequence":"additional","affiliation":[{"name":"Bacterial Identification Section, Laboratoire de Sant\u00e9 Publique du Qu\u00e9bec, Ste-Anne-de-Bellevue, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H","family":"Lior","sequence":"additional","affiliation":[{"name":"Bacterial Identification Section, Laboratoire de Sant\u00e9 Publique du Qu\u00e9bec, Ste-Anne-de-Bellevue, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D","family":"Woodward","sequence":"additional","affiliation":[{"name":"Bacterial Identification Section, Laboratoire de Sant\u00e9 Publique du Qu\u00e9bec, Ste-Anne-de-Bellevue, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jcm.33.8.1997-2001.1995","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jcm.33.8.1997-2001.1995","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T07:39:02Z","timestamp":1646465942000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/jcm.33.8.1997-2001.1995"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,8]]},"references-count":0,"journal-issue":{"issue":"8","published-print":{"date-parts":[[1995,8]]}},"alternative-id":["10.1128\/jcm.33.8.1997-2001.1995"],"URL":"https:\/\/doi.org\/10.1128\/jcm.33.8.1997-2001.1995","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,8]]},"assertion":[{"value":"1995-08-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}