{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T21:15:08Z","timestamp":1780607708089,"version":"3.54.1"},"reference-count":0,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[1996,2,1]],"date-time":"1996-02-01T00:00:00Z","timestamp":823132800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[1996,2]]},"abstract":"<jats:p>Synthetic peptides, derived from the amino acid sequence of a Leishmania donovani clone, were used to develop an enzyme-linked immunosorbent assay (ELISA) for detecting antibodies against L. donovani. For this purpose, five peptides were conjugated to a protein carrier, human serum albumin (HSA), by using a heterobifunctional reagent, epsilon-maleimidocaproic acid N-hydroxysuccinimide ester, to obtain a well-defined product. The sensitivity and the specificity of the peptide-specific ELISA were determined with a panel of 106 serum samples from individuals living in areas where visceral leishmaniasis is endemic; sera from post-kala azar dermal leishmaniasis-infected patients and from individuals suffering from other infectious diseases were also included. ELISAs were performed with either a single peptide-HSA conjugate or a mixture of two peptide-HSA conjugates. Ninety-seven percent of the serum samples from patients with visceral leishmaniasis had detectable antibodies to one or more of the single synthetic peptides. ELISA with a single peptide-HSA conjugate proved to be less sensitive (less than 71%) but more specific (up to 93%) than ELISA with crude promastigote antigens (80% sensitivity and 79% specificity); when a combination of two different peptide-HSA conjugates was used, the test increased both in sensitivity and in specificity. Chemically defined peptide-protein conjugates improve the reproducibility and reliability of ELISA for the serodiagnosis of L. donovani infection.<\/jats:p>","DOI":"10.1128\/jcm.34.2.241-248.1996","type":"journal-article","created":{"date-parts":[[2020,1,6]],"date-time":"2020-01-06T21:04:22Z","timestamp":1578344662000},"page":"241-248","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":26,"title":["Synthetic peptide-based enzyme-linked immunosorbent assay for serodiagnosis of visceral leishmaniasis"],"prefix":"10.1128","volume":"34","author":[{"given":"C","family":"Fargeas","sequence":"first","affiliation":[{"name":"Glycobiologie, Centre National de la Recherche Scientifique, Orl\u00e9ans, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Hommel","sequence":"additional","affiliation":[{"name":"Glycobiologie, Centre National de la Recherche Scientifique, Orl\u00e9ans, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R","family":"Maingon","sequence":"additional","affiliation":[{"name":"Glycobiologie, Centre National de la Recherche Scientifique, Orl\u00e9ans, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C","family":"Dourado","sequence":"additional","affiliation":[{"name":"Glycobiologie, Centre National de la Recherche Scientifique, Orl\u00e9ans, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Monsigny","sequence":"additional","affiliation":[{"name":"Glycobiologie, Centre National de la Recherche Scientifique, Orl\u00e9ans, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R","family":"Mayer","sequence":"additional","affiliation":[{"name":"Glycobiologie, Centre National de la Recherche Scientifique, Orl\u00e9ans, France."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jcm.34.2.241-248.1996","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jcm.34.2.241-248.1996","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T07:55:30Z","timestamp":1646466930000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/jcm.34.2.241-248.1996"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,2]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1996,2]]}},"alternative-id":["10.1128\/jcm.34.2.241-248.1996"],"URL":"https:\/\/doi.org\/10.1128\/jcm.34.2.241-248.1996","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,2]]},"assertion":[{"value":"1996-02-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}