{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T05:31:09Z","timestamp":1791351069943,"version":"4.3.1"},"reference-count":0,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[1997,3,1]],"date-time":"1997-03-01T00:00:00Z","timestamp":857174400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[1997,3]]},"abstract":"<jats:p>In this study we have devised a simple and robust PCR strategy to detect a wide range of viruses, bacteria, and parasites, all of which are capable of causing aseptic meningitis and encephalitis. The techniques developed have been used in a routine diagnostic virology laboratory to test prospectively 2,233 cerebrospinal fluid specimens. A virus was detected in 147 specimens of cerebrospinal fluid from 143 patients. Four sets of primers were sufficient to detect the virus in 135 (94%) of the PCR-positive patients. We conclude that with appropriate primers, PCR can be systematically and economically applied to test for a range of organisms in a routine diagnostic laboratory. In our opinion, PCR will soon become the \"gold standard\" test for viral infections of the central nervous system.<\/jats:p>","DOI":"10.1128\/jcm.35.3.691-696.1997","type":"journal-article","created":{"date-parts":[[2020,1,6]],"date-time":"2020-01-06T21:14:20Z","timestamp":1578345260000},"page":"691-696","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":105,"title":["Aseptic meningitis and encephalitis: the role of PCR in the diagnostic laboratory"],"prefix":"10.1128","volume":"35","author":[{"given":"S J","family":"Read","sequence":"first","affiliation":[{"name":"Oxford Public Health Laboratory, John Radcliffe Hospital, United Kingdom."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K J","family":"Jeffery","sequence":"additional","affiliation":[{"name":"Oxford Public Health Laboratory, John Radcliffe Hospital, United Kingdom."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C R","family":"Bangham","sequence":"additional","affiliation":[{"name":"Oxford Public Health Laboratory, John Radcliffe Hospital, United Kingdom."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jcm.35.3.691-696.1997","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jcm.35.3.691-696.1997","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T08:16:06Z","timestamp":1646468166000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/jcm.35.3.691-696.1997"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,3]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1997,3]]}},"alternative-id":["10.1128\/jcm.35.3.691-696.1997"],"URL":"https:\/\/doi.org\/10.1128\/jcm.35.3.691-696.1997","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,3]]},"assertion":[{"value":"1997-03-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}