{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T10:14:37Z","timestamp":1783419277678,"version":"3.54.6"},"reference-count":0,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[1997,3,1]],"date-time":"1997-03-01T00:00:00Z","timestamp":857174400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[1997,3]]},"abstract":"<jats:p>Cyclospora, a coccidian protist, is increasingly being identified as an important, newly emerging parasite that causes diarrhea, flatulence, fatigue, and abdominal pain leading to weight loss in immunocompetent persons with or without a recent travel history as well as in patients with AIDS. Modified Kinyoun's acid-fast stain is the most commonly used stain to identify the oocyst of this parasite in fecal smears. Oocysts of Cyclospora stain variably by the modified acid-fast procedure, resulting in the possible misidentification of this parasite. We examined fecal smears stained by six different procedures that included Giemsa, trichrome, chromotrope, Gram-chromotrope, acid-fast, and safranin stains. We report on safranin-based stain that uniformly stains oocysts of Cyclospora a brilliant reddish orange, provided that the fecal smears are heated in a microwave oven prior to staining. This staining procedure, besides being superior to acid-fast staining, is fast, reliable, and easy to perform in most clinical laboratories.<\/jats:p>","DOI":"10.1128\/jcm.35.3.730-733.1997","type":"journal-article","created":{"date-parts":[[2020,1,6]],"date-time":"2020-01-06T21:14:24Z","timestamp":1578345264000},"page":"730-733","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":87,"title":["Uniform staining of Cyclospora oocysts in fecal smears by a modified safranin technique with microwave heating"],"prefix":"10.1128","volume":"35","author":[{"given":"G S","family":"Visvesvara","sequence":"first","affiliation":[{"name":"Division of Parasitic Diseases, National Cancer for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30341-3724, USA. gsvl@ciddpd2.em.cdc.gov"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H","family":"Moura","sequence":"additional","affiliation":[{"name":"Division of Parasitic Diseases, National Cancer for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30341-3724, USA. gsvl@ciddpd2.em.cdc.gov"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E","family":"Kovacs-Nace","sequence":"additional","affiliation":[{"name":"Division of Parasitic Diseases, National Cancer for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30341-3724, USA. gsvl@ciddpd2.em.cdc.gov"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Wallace","sequence":"additional","affiliation":[{"name":"Division of Parasitic Diseases, National Cancer for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30341-3724, USA. gsvl@ciddpd2.em.cdc.gov"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M L","family":"Eberhard","sequence":"additional","affiliation":[{"name":"Division of Parasitic Diseases, National Cancer for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30341-3724, USA. gsvl@ciddpd2.em.cdc.gov"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jcm.35.3.730-733.1997","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jcm.35.3.730-733.1997","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T08:16:28Z","timestamp":1646468188000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/jcm.35.3.730-733.1997"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,3]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1997,3]]}},"alternative-id":["10.1128\/jcm.35.3.730-733.1997"],"URL":"https:\/\/doi.org\/10.1128\/jcm.35.3.730-733.1997","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,3]]},"assertion":[{"value":"1997-03-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}