{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T23:05:50Z","timestamp":1778540750611,"version":"3.51.4"},"reference-count":35,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[1998,1,1]],"date-time":"1998-01-01T00:00:00Z","timestamp":883612800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[1998,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            In this work, we describe the assembly of a synthetic gene coding for several antigenic determinants found in different\n            <jats:italic>Leishmania infantum<\/jats:italic>\n            antigens. Selected epitopes were derived from the ribosomal proteins LiP2a, LiP2b, and LiP0 and from the histone H2A. The resulting gene was overexpressed in\n            <jats:italic>Escherichia coli<\/jats:italic>\n            either as a fusion protein (with the vector pMAL-c2) or alone (with the vector pQE). In both cases, high-level bacterial production of the recombinant protein was achieved and the products were found to be stable. Enzyme-linked immunosorbent assay (ELISA) and Western blotting experiments confirmed that the corresponding epitopes are present in the engineered protein. Finally, a serological evaluation of this multiple-epitope protein by Falcon assay screening test-ELISA revealed a sensitivity of 79 to 93% and a specificity of 96 to 100% in diagnosis of canine visceral leishmaniasis, indicating that this protein represents a valuable tool for serodiagnosis.\n          <\/jats:p>","DOI":"10.1128\/jcm.36.1.58-63.1998","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T17:21:30Z","timestamp":1577812890000},"page":"58-63","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":62,"title":["Multicomponent Chimeric Antigen for Serodiagnosis of Canine Visceral Leishmaniasis"],"prefix":"10.1128","volume":"36","author":[{"given":"Manuel","family":"Soto","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Centro de Biolog\u0131\u0301a Molecular \u201cSevero Ochoa\u201d (CSIC-UAM), Universidad Auto\u0301noma de Madrid, 28049 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jose M.","family":"Requena","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Centro de Biolog\u0131\u0301a Molecular \u201cSevero Ochoa\u201d (CSIC-UAM), Universidad Auto\u0301noma de Madrid, 28049 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis","family":"Quijada","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Centro de Biolog\u0131\u0301a Molecular \u201cSevero Ochoa\u201d (CSIC-UAM), Universidad Auto\u0301noma de Madrid, 28049 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos","family":"Alonso","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Centro de Biolog\u0131\u0301a Molecular \u201cSevero Ochoa\u201d (CSIC-UAM), Universidad Auto\u0301noma de Madrid, 28049 Madrid, 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