{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,5]],"date-time":"2026-11-05T03:50:09Z","timestamp":1793850609271,"version":"4.6.1"},"reference-count":16,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[1998,1,1]],"date-time":"1998-01-01T00:00:00Z","timestamp":883612800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[1998,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            We have previously described the creation by Tn\n            <jats:italic>916<\/jats:italic>\n            mutagenesis of avirulent transposition mutants from a highly virulent strain of\n            <jats:italic>Erysipelothrix rhusiopathiae<\/jats:italic>\n            , the causative agent of swine erysipelas. In this study, we cloned a 2.2-kb DNA fragment which flanked the Tn\n            <jats:italic>916<\/jats:italic>\n            insertion in an avirulent mutant (strain 33H6) and evaluated the possibility that this region could be used for the specific detection of\n            <jats:italic>E. rhusiopathiae<\/jats:italic>\n            . According to the sequences of this region, oligonucleotide primers were designed to amplify a 937-bp fragment of the\n            <jats:italic>E. rhusiopathiae<\/jats:italic>\n            chromosome by PCR. The specificity of the PCR was investigated by analyzing 64 strains of\n            <jats:italic>Erysipelothrix<\/jats:italic>\n            species and 27 strains of other genera different from\n            <jats:italic>Erysipelothrix<\/jats:italic>\n            . A 937-bp DNA fragment could be amplified from all\n            <jats:italic>E. rhusiopathiae<\/jats:italic>\n            strains tested, and no amplification was observed by using DNAs from the other species tested. To make a rapid and definite diagnosis of swine erysipelas in slaughterhouses, we developed an enrichment broth cultivation-PCR combination assay, which used a commercially available DNA extraction kit, to identify\n            <jats:italic>E. rhusiopathiae<\/jats:italic>\n            in the specimens from swine with arthritis. After samples were enriched in selective broth culture, detection of\n            <jats:italic>E. rhusiopathiae<\/jats:italic>\n            was tested by either conventional methods or the PCR. Of 102 samples tested, 15 samples were positive by conventional methods and 12 of the 15 samples were positive by the PCR. The detection limit of the PCR was 10\n            <jats:sup>3<\/jats:sup>\n            CFU per reaction mixture for the PCR-positive samples. These results indicate that this PCR technique could be used as a first-line screening technique for the specific detection of\n            <jats:italic>E. rhusiopathiae<\/jats:italic>\n            in specimens.\n          <\/jats:p>","DOI":"10.1128\/jcm.36.1.86-89.1998","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T17:21:46Z","timestamp":1577812906000},"page":"86-89","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":47,"title":["Use of an Enrichment Broth Cultivation-PCR Combination Assay for Rapid Diagnosis of Swine Erysipelas"],"prefix":"10.1128","volume":"36","author":[{"given":"Yoshihiro","family":"Shimoji","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->National Institute of Animal Health, Ibaraki 305,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuyuki","family":"Mori","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->National Institute of Animal Health, Ibaraki 305,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koji","family":"Hyakutake","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Utsunomiya City Meat Inspection Office, Tochigi 321-01,2 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tsutomu","family":"Sekizaki","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->National Institute of Animal Health, Ibaraki 305,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuichi","family":"Yokomizo","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->National Institute of Animal Health, Ibaraki 305,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/7.6.1513"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.58.9.3116-3121.1990"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.159.1.214-221.1984"},{"key":"e_1_3_2_5_2","unstructured":"Graves\nL. M.\nSwaminathan\nB.\nUniversal bacterial DNA isolation procedure\nDiagnostic molecular microbiology: principles and applications.\nPersing\nD. H.\nSmith\nT. F.\nTenover\nF. C.\nWhite\nT. J.\n1993\n617\n621\nAmerican Society for Microbiology\nWashington D.C"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.32.6.1526-1531.1994"},{"key":"e_1_3_2_7_2","unstructured":"Sambrook\nJ.\nFritsch\nE. F.\nManiatis\nT.\nMolecular cloning: a laboratory manual\n2nd ed.\n1989\nCold Spring Harbor Laboratory Press\nCold Spring Harbor N.Y"},{"key":"e_1_3_2_8_2","first-page":"699","article-title":"DNA sequencing with chain-terminating inhibitors","volume":"86","author":"Sanger F.","year":"1977","unstructured":"Sanger F. Nicklen S. Coulson A. R. DNA sequencing with chain-terminating inhibitors.Proc. Natl. Acad. Sci. USA 86 1977 699 703","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.62.7.2806-2810.1994"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-37-2-166"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.25.3.536-539.1987"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-42-3-469"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.58.587"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1080\/03079459408418992"},{"key":"e_1_3_2_15_2","first-page":"1411","article-title":"Erysipelothrix arthritis in swine: bacteriologic and immunopathologic aspects","volume":"31","author":"Timoney J. F.","year":"1970","unstructured":"Timoney J. F. Jr. Berman D. T. Erysipelothrix arthritis in swine: bacteriologic and immunopathologic aspects.Am. J. Vet. Res. 31 1970 1411 1421","journal-title":"Am. J. Vet. Res."},{"key":"e_1_3_2_16_2","first-page":"944","article-title":"Swine erysipelas\u2014a review of prevalence and research","volume":"184","author":"Wood R. L.","year":"1984","unstructured":"Wood R. L. Swine erysipelas\u2014a review of prevalence and research.J. Am. Vet. Med. Assoc. 184 1984 944 949","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"e_1_3_2_17_2","unstructured":"Wood\nR. L.\nErysipelas\nDiseases of swine.\nLeman\nA. D.\n1992\n475\n486\nIowa State University Press\nAmes"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.36.1.86-89.1998","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.36.1.86-89.1998","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,4]],"date-time":"2022-03-04T23:33:32Z","timestamp":1646436812000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.36.1.86-89.1998"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,1]]},"references-count":16,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1998,1]]}},"alternative-id":["10.1128\/JCM.36.1.86-89.1998"],"URL":"https:\/\/doi.org\/10.1128\/jcm.36.1.86-89.1998","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,1]]},"assertion":[{"value":"1997-04-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1997-10-10","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"1998-01-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}