{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T15:13:39Z","timestamp":1793459619799,"version":"4.5.0"},"reference-count":26,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[1998,2,1]],"date-time":"1998-02-01T00:00:00Z","timestamp":886291200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[1998,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The gene (<jats:italic>omlA<\/jats:italic>) coding for an outer membrane protein of<jats:italic>Actinobacillus pleuropneumoniae<\/jats:italic>serotypes 1 and 5 has been described earlier and has formed the basis for development of a specific PCR assay. The corresponding regions of all 12<jats:italic>A. pleuropneumoniae<\/jats:italic>reference strains of biovar 1 were sequenced. Alignment of the sequences revealed conserved terminal and variable middle regions, which divided the reference strains into four distinct groups. Primers were selected from the conserved 5\u2032 and 3\u2032 termini of the gene. A 950-bp amplicon was obtained from each of 102 tested field isolates of<jats:italic>A. pleuropneumoniae<\/jats:italic>obtained from lungs. Their identity was verified by sequencing approximately 500 bp of the amplification product from 50 of the<jats:italic>A. pleuropneumoniae<\/jats:italic>isolates, which all showed the expected DNA sequence characteristic of the serotype. To test the specificity of the reaction, 23 other bacterial species related to<jats:italic>A. pleuropneumoniae<\/jats:italic>or isolated from pigs were assayed. They were all found negative in the PCR, as were tonsil cultures from 50 pigs of an<jats:italic>A. pleuropneumoniae<\/jats:italic>-negative herd. The sensitivity assessed by agarose gel analysis of the PCR product was 10<jats:sup>2<\/jats:sup>CFU\/PCR test tube. The specificity and sensitivity of this PCR compared to those of culture suggest the use of this PCR for routine identification of<jats:italic>A. pleuropneumoniae.<\/jats:italic><\/jats:p>","DOI":"10.1128\/jcm.36.2.443-448.1998","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T17:26:21Z","timestamp":1577813181000},"page":"443-448","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":55,"title":["Improved Diagnostic PCR Assay for<i>Actinobacillus pleuropneumoniae<\/i>Based on the Nucleotide Sequence of an Outer Membrane Lipoprotein"],"prefix":"10.1128","volume":"36","author":[{"given":"Trine","family":"Gram","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Danish Veterinary Laboratory, DK-1790 Copenhagen V, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Ahrens","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Danish Veterinary Laboratory, DK-1790 Copenhagen V, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.32.11.2749-2754.1994"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-41-1-121"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.63.7.2797-2800.1995"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1135(94)90064-7"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.174.6.2002-2013.1992"},{"key":"e_1_3_2_7_2","first-page":"303","article-title":"Comparative virulence of NAD-dependent and NAD-independent Actinobacillus pleuropneumoniae strains","volume":"39","author":"Dom P.","year":"1992","unstructured":"Dom P. 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