{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T18:01:52Z","timestamp":1763748112878},"reference-count":40,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[1998,2,1]],"date-time":"1998-02-01T00:00:00Z","timestamp":886291200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[1998,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The 16S rRNA gene of\n            <jats:italic>Haemobartonella felis<\/jats:italic>\n            was amplified by using universal eubacterial primers and was subsequently cloned and sequenced. Based on this sequence data, we designed a set of\n            <jats:italic>H. felis<\/jats:italic>\n            -specific primers. These primers selectively amplified a 1,316-bp DNA fragment of the 16S rRNA gene of\n            <jats:italic>H. felis<\/jats:italic>\n            from each of four experimentally infected cats at peak parasitemia. No PCR product was amplified from purified DNA of\n            <jats:italic>Eperythrozoon suis<\/jats:italic>\n            ,\n            <jats:italic>Mycoplasma genitalium<\/jats:italic>\n            , and\n            <jats:italic>Bartonella bacilliformis<\/jats:italic>\n            . Blood from the experimental cats prior to infection was negative for PCR products and was greatly diminished or absent 1 month after doxycycline treatment. The overall sequence identity of this fragment varied by less than 1.0% among experimentally infected cats. By taking into consideration the secondary structure of the 16S rRNA molecule, we were able to further verify the alignment of nucleotides and quality of our sequence data. In this PCR assay, the minimum detectable number of\n            <jats:italic>H. felis<\/jats:italic>\n            organisms was determined to be between 50 and 704. The potential usefulness of restriction enzymes\n            <jats:italic>Dde<\/jats:italic>\n            I and\n            <jats:italic>Mnl<\/jats:italic>\n            I for distinguishing\n            <jats:italic>H. felis<\/jats:italic>\n            from closely related bacteria was examined. This is the first report of the utility of PCR-facilitated diagnosis and discrimination of\n            <jats:italic>H. felis<\/jats:italic>\n            infection in cats.\n          <\/jats:p>","DOI":"10.1128\/jcm.36.2.462-466.1998","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T17:26:30Z","timestamp":1577813190000},"page":"462-466","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":81,"title":["Development and Evaluation of a PCR-Based Assay for Detection of\n            <i>Haemobartonella felis<\/i>\n            in Cats and Differentiation of\n            <i>H. felis<\/i>\n            from Related Bacteria by Restriction Fragment Length Polymorphism Analysis"],"prefix":"10.1128","volume":"36","author":[{"given":"Joanne B.","family":"Messick","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Department of Veterinary Pathobiology, The University of Illinois at Champaign-Urbana, Urbana, Illinois 61802"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linda M.","family":"Berent","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Veterinary Pathobiology, The University of Illinois at Champaign-Urbana, Urbana, Illinois 61802"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandra K.","family":"Cooper","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Veterinary Pathobiology, The University of Illinois at Champaign-Urbana, Urbana, Illinois 61802"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/16.5.1861"},{"key":"e_1_3_1_3_2","first-page":"261","article-title":"Differentiation of Bartonella species using restriction endonuclease analysis of PCR-amplified 16S rRNA genes","volume":"129","author":"Birtles R. J.","year":"1995","unstructured":"Birtles R. J. Differentiation of Bartonella species using restriction endonuclease analysis of PCR-amplified 16S rRNA genes.FEMS Microbiol. Lett. 129 1995 261 266","journal-title":"FEMS Microbiol. Lett."},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(91)90379-P"},{"key":"e_1_3_1_5_2","doi-asserted-by":"crossref","unstructured":"Brown D. R. G. S. McLaughlin and M. B. Brown. Taxonomy of feline mycoplasmas Mycoplasma felifaucium Mycoplasma feliminutum Mycoplasma felis Mycoplasma gateae Mycoplasma leocaptivus Mycoplasma leopharyngis and Mycoplasma simbae by 16S rRNA gene sequence comparisons. Int. J. Syst. Bacteriol. 45: 560\u2013564.","DOI":"10.1099\/00207713-45-3-560"},{"key":"e_1_3_1_6_2","doi-asserted-by":"crossref","unstructured":"Carney\nH. C.\nEngland\nJ. J.\nFeline hemobartonellosis\nThe veterinary clinics of North America: small animal practice (feline infectious diseases). W. B.\nHoskins\nJ. D.\nLoar\nA. S.\n1993\n79\n89\nSaunders Company\nPhiladelphia Pa","DOI":"10.1016\/S0195-5616(93)50005-0"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.1994.tb00084.x"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-45-3-192"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/12.1Part1.387"},{"key":"e_1_3_1_10_2","first-page":"45","article-title":"Infectious anemia in cats","volume":"122","author":"Flint J. C.","year":"1953","unstructured":"Flint J. C. Moss L. C. Infectious anemia in cats.J. Am. Vet. Med. Assoc. 122 1953 45 48","journal-title":"J. Am. Vet. Med. 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