{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T01:04:47Z","timestamp":1782781487211,"version":"3.54.5"},"reference-count":20,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[1998,3,1]],"date-time":"1998-03-01T00:00:00Z","timestamp":888710400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[1998,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A 20-ml blood sample was collected from adult patients with suspected bloodstream infections and distributed equally into the four volume-controlled bottles of a blood culture set consisting of aerobic and anaerobic BACTEC Plus\/F bottles and aerobic and anaerobic BacT\/Alert FAN bottles. All bottles were incubated in their respective instruments for a standard 5-day protocol or until the instruments signalled positivity. Samples in all bottles with negative results by these instruments were terminally subcultured. A total of 8,390 blood culture sets were obtained during the study period, of which 4,402 (52.5%) met the study criteria. Of these, 946 (21.5%) were positive either by instrument signal or by additional terminal subculture of all negative bottles and yielded growth of microorganisms. Five hundred eighty-nine (13.4%) blood culture sets were considered to have recovered 663 clinically significant organisms. When both the BACTEC and the BacT\/Alert systems were used, 465 positive sets were detected; BACTEC alone detected 52 positive sets and BacT\/Alert alone detected 72 (\n            <jats:italic>P<\/jats:italic>\n            = 0.09). No differences were found between the two systems in microbial recovery rate from blood cultures obtained from patients on antibiotic therapy. Significantly more members of the family\n            <jats:italic>Enterobacteriaceae<\/jats:italic>\n            (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.01) were detected from patients without antimicrobial therapy by BacT\/Alert than by BACTEC. The false-negative rates were 0.20% for BACTEC and 0.32% for BacT\/Alert. A significantly higher false-positive rate was found for BACTEC (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.0001). Both systems were comparable for the time to detection of microorganisms. However, gram-positive bacteria were detected faster by BACTEC and\n            <jats:italic>Enterobacteriaceae<\/jats:italic>\n            were detected faster on average by BacT\/Alert. We concluded that both systems are comparable in their abilities to recover aerobic and anaerobic organisms from blood cultures and a terminal subculture might not be necessary for either of the two systems. The increased positivity rate when using an anaerobic bottle in a two-bottle blood culture set is due to the additional blood volume rather than to the use of an anaerobic medium.\n          <\/jats:p>","DOI":"10.1128\/jcm.36.3.657-661.1998","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T17:27:33Z","timestamp":1577813253000},"page":"657-661","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":62,"title":["Controlled Clinical Laboratory Comparison of Two Supplemented Aerobic and Anaerobic Media Used in Automated Blood Culture Systems To Detect Bloodstream Infections"],"prefix":"10.1128","volume":"36","author":[{"given":"R.","family":"Ziegler","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Institut fu\u0308r Klinikhygiene, Medizinische Mikrobiologie und Klinische Infektiologie, Klinikum Nu\u0308rnberg, 90419 Nu\u0308rnberg,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Johnscher","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Institut fu\u0308r Klinikhygiene, Medizinische Mikrobiologie und Klinische Infektiologie, Klinikum Nu\u0308rnberg, 90419 Nu\u0308rnberg,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Martus","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Institut fu\u0308r Medizinische Statistik und Dokumentation, Universita\u0308t Erlangen-Nu\u0308rnberg, 91054 Erlangen,2 Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Lenhardt","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Institut fu\u0308r Klinikhygiene, Medizinische Mikrobiologie und Klinische Infektiologie, Klinikum Nu\u0308rnberg, 90419 Nu\u0308rnberg,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.-M.","family":"Just","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Institut fu\u0308r Klinikhygiene, Medizinische Mikrobiologie und Klinische Infektiologie, Klinikum Nu\u0308rnberg, 90419 Nu\u0308rnberg,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","unstructured":"Auckenthaler\nR.\nRohner\nP.\nVerschraegen\nG.\nBernard\nD.\nClaeys\nG.\nShafi\nM. S.\nStevens\nM.\nSchuster\nG.\nKontinuierlich messende Blutkultursysteme.European Congress of Clinical Microbiology and Infectious Diseases Seville Spain (M\u00e4rz 1993)\n1994"},{"key":"e_1_3_2_3_2","unstructured":"Fontanals\nD.\nStafeliu\nI.\nPons\nI.\nMariscal\nD.\nTorra\nM.\nEvaluation of the performance of BacT\/Alert and VITAL blood culture systems for diagnosis of bacteremia. Poster 16\/17 at the Seventh National Congress of the Spanish Society for Infectious Diseases and Clinical Microbiology Torremolinos Spain\n1996"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.30.10.2743-2745.1992"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.1.53-58.1997"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.31.8.2110-2113.1993"},{"key":"e_1_3_2_7_2","unstructured":"Pittet\nD.\nNosocomial bloodstream infections.Prevention and control of nosocomial infections\n3rd ed.\nWenzel\nR. 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F.\nIntravenous-related infections.Prevention and control of nosocomial infections\n3rd ed.\nWenzel\nR. 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