{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,29]],"date-time":"2024-07-29T13:14:53Z","timestamp":1722258893371},"reference-count":24,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[1998,3,1]],"date-time":"1998-03-01T00:00:00Z","timestamp":888710400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[1998,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Two flow cytometric assays are described herein. The single cytometric test (SCT) detects antibodies to either<jats:italic>Brucella abortus<\/jats:italic>or<jats:italic>Staphylococcus aureus<\/jats:italic>in the serum or milk of a cow or water buffalo. The double cytometric test (DCT) detects both anti-<jats:italic>B. abortus<\/jats:italic>and anti-<jats:italic>S. aureus<\/jats:italic>antibodies concurrently. In the SCT, the sample to be tested is incubated in succession with the antigen (either<jats:italic>B. abortus<\/jats:italic>or<jats:italic>S. aureus<\/jats:italic>) and the proper secondary antiserum (fluorescein isothiocyanate-labelled rabbit anti-cow immunoglobulin antiserum or rabbit anti-water buffalo immunoglobulin antiserum). In the DCT, the sample to be tested is incubated first with<jats:italic>B. abortus<\/jats:italic>and<jats:italic>S. aureus<\/jats:italic>antigens and then with the secondary antiserum. The<jats:italic>B. abortus<\/jats:italic>antigen used in the DCT is covalently bound to 3-\u03bcm-diameter latex particles. The difference in size between<jats:italic>B. abortus<\/jats:italic>and<jats:italic>S. aureus<\/jats:italic>permits the establishment of whether the antibodies are directed against one, the other, or both antigens. When compared to the complement fixation test, the SCT and DCT each show a specificity and a sensitivity of 100%. The SCT has been used previously to detect anti-<jats:italic>S. aureus<\/jats:italic>antibodies. Here its use is extended to the detection of anti-<jats:italic>B. abortus<\/jats:italic>antibodies. The DCT is described here for the first time. The DCT appears to be useful for large-scale brucellosis eradication programs. It offers the possibility of using one test to identify animals that are serologically positive for both<jats:italic>B. abortus<\/jats:italic>and<jats:italic>S. aureus.<\/jats:italic><\/jats:p>","DOI":"10.1128\/jcm.36.3.802-806.1998","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T17:28:25Z","timestamp":1577813305000},"page":"802-806","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["Simultaneous Identification of Antibodies to<i>Brucella abortus<\/i>and<i>Staphylococcus aureus<\/i>in Milk Samples by Flow Cytometry"],"prefix":"10.1128","volume":"36","author":[{"given":"Domenico","family":"Iannelli","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Chair of Immunology, School of Agriculture,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Letizia","family":"D\u2019Apice","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Istituto Zooprofilattico Sperimentale,2 Portici, 80055 Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Domenico","family":"Fenizia","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Istituto Zooprofilattico Sperimentale,2 Portici, 80055 Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luigi","family":"Serpe","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Istituto Zooprofilattico Sperimentale,2 Portici, 80055 Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlo","family":"Cottone","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Chair of Immunology, School of Agriculture,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maurizio","family":"Viscardi","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Chair of Immunology, School of Agriculture,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosanna","family":"Capparelli","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Chair of Immunology, School of Agriculture,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","unstructured":"Alton G. 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