{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T06:02:23Z","timestamp":1778047343699,"version":"3.51.4"},"reference-count":21,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[1998,4,1]],"date-time":"1998-04-01T00:00:00Z","timestamp":891388800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[1998,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Disseminated infection with the dimorphic pathogenic fungus\n            <jats:italic>Penicillium marneffei<\/jats:italic>\n            is increasingly seen among patients with AIDS in southeast Asian countries. Previous studies have demonstrated the presence of humoral immune responses to this fungus in patient sera; we have confirmed this work using sera from\n            <jats:italic>P. marneffei<\/jats:italic>\n            -infected patients (\n            <jats:italic>n<\/jats:italic>\n            = 21) to develop Western blots of\n            <jats:italic>P. marneffei<\/jats:italic>\n            cytoplasmic yeast antigen (CYA).\n            <jats:italic>P. marneffei<\/jats:italic>\n            CYA was then partially purified by liquid isoelectric focusing, and fractions were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting. Immunoenzyme development of the Western blots with pooled sera from patients with\n            <jats:italic>P. marneffei<\/jats:italic>\n            infection and with pooled sera from patients with aspergillosis (\n            <jats:italic>n<\/jats:italic>\n            = 20), candidiasis (\n            <jats:italic>n<\/jats:italic>\n            = 10), cryptococcosis (\n            <jats:italic>n<\/jats:italic>\n            = 9), and histoplasmosis (\n            <jats:italic>n<\/jats:italic>\n            = 11) revealed three antigens with relative molecular masses of 61, 54, and 50 kDa. These antigens were specifically recognized by the pooled sera from the\n            <jats:italic>P. marneffei<\/jats:italic>\n            -infected patients. The 61- and 54-kDa antigens were subsequently purified to homogeneity by preparative gel electrophoresis, and the 50-kDa antigen was partially purified by the same technique. N-terminal amino acid sequencing revealed that the 61-kDa antigen had a strong homology (87% identity) with the antioxidant enzyme catalase. The three antigens were then subjected to SDS-PAGE and Western blotting and to immunoenzyme development with individual patient sera; sera from 86% of\n            <jats:italic>P. marneffei<\/jats:italic>\n            -infected patients recognized the 61-kDa antigen, sera from 71% recognized the 54-kDa antigen, and sera from 48% recognized the 50-kDa antigen. These specifically recognized antigens are the first to be purified from\n            <jats:italic>P. marneffei<\/jats:italic>\n            and can be used either singly or in combination to detect antibody responses in a large percentage of individuals infected with\n            <jats:italic>P. marneffei.<\/jats:italic>\n          <\/jats:p>","DOI":"10.1128\/jcm.36.4.949-954.1998","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T17:29:19Z","timestamp":1577813359000},"page":"949-954","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":35,"title":["Identification and Purification of Specific\n            <i>Penicillium marneffei<\/i>\n            Antigens and Their Recognition by Human Immune Sera"],"prefix":"10.1128","volume":"36","author":[{"given":"L.","family":"Jeavons","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Dermatology Laboratory, St. John\u2019s Institute of Dermatology, Thomas Guy House, Guy\u2019s Hospital, London SE1-9RT,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. J.","family":"Hamilton","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Dermatology Laboratory, St. John\u2019s Institute of Dermatology, Thomas Guy House, Guy\u2019s Hospital, London SE1-9RT,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Vanittanakom","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Department of Microbiology2 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Ungpakorn","sequence":"additional","affiliation":[{"name":"<!--label omitted: 3-->Institute of Dermatology, Bangkok,3 Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E. G. V.","family":"Evans","sequence":"additional","affiliation":[{"name":"<!--label omitted: 4-->Department of Microbiology, University of Leeds, Leeds, LS2,4United Kingdom, and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Sirisanthana","sequence":"additional","affiliation":[{"name":"<!--label omitted: 5-->Department of Medicine,5 Chiang Mai University Hospital, Chiang Mai 50200, and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. J.","family":"Hay","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Dermatology Laboratory, St. John\u2019s Institute of Dermatology, Thomas Guy House, Guy\u2019s Hospital, London SE1-9RT,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"418","article-title":"P\u00e9nicillose de Rhizomys sinensis","volume":"49","author":"Capponi M.","year":"1956","unstructured":"Capponi M. Sureau P. Segretain G. P\u00e9nicillose de Rhizomys sinensis.Bull. Soc. Pathol. Exot. 49 1956 418 421","journal-title":"Bull. Soc. Pathol. Exot."},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/173.6.1490"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.9.2220-2223.1997"},{"key":"e_1_3_2_5_2","first-page":"195","article-title":"Penicilliosis due to Penicillium marneffei: a new emerging systemic mycosis in AIDS patients travelling or living in southeast Asia","volume":"4","author":"Drouhet E.","year":"1993","unstructured":"Drouhet E. Penicilliosis due to Penicillium marneffei: a new emerging systemic mycosis in AIDS patients travelling or living in southeast Asia.J. Mycol. Med. 4 1993 195 224","journal-title":"J. Mycol. Med."},{"key":"e_1_3_2_6_2","first-page":"21","article-title":"Infection a Penicillium marneffei: mycose syst\u00e9mique a manifestations cutan\u00e9es associ\u00e9e au SIDA","volume":"5","author":"Drouhet E.","year":"1995","unstructured":"Drouhet E. Dupont B. Infection a Penicillium marneffei: mycose syst\u00e9mique a manifestations cutan\u00e9es associ\u00e9e au SIDA.J. Mycol. Med. 5 1995 21 34","journal-title":"J. Mycol. Med."},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/23.1.125"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1080\/02681219080000601"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.60.1.143-149.1992"},{"key":"e_1_3_2_10_2","first-page":"466","article-title":"Disseminated Penicillium marneffei infection associated with human immunodeficiency virus: a report of two cases and a review of 35 published cases","volume":"6","author":"Hilmarsdottir I.","year":"1993","unstructured":"Hilmarsdottir I. Meynard J. L. Rogeaux O. Guermonprez G. Datry A. Katlama C. Bruker G. Coutellier A. Danis M. Gentilini M. Disseminated Penicillium marneffei infection associated with human immunodeficiency virus: a report of two cases and a review of 35 published cases.J. Acquired Immune Defic. Syndr. 6 1993 466 471","journal-title":"J. Acquired Immune Defic. Syndr."},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1080\/02681219480000071"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.8.2136-2138.1995"},{"key":"e_1_3_2_13_2","unstructured":"Latg\u00e9\nJ. P.\nBeauvais\nA.\nParis\nS.\nSarfati\nJ.\nDiaquin\nM.\nDebeaupuis\nJ. P.\nCalera\nJ. A.\nLeal\nF.\nMonod\nM. Aspergillus fumigatus pathogen or saprophyte? abstr. 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