{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T23:17:56Z","timestamp":1791328676663,"version":"4.3.1"},"reference-count":38,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[1998,9,1]],"date-time":"1998-09-01T00:00:00Z","timestamp":904608000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[1998,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The detailed analysis of 411 strains of coagulase-negative staphylococci (CoNS) obtained from 40 neutropenic hemato-oncologic patients (61 Hickman catheter episodes) on intensive chemotherapy is described. By random amplification of polymorphic DNA (RAPD) analysis, a total of 88 different genotypes were detected: 51 in air samples and 30 in skin cultures prior to insertion, 12 in blood cultures after insertion, and only 5 involved in catheter-related infections (CRI). Two RAPD genotypes of\n            <jats:italic>Staphylococcus epidermidis<\/jats:italic>\n            predominated, and their prevalence increased during patient hospitalization. At insertion, these clones constituted 11 of 86 (13%) CoNS isolated from air samples and 33 of 75 (44%) CoNS isolated from skin cultures. After insertion, their combined prevalence increased to 33 of 62 (53%) in catheters not associated with CRI and 139 of 188 (74%) in catheters associated with CRI (\n            <jats:italic>P<\/jats:italic>\n            = 0.0041). These two predominant\n            <jats:italic>S. epidermidis<\/jats:italic>\n            clones gave rise to a very high incidence of CRI (6.0 per 1,000 catheter days) and a very high catheter removal rate for CRI, 70%, despite prompt treatment with vancomycin. A likely source of\n            <jats:italic>S. epidermidis<\/jats:italic>\n            strains involved in CRI appeared to be the skin flora in 75% of cases. The validity of these observations was confirmed by pulsed-field gel electrophoresis (PFGE) of\n            <jats:italic>Sma<\/jats:italic>\n            I DNA macrorestriction fragments of blood culture CoNS isolates. Again, two predominant CoNS genotypes were found (combined prevalence, 60%). RAPD and PFGE yielded concordant results in 75% of cases. Retrospectively, the same two predominant CoNS clones were also found among blood culture CoNS isolates from the same hematology department in the period 1991 to 1993 (combined prevalence, 42%) but not in the period 1978 to 1982. These observations underscore the pathogenic potential of clonal CoNS types that have successfully and persistently colonized patients in this hemato-oncology department.\n          <\/jats:p>","DOI":"10.1128\/jcm.36.9.2696-2702.1998","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T17:36:29Z","timestamp":1577813789000},"page":"2696-2702","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":52,"title":["Clonal Expansion of\n            <i>Staphylococcus epidermidis<\/i>\n            Strains Causing Hickman Catheter-Related Infections in a Hemato-Oncologic Department"],"prefix":"10.1128","volume":"36","author":[{"given":"Jan L.","family":"Nouwen","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Department of Medical Microbiology &amp; Infectious Diseases1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alex","family":"van Belkum","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Medical Microbiology &amp; Infectious Diseases1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siem","family":"de Marie","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Medical Microbiology &amp; Infectious Diseases1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacqueline","family":"Sluijs","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Medical Microbiology &amp; Infectious Diseases1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jenne J.","family":"Wielenga","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Department of Hematology,2 Erasmus University Medical Center Rotterdam, Rotterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan A. 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W.","family":"Kluytmans","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Medical Microbiology &amp; Infectious Diseases1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henri A.","family":"Verbrugh","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Medical Microbiology &amp; Infectious Diseases1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1982.03330210050034"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002709900226"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9343(91)90349-3"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.41.5.906"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.28.3.495-503.1990"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1378\/chest.97.4.966"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1136\/adc.60.2.129"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.1996.2.423"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/10.6.1102"},{"key":"e_1_3_2_11_2","first-page":"324","article-title":"Extra hospital stay and antibiotic usage with nosocomial coagulase-negative staphylococcal bacteremia in two neonatal intensive care unit populations","volume":"144","author":"Freeman J.","year":"1990","unstructured":"Freeman J. 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