{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,13]],"date-time":"2026-06-13T03:05:40Z","timestamp":1781319940698,"version":"3.54.1"},"reference-count":24,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[1999,3,1]],"date-time":"1999-03-01T00:00:00Z","timestamp":920246400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[1999,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The 37-kDa protein (P37) of\n            <jats:italic>Borrelia burgdorferi<\/jats:italic>\n            is an antigen that elicits an early immunoglobulin M (IgM) antibody response in Lyme disease patients. The\n            <jats:italic>P37<\/jats:italic>\n            gene was cloned from a\n            <jats:italic>B. burgdorferi<\/jats:italic>\n            genomic library by screening with antibody from a Lyme disease patient who had developed a prominent humoral response to the P37 antigen. DNA sequence analysis of this clone revealed the identity of P37 to be FlaA, an outer sheath protein of the periplasmic flagella. Recombinant P37 expression was accomplished in\n            <jats:italic>Escherichia coli<\/jats:italic>\n            by using a gene construct with the leader peptide deleted and fused to a 38-kDa\n            <jats:italic>E. coli<\/jats:italic>\n            protein. The recombinant antigen was reactive in IgM immunoblots using serum samples from patients clinically diagnosed with early Lyme disease that had been scored positive for\n            <jats:italic>B. burgdorferi<\/jats:italic>\n            anti-P37 reactivity. Lyme disease patient samples serologically negative for the\n            <jats:italic>B. burgdorferi<\/jats:italic>\n            P37 protein did not react with the recombinant. Recombinant P37 may be a useful component of a set of defined antigens for the serodiagnosis of early Lyme disease. This protein can be utilized as a marker in diagnostic immunoblots, aiding in the standardization of the present generation of IgM serologic tests.\n          <\/jats:p>","DOI":"10.1128\/jcm.37.3.548-552.1999","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T17:42:55Z","timestamp":1577814175000},"page":"548-552","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":22,"title":["The\n            <i>Borrelia burgdorferi<\/i>\n            37-Kilodalton Immunoblot Band (P37) Used in Serodiagnosis of Early Lyme Disease Is the\n            <i>flaA<\/i>\n            Gene Product"],"prefix":"10.1128","volume":"37","author":[{"suffix":"Jr.","given":"Robert D.","family":"Gilmore","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U.S. Department of Health and Human Services, Fort Collins, Colorado"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rendi L.","family":"Murphree","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U.S. Department of Health and Human Services, Fort Collins, Colorado"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela M.","family":"James","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U.S. Department of Health and Human Services, Fort Collins, Colorado"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah A.","family":"Sullivan","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U.S. Department of Health and Human Services, Fort Collins, Colorado"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara J. B.","family":"Johnson","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U.S. Department of Health and Human Services, Fort Collins, Colorado"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.34.1.1-9.1996"},{"key":"e_1_3_2_3_2","unstructured":"Association of State and Territorial Public Health Laboratory Directors and the Centers for Disease Control and Prevention\nRecommendations\nProceedings of the Second National Conference on Serologic Diagnosis of Lyme disease Dearborn Mich.\n1995\n1\n7\nAssociation of State and Territorial Public Health Laboratory Directors\nWashington D.C"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.171.3.1692-1697.1989"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02098084"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/167.2.392"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.2.419-427.1995"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)80341-6"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.65.7.2992-2995.1997"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1997.tb12606.x"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.179.2.552-556.1997"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.180.9.2418-2425.1998"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.64.6.2234-2239.1996"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.58.7.2025-2034.1990"},{"key":"e_1_3_2_15_2","unstructured":"Johnson B. 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