{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T14:35:20Z","timestamp":1790519720998,"version":"4.1.0"},"reference-count":16,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[1999,3,1]],"date-time":"1999-03-01T00:00:00Z","timestamp":920246400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[1999,3]]},"abstract":"ABSTRACT<\/jats:title>\n \n A 7-year-old girl was admitted to the hospital with acute lymphoblastic leukemia and was treated with allogenic cord blood transplantation. At day 30 after graft, she developed a fever and multiple nodular lesions disseminated in the liver and lungs. All bacterial cultures attempted on liver and lung biopsy specimens and blood remained sterile on standard axenic media. However, inoculation of liver and lung biopsy specimens on eukaryotic cell monolayers by the centrifugation-shell vial technique (M. Marrero and D. Raoult, Am. J. Trop. Med. Hyg. 40:197\u2013199, 1989) led to the recovery of a strain of\n Legionella pneumophila<\/jats:italic>\n serogroup 1, identified by 16S rRNA gene amplification and sequencing and serotyping. Our findings demonstrate that the centrifugation-cell culture method, which has previously been useful for the isolation of other strictly or facultatively intracellular bacteria, can also serve as a method for the recovery of\n L. pneumophila<\/jats:italic>\n from clinical material.\n <\/jats:p>","DOI":"10.1128\/jcm.37.3.785-787.1999","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T17:43:24Z","timestamp":1577814204000},"page":"785-787","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":31,"title":["Isolation of\n Legionella pneumophila<\/i>\n by Centrifugation of Shell Vial Cell Cultures from Multiple Liver and Lung Abscesses"],"prefix":"10.1128","volume":"37","author":[{"given":"Bernard","family":"La Scola","sequence":"first","affiliation":[{"name":"Unite\u0301 des Rickettsies, CNRS UPRESA 6020, Faculte\u0301 de Me\u0301decine, 13385 Marseille Cedex 05,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ge\u0301rard","family":"Michel","sequence":"additional","affiliation":[{"name":"Service d\u2019Onco-He\u0301matologie Pe\u0301diatrique, Ho\u0302pital de la Timone, 13005 Marseille,2 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Didier","family":"Raoult","sequence":"additional","affiliation":[{"name":"Unite\u0301 des Rickettsies, CNRS UPRESA 6020, Faculte\u0301 de Me\u0301decine, 13385 Marseille Cedex 05,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/clind\/16.6.741"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.9.2782-2783.1998"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199212033272303"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.34.11.2722-2727.1996"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1086\/513631"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1989.40.197"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197712012972202"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-90-4-659"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.12.3129-3132.1995"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.63.4.1219-1224.1997"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-125-8-199610150-00004"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.22.3.422-424.1985"},{"key":"e_1_3_1_14_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.36.9.978"},{"key":"e_1_3_1_15_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199709043371006"},{"key":"e_1_3_1_16_2","unstructured":"Winn\nW. C.\nJr. Legionella Manual of clinical microbiology\n6th ed.\nMurray\nP. R.\nBaron\nE. J.\nPfaller\nM. A.\nTenover\nF. C.\nYolken\nR. H.\n1995\n533\n544\nASM Press\nWashington D.C"},{"key":"e_1_3_1_17_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1983.03340150023020"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.37.3.785-787.1999","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.37.3.785-787.1999","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T00:00:20Z","timestamp":1646438420000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.37.3.785-787.1999"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,3]]},"references-count":16,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1999,3]]}},"alternative-id":["10.1128\/JCM.37.3.785-787.1999"],"URL":"https:\/\/doi.org\/10.1128\/jcm.37.3.785-787.1999","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1999,3]]},"assertion":[{"value":"1998-10-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1998-12-09","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"1999-03-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}