{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T09:23:11Z","timestamp":1792660991215,"version":"4.5.0"},"reference-count":24,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2000,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Species-specific identification of human tapeworm infections is important for public health purposes, because prompt identification of\n            <jats:italic>Taenia solium<\/jats:italic>\n            carriers may prevent further human cysticercosis infections (a major cause of acquired epilepsy). Two practical methods for the differentiation of cestode proglottids, (i) routine embedding, sectioning, and hematoxylin-eosin (HE) staining and (ii) PCR with restriction enzyme analysis (PCR-REA), were tested on samples from 40 individuals infected with\n            <jats:italic>T. solium<\/jats:italic>\n            (\n            <jats:italic>n<\/jats:italic>\n            = 34) or\n            <jats:italic>Taenia saginata<\/jats:italic>\n            (\n            <jats:italic>n<\/jats:italic>\n            = 6). Microscopic examination of HE staining of sections from 24 cases, in which conserved proglottids were recovered, clearly revealed differences in the number of uterine branches. Distinct restriction patterns for\n            <jats:italic>T. solium<\/jats:italic>\n            and\n            <jats:italic>T. saginata<\/jats:italic>\n            were observed when the PCR products containing the ribosomal 5.8S gene plus internal transcribed spacer regions were digested with either\n            <jats:italic>Alu<\/jats:italic>\n            I,\n            <jats:italic>Dde<\/jats:italic>\n            I, or\n            <jats:italic>Mbo<\/jats:italic>\n            I. Both HE histology and PCR-REA are useful techniques for differentiating\n            <jats:italic>T. solium<\/jats:italic>\n            from\n            <jats:italic>T. saginata<\/jats:italic>\n            . Importantly, both techniques can be used in zones of endemicity. HE histology is inexpensive and is currently available in most regions of endemicity, and PCR-REA can be performed in most hospital centers already performing PCR without additional equipment or the use of radioactive material.\n          <\/jats:p>","DOI":"10.1128\/jcm.38.1.133-137.2000","type":"journal-article","created":{"date-parts":[[2021,5,29]],"date-time":"2021-05-29T21:44:29Z","timestamp":1622324669000},"page":"133-137","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":86,"title":["Differentiating\n            <i>Taenia solium<\/i>\n            and\n            <i>Taenia saginata<\/i>\n            Infections by Simple Hematoxylin-Eosin Staining and PCR-Restriction Enzyme Analysis"],"prefix":"10.1128","volume":"38","author":[{"given":"H.","family":"Mayta","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Infectious Diseases Laboratory, Department of Pathology,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Talley","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Mount Sinai School of Medicine, New York, New York2; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. H.","family":"Gilman","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Infectious Diseases Laboratory, Department of Pathology,1 and"},{"name":"<!--label omitted: 3-->Department of International Health, Johns Hopkins University School of Hygiene and Public Health, Baltimore, Maryland3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Jimenez","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Infectious Diseases Laboratory, Department of Pathology,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Verastegui","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Infectious Diseases Laboratory, Department of Pathology,1 and"},{"name":"<!--label omitted: 4-->Department of Microbiology,4 Universidad Peruana Cayetano Heredia,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Ruiz","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Infectious Diseases Laboratory, Department of Pathology,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. H.","family":"Garcia","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Infectious Diseases Laboratory, Department of Pathology,1 and"},{"name":"<!--label omitted: 4-->Department of Microbiology,4 Universidad Peruana Cayetano Heredia,"},{"name":"<!--label omitted: 5-->Department of Transmissible Diseases, Instituto de Ciencias Neurolo\u0301gicas,5 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. E.","family":"Gonzalez","sequence":"additional","affiliation":[{"name":"<!--label omitted: 6-->Department of Public Health, School of Veterinary Medicine, Universidad Nacional Mayor de San Marcos,6 Lima, Peru;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000061801"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(93)90199-8"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000076332"},{"key":"e_1_3_2_5_2","unstructured":"Brunton\nL. L.\nAgents affecting gastrointestinal water flux and motility digestants and bile acids\nThe pharmacological basis of therapeutics\n8th ed.\nGoodman\nA.\nRall\nT. W.\nNeis\nA. S.\nTaylor\nP.\n1990\n922\nPergamon Press\nNew York N.Y"},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","unstructured":"Bryan\nR. T.\nCurrent issues in cysticercosis: proteins proglottids pigs and prives\nGlobal infectious diseases: prevention control and eradication.\nWalker\nD. H.\n1992\n181\n203\nSpringer-Verlag\nVienna Austria","DOI":"10.1007\/978-3-7091-3449-8_11"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0020-7519(80)90044-2"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.5.1283-1288.1995"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/10.6.1075"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(88)90626-5"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0169-4758(88)90187-1"},{"key":"e_1_3_2_12_2","first-page":"77","article-title":"Taeniasis and cysticercosis due to Taenia solium","volume":"4","author":"Flisser A.","year":"1994","unstructured":"Flisser A. Taeniasis and cysticercosis due to Taenia solium.Prog. Clin. Parasitol.4199477116","journal-title":"Prog. Clin. 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Hyg."},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0020-7519(78)90068-1"},{"key":"e_1_3_2_18_2","first-page":"32","article-title":"The hamster (Mesocricetus auratus) as an experimental definitive host of Taenia solium","volume":"30","author":"Letonja T.","year":"1975","unstructured":"Letonja T. The hamster (Mesocricetus auratus) as an experimental definitive host of Taenia solium.Bol. Chil. Parasitol.3019753233","journal-title":"Bol. Chil. Parasitol."},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.2307\/3280839"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.2307\/3276706"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000054020"},{"key":"e_1_3_2_22_2","first-page":"1275","article-title":"DNA probes which unambiguously distinguish Taenia solium from T. saginata","author":"Rishi A. K.","year":"1988","unstructured":"Rishi A. K. McManus D. P. 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