{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T20:52:46Z","timestamp":1789937566942,"version":"4.0.1"},"reference-count":24,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2000,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Neurocysticercosis (NC), i.e., the presence of the larval form of\n            <jats:italic>Taenia solium<\/jats:italic>\n            in tissues, is the most frequent and severe infection involving the central nervous system. Paired serum and cerebrospinal fluid (CSF) samples from patients with NC, CSF and serum samples from a control group, and serum samples from patients with other parasitoses were studied by enzyme-linked immunosorbent assay (ELISA) and by immunoblotting with\n            <jats:italic>Taenia crassiceps<\/jats:italic>\n            vesicular fluid antigen (Tcra) and\n            <jats:italic>Taenia solium<\/jats:italic>\n            total saline antigen (Tso) for the detection of immunoglobulin G antibodies. ELISAs carried out with the Tso and Tcra antigens showed 94.1 and 95.6% sensitivities, respectively, for the detection of antibodies in CSF and 70.6% and 91.2% sensitivities, respectively, for the detection of antibodies in serum, with 100% specificity for the detection of antibodies in CSF and 80% specificity for the detection of antibodies in serum for both antigens. On the basis of the reactivities of the peptides in the samples analyzed, the peptides of \u226423, 39, 85 to 77, and 97 kDa were found to be Tso specific by immunoblotting and the peptides of \u226462, 74, 109, 121, and 131 kDa were found to be Tcra specific. Tests with Tcra extract had higher sensitivities and more homogeneous results and permitted us to obtain the parasites easily. We suggest the use of Tcra ELISA for the study of serum and confirmation of the results for sera positive by an immunoblotting analysis in which specific peptides (e.g., peptides of 19 to 13 kDa) are detected.\n          <\/jats:p>","DOI":"10.1128\/jcm.38.1.146-151.2000","type":"journal-article","created":{"date-parts":[[2021,5,29]],"date-time":"2021-05-29T21:44:29Z","timestamp":1622324669000},"page":"146-151","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":50,"title":["Specific\n            <i>Taenia crassiceps<\/i>\n            and\n            <i>Taenia solium<\/i>\n            Antigenic Peptides for Neurocysticercosis Immunodiagnosis Using Serum Samples"],"prefix":"10.1128","volume":"38","author":[{"given":"Edne\u0301ia Casagranda","family":"Bueno","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Laboratory of Clinical Immunology, Faculty of Pharmaceutical Sciences,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adelaide Jose\u0301","family":"Vaz","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Laboratory of Clinical Immunology, Faculty of Pharmaceutical Sciences,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lui\u0301s Dos Ramos","family":"Machado","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Center of Neurological Investigation, Faculty of Medicine,2 University of Sa\u0303o Paulo, Sa\u0303o Paulo, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose\u0301Anto\u0302nio","family":"Livramento","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Center of Neurological Investigation, Faculty of Medicine,2 University of Sa\u0303o Paulo, Sa\u0303o Paulo, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Si\u0301lvia Regina","family":"Mielle","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Center of Neurological Investigation, Faculty of Medicine,2 University of Sa\u0303o Paulo, Sa\u0303o Paulo, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0036-46651996000300008"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0036-46651996000400004"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.24.4.536-541.1986"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1136\/jnnp.52.2.254"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0036-46651997000100006"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.12.3324-3325.1995"},{"key":"e_1_3_2_8_2","first-page":"299","article-title":"Species-specific immunodiagnosis of Taenia solium cystecercosis by ELISA and immunoblotting","volume":"38","author":"Gottstein B.","year":"1987","unstructured":"Gottstein B. Zini D. Schantz P. M. Species-specific immunodiagnosis of Taenia solium cystecercosis by ELISA and immunoblotting.Trop. Med. Parasitol.381987299303","journal-title":"Trop. Med. Parasitol."},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.2307\/3281534"},{"key":"e_1_3_2_10_2","first-page":"510","article-title":"Taenia crassiceps metacestode vesicular fluid antigens shared with the Taenia solium larval stage and reactive with serum antibodies from patients with neurocysticercosis","volume":"271","author":"Kunz J.","year":"1989","unstructured":"Kunz J. Kalinna B. Watschke V. Geyer E. Taenia crassiceps metacestode vesicular fluid antigens shared with the Taenia solium larval stage and reactive with serum antibodies from patients with neurocysticercosis.Zentbl. Bakteriol. Parasitenkd. Infektkrankh. Hyg. Abt. 1 Orig.2711989510520","journal-title":"Zentbl. Bakteriol. Parasitenkd. Infektkrankh. Hyg. Abt. 1 Orig."},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"e_1_3_2_12_2","first-page":"926","article-title":"Immunodiagnosis of human cysticercosis in cerebrospinal fluid. Antigens from murine Taenia crassiceps cysticerci effectively substitute those from porcine Taenia solium","volume":"114","author":"Larralde C.","year":"1990","unstructured":"Larralde C. Sotelo J. Montoya R. M. Palencia G. Padilla A. Govezensky T. Diaz M. L. Sciutto E. Immunodiagnosis of human cysticercosis in cerebrospinal fluid. Antigens from murine Taenia crassiceps cysticerci effectively substitute those from porcine Taenia solium.Arch. Pathol. Lab. Med.1141990926928","journal-title":"Arch. Pathol. Lab. Med."},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0004-282X1990000400003"},{"key":"e_1_3_2_14_2","first-page":"119","article-title":"Apport de l'enzyme-linked immunoelectrotransfer blot assay au diagnostic de la neurocysticercose humaine","volume":"38","author":"Michault A.","year":"1990","unstructured":"Michault A. Rivi\u00e8re B. Fressy P. Laporte J. P. Bertil G. Mignard C. Apport de l'enzyme-linked immunoelectrotransfer blot assay au diagnostic de la neurocysticercose humaine.Pathol. Biol.381990119125","journal-title":"Pathol. Biol."},{"key":"e_1_3_2_15_2","first-page":"115","article-title":"Le serodiagnostic de la cysticercose par ELISA et Western blot. Son int\u00e9r\u00eat et ses limites \u00e0 Madagascar","volume":"57","author":"Michel P.","year":"1990","unstructured":"Michel P. Michault A. Gruel J. C. Coulanges P. Le serodiagnostic de la cysticercose par ELISA et Western blot. Son int\u00e9r\u00eat et ses limites \u00e0 Madagascar.Arch. Inst. Pasteur Madagascar571990115142","journal-title":"Arch. Inst. Pasteur Madagascar"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1997.56.127"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199209033271004"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1080\/00034983.1991.11812573"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0004-282X1997000300001"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.9.4350"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/159.1.50"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1997.57.354"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0888-0786(96)01071-2"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/164.5.1007"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(1950)3:1<32::AID-CNCR2820030106>3.0.CO;2-3"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.38.1.146-151.2000","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.38.1.146-151.2000","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T00:30:08Z","timestamp":1646440208000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.38.1.146-151.2000"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,1]]},"references-count":24,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2000,1]]}},"alternative-id":["10.1128\/JCM.38.1.146-151.2000"],"URL":"https:\/\/doi.org\/10.1128\/jcm.38.1.146-151.2000","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,1]]},"assertion":[{"value":"1999-07-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1999-10-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2000-01-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}