{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T03:49:48Z","timestamp":1780631388282,"version":"3.54.1"},"reference-count":17,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2000,10,1]],"date-time":"2000-10-01T00:00:00Z","timestamp":970358400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2000,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Previously,\n            <jats:italic>Rhodotorula glutinis<\/jats:italic>\n            was reported to produce a large amount of exocellular mannan, having a repeating unit of \u21923)-\n            <jats:sc>d<\/jats:sc>\n            -Man\n            <jats:italic>p<\/jats:italic>\n            -(1\u21924)-\n            <jats:sc>d<\/jats:sc>\n            -Man\n            <jats:italic>p<\/jats:italic>\n            -(1\u2192. Recently, we found that antigenic polysaccharides of\n            <jats:italic>Leptospira biflexa<\/jats:italic>\n            serovar patoc strain Patoc I have the same repeating unit and cross-react with antisera raised against extended strains of other leptospires (K. Matsuo, E. Isogai, and Y. Araki, Carbohydr. Res., in press). This structural identity and the difficulty of producing and isolating antigens led us to confirm the usefulness of\n            <jats:italic>Rhodotorula<\/jats:italic>\n            mannan as an immunoreactive antigen in a serological diagnosis of leptospirosis. In the present investigation, we confirmed the structural identity of an exocellular mannan isolated from\n            <jats:italic>R. glutinis<\/jats:italic>\n            AHU 3479 and tried to use it as an immunoreactive antigen in a serological diagnosis of leptospirosis. From its chemical analysis and\n            <jats:sup>1<\/jats:sup>\n            H- and\n            <jats:sup>13<\/jats:sup>\n            C-labeled nuclear magnetic resonance spectrometry, the\n            <jats:italic>Rhodotorula<\/jats:italic>\n            mannan was confirmed to consist of the same disaccharide units. Furthermore, such a preparation was shown to immunoreact to various sera from patients suffering with leptospirosis as well as to most rabbit antiserum preparations obtained from immunization with various strains of pathogenic leptospires. Therefore, the\n            <jats:italic>Rhodotorula<\/jats:italic>\n            mannan preparation is useful as an immunoreactive antigen in the serological diagnosis for leptospirosis.\n          <\/jats:p>","DOI":"10.1128\/jcm.38.10.3750-3754.2000","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T17:49:57Z","timestamp":1577814597000},"page":"3750-3754","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":16,"title":["Utilization of Exocellular Mannan from\n            <i>Rhodotorula glutinis<\/i>\n            as an Immunoreactive Antigen in Diagnosis of Leptospirosis"],"prefix":"10.1128","volume":"38","author":[{"given":"Kouki","family":"Matsuo","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Laboratory of Environmental Molecular Biology, Graduate School of Environmental Earth Science, Hokkaido University, Sapporo,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emiko","family":"Isogai","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Department of Preventive Dentistry, Health Sciences University of Hokkaido, Ishikari-Tobetsu,2 Hokkaido, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshio","family":"Araki","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Laboratory of Environmental Molecular Biology, Graduate School of Environmental Earth Science, Hokkaido University, Sapporo,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.11.5.452-457.1980"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac60111a017"},{"key":"e_1_3_1_4_2","unstructured":"Edwards\nT. E.\nYeast polysaccharide: isolation of glycogen glucan and mannan polysaccharides from Baker's yeast\nMethods in carbohydrate chemistry vol. V: general polysaccharides.\nWhistler\nR. L.\n1965\n176\n179\nAcademic Press Inc.\nNew York N.Y"},{"key":"e_1_3_1_5_2","unstructured":"Faine\nS.\nAdler\nB.\nBolin\nC.\nPerolat\nP.\nLeptospira and leptospirosis\n2nd ed.\n1999\nMediSci\nMelbourne Australia"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1139\/v65-122"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.1.92-97.1997"},{"key":"e_1_3_1_8_2","first-page":"53","article-title":"Biological activities of leptospiral lipopolysaccharide","volume":"261","author":"Isogai E.","year":"1986","unstructured":"Isogai E. Isogai H. Kurebayashi Y. Ito N. Biological activities of leptospiral lipopolysaccharide.Zentbl. Bakteriol. Mikrobiol. Hyg. Ser. A.26119865364","journal-title":"Zentbl. Bakteriol. Mikrobiol. Hyg. Ser. A."},{"key":"e_1_3_1_9_2","doi-asserted-by":"crossref","unstructured":"Matsuo\nK.\nIsogai\nE.\nAraki\nY.\nOccurrence of [\u21923)-\u03b2- d -Manp-(1\u21924)-\u03b2- d -Manp-(1\u2192] n units in the antigenic polysaccharides from Leptospira biflexa serovar patoc strain Patoc I.\n2000Carbohydr. Res. in press.","DOI":"10.1016\/S0008-6215(00)00143-9"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.26.1.7"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-82-2-223"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(75)41003-5"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.9.2904-2909.1999"},{"key":"e_1_3_1_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.3.1272-1275.2000"},{"key":"e_1_3_1_15_2","first-page":"377","article-title":"ELISA for the detection of specific IgM and IgG in human leptospirosis","volume":"131","author":"Terpstra W. 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