{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T04:01:01Z","timestamp":1780632061380,"version":"3.54.1"},"reference-count":26,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2000,2,1]],"date-time":"2000-02-01T00:00:00Z","timestamp":949363200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2000,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The\n            <jats:italic>Helicobacter pylori iceA<\/jats:italic>\n            gene was recently identified as a genetic marker for the development of peptic ulcer in a Western population. To assess the significance of\n            <jats:italic>iceA<\/jats:italic>\n            subtypes of\n            <jats:italic>H. pylori<\/jats:italic>\n            in relation to peptic ulcer, 140 Japanese clinical isolates (88 from Fukui and 52 from Okinawa) were characterized. Sequence analysis of the\n            <jats:italic>iceA1<\/jats:italic>\n            gene from 25 representative Japanese strains was also carried out to identify the differences in\n            <jats:italic>iceA<\/jats:italic>\n            between the ulcer group and the gastritis group. The\n            <jats:italic>iceA1<\/jats:italic>\n            genotype was not correlated with the presence of peptic ulceration in either area. In addition, sequence analysis led to identification of five deletions and five point mutations (a nonsense mutation or a 1-bp insertion) within the\n            <jats:italic>iceA1<\/jats:italic>\n            open reading frame corresponding to previously published sequences. These mutations were identified in both clinical groups (ulcer and gastritis groups) in each area. Local DNA sequence analysis revealed that the endpoints of all five deletions coincided with direct repeats. We also found four strains that carried longer\n            <jats:italic>iceA1<\/jats:italic>\n            open reading frames compared with that for strain 60190. In conclusion, carriage of an\n            <jats:italic>iceA1<\/jats:italic>\n            strain does not seem to be a risk factor for peptic ulcer in Japanese subjects. The critical mutations in the\n            <jats:italic>iceA1<\/jats:italic>\n            gene in some isolates from patients with peptic ulcers suggested that IceA does not participate in the pathogenesis of peptic ulcer in Japan. We also found deletion hot spots that were associated with direct repeats in\n            <jats:italic>iceA1<\/jats:italic>\n            and that favored a small-deletion model of slipped mispairing events during replication. We showed that\n            <jats:italic>iceA1<\/jats:italic>\n            sequence variations may be useful tools for analysis of the population genetics of\n            <jats:italic>H. pylori<\/jats:italic>\n            .\n          <\/jats:p>","DOI":"10.1128\/jcm.38.2.483-488.2000","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T17:52:13Z","timestamp":1577814733000},"page":"483-488","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":35,"title":["Sequence Analysis and Clinical Significance of the\n            <i>iceA<\/i>\n            Gene from\n            <i>Helicobacter pylori<\/i>\n            Strains in Japan"],"prefix":"10.1128","volume":"38","author":[{"given":"Yoshiyuki","family":"Ito","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Second Department of Internal Medicine, Fukui Medical University,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeshi","family":"Azuma","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Second Department of Internal Medicine, Fukui Medical University,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigeji","family":"Ito","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Second Department of Internal Medicine, Fukui Medical University,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroyuki","family":"Suto","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Second Department of Internal Medicine, Fukui Medical University,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideki","family":"Miyaji","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Second Department of Internal Medicine, Fukui Medical University,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukinao","family":"Yamazaki","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Second Department of Internal Medicine, Fukui Medical University,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takuji","family":"Kato","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Fukui Prefectural University, College of Nursing,2Fukui,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshihiro","family":"Kohli","sequence":"additional","affiliation":[{"name":"<!--label omitted: 3-->Division of Internal Medicine, Aiseikai Yamashina Hospital, Kyoto,3 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshihide","family":"Keida","sequence":"additional","affiliation":[{"name":"<!--label omitted: 4-->Division of Internal Medicine, Okinawa Chubu Hospital, Okinawa,4 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masaru","family":"Kuriyama","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Second Department of Internal Medicine, Fukui Medical University,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01382.x"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1998.00770.x"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.30.17771"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.25.14648"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(99)70553-6"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.58.3.603-610.1990"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(91)90477-7"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(78)90023-2"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.27.1.225-226.1989"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1998.00806.x"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199302043280503"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.7.1710-1714.1997"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1086\/314435"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(96)00561-6"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1086\/515601"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199110173251603"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199405053301803"},{"key":"e_1_3_2_19_2","first-page":"531","article-title":"Adherence to gastric epithelial cells induces expression of a Helicobacter pylori gene, iceA, that is associated with clinical outcome","volume":"110","author":"Peek R. 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